FastQCFastQC Report
Thu 14 Sept 2023
SRR14714420_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR14714420_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45917201
Total Bases3.9 Gbp
Sequences flagged as poor quality0
Sequence length85
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCCTTTAACGAGGATCCATTGGAGGGCAAGTCTGGTGCCAGCAGCCGCG3870180.8428606090340742No Hit
AGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGCAAAAAAAAAAAAA2675720.5827271570843353No Hit
ATCTGTGGGATTATGACTGAACGCCTCTAAGTCAGAATCCCGCCCAGGCG2577320.561297279422585No Hit
GTGGGATTATGACTGAACGCCTCTAAGTCAGAATCCCGCCCAGGCGGAAC2367990.5157086992301643No Hit
GAAAGGGAGTCGGGTTCAGATCCCCGAATCCGGAGTGAAAAAAAAAAAAA1833110.39922076260702394No Hit
GGGGCCCGAGGTGGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACC1662840.36213879848643216No Hit
ATTCCCCATGAACGAGGAATTCCCAGTAAGTGCGGGTCATAAGCTTGCGT1543330.3361115151596457No Hit
AAAGGAATTGACGGAAGGGCACCACCAGGAGTGGAGCCTGCGGCTTAAAA1492310.325000210705352No Hit
GATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAAAAAAAAAA1393880.30356379954431456No Hit
CGTCTTGGGAAACGGGGTGCGGCCGGAAAGGGGGCCGCCCTCTCGCCCGT1386470.30195002522039616No Hit
CTGTGGGATTATGACTGAACGCCTCTAAGTCAGAATCCCGCCCAGGCGGA1317880.2870122680169464No Hit
GAAGGGCAGGGCGCCCTGGAATGGGTTCGCCCCGAGAGAGGGGCCCGTGC1199620.2612572138271233No Hit
GTCTTGGGAAACGGGGTGCGGCCGGAAAGGGGGCCGCCCTCTCGCCCGTC1139990.24827079507742641No Hit
ACGGGAAACCTCACCCGGCCCGGACACGGACAGGATTGACAAAAAAAAAA1128500.24576846485045983No Hit
GAAAGATGGTGAACTATGCCTGGGCAGGGCGAAGCCAGAGGAAAAAAAAA1099900.23953986219673973No Hit
AATTATTCCCCATGAACGAGGAATTCCCAGTAAGTGCGGGTCAAAAAAAA1061390.23115302694517464No Hit
GGCCCGAGGTGGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCAC1054980.2297570359308269No Hit
TGTGGGATTATGACTGAACGCCTCTAAGTCAGAATCCCGCCCAGGCGGAA1049900.22865069671820806No Hit
AAGAAGCCCGGCTCGCTGGCGTGGAGCCGGGCGTGGAAAAAAAAAAAAAA1005130.2189005379487308No Hit
CATCTGTGGGATTATGACTGAACGCCTCTAAGTCAGAATCCCGCCCAGGC989890.21558152031087435No Hit
AGCGGGGAAAGAAGACCCTGTTGAGCTTGACTCTAGTCTGGCACGGTGAA946820.20620159316766715No Hit
AGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAATCAAGAACGAAAGTC936870.20403464923743936No Hit
CGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTACCA918850.20011019399897656No Hit
TCGTCTTGGGAAACGGGGTGCGGCCGGAAAGGGGGCCGCCCTCTCGCCCG885550.19285800979027445No Hit
GTGAAGGGCAGGGCGCCCTGGAATGGGTTCGCCCCGAGAGAGGGGCCCGT847170.18449948636895355No Hit
AATTATTCCCCATGAACGAGGAATTCCCAGTAAGTGCGGGTCATAAGCTT835180.18188826448720163No Hit
GAAAGGGAGTCGGGTTCAGATCCCCGAATCCGGAGTAAAAAAAAAAAAAA825400.17975834371960084No Hit
TGGGATTATGACTGAACGCCTCTAAGTCAGAATCCCGCCCAGGCGGAACG764770.16655414166033333No Hit
AAGGCGAGCTCAGGGAGGACAGAAACCTCCCGTGGAGCAGAAGGGCAAAA739220.16098977810080364No Hit
GGGATTATGACTGAACGCCTCTAAGTCAGAATCCCGCCCAGGCGGAACGA719320.15665589024034804No Hit
TCTGTGGGATTATGACTGAACGCCTCTAAGTCAGAATCCCGCCCAGGCGG671040.14614131205427788No Hit
GAAAGGGAGTCGGGTTCAGATCCCCGAATCCGGAGTGGCGGAGATGGGCG655180.14268726876448762No Hit
AGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAACAAAAAAAAAAAAA648980.1413370122451497No Hit
CAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAG558010.12152526457350916No Hit
ATCCTTTAACGAGGATCCATTGGAGGGCAAGTCTGGTGCCAGCAAAAAAA533970.11628975381143115No Hit
AGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAAAAAAAAAAAAAAAT531000.11564293738200637No Hit
AATTAAAACAAAGCATCGCGAAGGCCCGCGGCGGGTGTTGACGCGATGTG523300.11396600589831249No Hit
CCATGACCCGCCGGGCAGCTTCCGGGAAACCAAAGTCTTTGGGTTCCGGG509700.11100415288815187No Hit
GCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCCATTCGTGA507160.11045098328184247No Hit
AGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGCAATAAAAAAAAAA502280.10938820073113777No Hit
AAAGGAATTGACGGAAGGGCACCACCAGGAGTGGAGCCTGCGGCTTAATT498210.10850182266118528No Hit
GGGGAGGTGGAGCACGAGCGTACGCGTTAGGACCCGAAAGATGGTGAACT495310.10787025106343046No Hit
ACGGGAAACCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAG490650.10685538084083131No Hit
ACGGGAAACCTCACCCGGCCCGGACACGGACAAAAAAAAAAAAAAATACA463460.10093385265360579No Hit
CTTGGGAAACGGGGTGCGGCCGGAAAGGGGGCCGCCCTCTCGCCCGTCAC460050.10019121156796994No Hit
ATCCTTTAACGAGGATCCATTGGAGGGCAAGTCTGGTGCCAAAAAAAAAA459260.10001916275340912No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)