FastQCFastQC Report
Thu 14 Sept 2023
SRR14714422_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR14714422_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58748800
Total Bases4.9 Gbp
Sequences flagged as poor quality0
Sequence length84
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAAAAAAAATTG11974372.038232270276159No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAAAAAAAATT10879441.8518573996405032No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAAAAAAATTG10554321.7965166948090854No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAATTG8345121.4204749714036713No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAATTGG7236981.231851544201754No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAAAAAAATTGG7185921.223160302848739No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAAAAAAAAAAAAAAA6696861.139914347186666No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAAAAAAAAAAAAAAAATTGG6563631.1172364371697805No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTAAAAAAAAAAAAAAAATT6192871.0541270630208617No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACAAAAAAAAAAAAAAAA5565510.9473402009913393No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAAAAAATTGG5055520.8605316193692467No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAAAAAAAAAAAAAA4962420.844684487172504No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTAAAAAAAAAAAAAAATTG4343470.7393291437442127No Hit
TCGTTTTTTCACTGACCCGGTGAGGCGGGGAAAAAAAAAAAAAAAATTGG4071630.6930575603246364No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAAAAAAAAATT3824680.6510226591862303No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAATTGGC3046730.5186029331662945No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCACAAAAAAAAAAAAAAAATTG3027150.5152700991339397No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAAATT2975550.5064869410098589No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAAAAAAAAAT2923220.49757952502859637No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCAAAAAAAAAAAAAAAATTGGC2784170.4739109564791111No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAAAAAAAAAAAAAAATTGGC2740240.46643335693665233No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAAAAAATTGGC2547510.43362758047823957No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTAAAAAAAAAAAAAAAAAT2324380.3956472302412985No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAAAAAAAAAAAAAAAAATTG2311430.3934429298981426No Hit
CCCGCCACGCAGTTTTATCCGGTAAAGCGAAAAAAAAAAAAAAAATTGGC2134860.36338784792200013No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAATT1887920.321354648946021No Hit
TCGTTTTTTCACTGACCCGGTGAGGCGGGGAAAAAAAAAAAAAAATTGGC1877050.3195043983877118No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATAAAAAAAAAAAAAAAA1780260.3030291682553516No Hit
TCGTTTTTTCACTGACCCGGTGAGGCGGGGAAAAAAAAAAAAAAAAATTG1734200.2951890081159105No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCAAAAAAAAAAAAAAATTGGCA1725400.2936911051800207No Hit
CCCCGCCACGCAGTTTTATCCGGTAAAGCGAAAAAAAAAAAAAAAATTGG1716360.2921523503458794No Hit
CCCCCCGAGTGTTACAGCCCCCAAAAAAAAAAAAAAAATTGGCAGATCGG1649200.28072062748515714No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAATTG1635350.27836313252355793No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTAAAAAAAAAAAAAAAAT1629450.2773588566915409No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACAAAAAAAAAAAAAAAT1597600.2719374693610763No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAAAAATTGGC1578440.26867612615066183No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCACAAAAAAAAAAAAAAATTGG1525220.2596172177133831No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTAAAAAAAAAAAAAATTGG1507270.2565618361566534No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGTAAAAAAAAAAAAAAAATTG1493460.2542111498447628No Hit
CCCGCCACGCAGTTTTATCCGGTAAAGCGAAAAAAAAAAAAAAATTGGCA1190740.20268328885015524No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCTCAAAAAAAAAAAAAAAATTG1187330.20210285146249796No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCACAAAAAAAAAAAAAAAAATT1150110.19576740290865516No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTAAAAAAAAAAAAAAATT1134130.19304734735007353No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAAAAAAAAAAT1103690.1878659649218367No Hit
CGGCAGCAGCACTCGCCGAATCCCGGGGCCGAGGGAGCGAAAAAAAAAAA1059160.1802862356337491No Hit
CCCCCCGAGTGTTACAGCCCCCAAAAAAAAAAAAAAATTGGCAGATCGGA1042610.1774691568168201No Hit
CCCCGCCACGCAGTTTTATCCGGTAAAGCGAAAAAAAAAAAAAAATTGGC960610.16351142491421103No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAAAAAAAATTG946220.16106201318154584No Hit
TTGGATAACTGTGGTAATTCTAGAGCTAATACATGCAAAAAAAAAAAAAA937320.15954708862138461No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTGAAAAAAAAAAAAAAAATT929220.15816833705539515No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAAAAAAATTGG923560.1572049131216297No Hit
AGAGGTCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAAAA906750.15434357808159485No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGAAAAAAAAAAAAAA896480.15259545726891444No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAATTGGCA890190.15152479710223868No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCAAAAAAAAAAAAAAAATT881120.14998093578081595No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAAAAT868640.14785663707173594No Hit
CGGCAGCAGCACTCGCCGAATCCCGGGGCCGAGGGAGCAAAAAAAAAAAA862910.14688129800098046No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCAAAAAAAAAAAAAAAAATTGG861860.14670257094612996No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTAAAAAAAAAAAAAAAAAA834170.14198928318535867No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAAAAAAAAAA828910.14109394574867912No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTAAAAAAAAAAAAAAAAA814280.13860368211776242No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCAAAAAAAAAAAAAAAA814090.13857134103164662No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAAAAAAAAATTG787330.13401635437660003No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGTAAAAAAAAAAAAAAATTGG781860.1330852715289504No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCTCAAAAAAAAAAAAAAATTGG780330.1328248406775968No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCTAAAAAAAAAAAAAAAATT729660.1241999836592407No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAAAAAAAAAAAAAAAATTG726300.12362805708371916No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAAAT711340.12108162209270656No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAAAAAAAAAAAAAATTGGCA690850.11759389127948146No Hit
CCCCCCGAGTGTTACAAAAAAAAAAAAAAAATTGGCAGATCGGAAGAGCA690540.11754112424423988No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAATTGG688760.11723813933220764No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAAAAATTGGCA687800.11707473173920148No Hit
CCCCCCGAGTGTTAAAAAAAAAAAAAAAATTGGCAGATCGGAAGAGCACA643970.10961415382101422No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGAAAAAAAAAAAAAAAATTGG642290.10932819053325343No Hit
GGATGGTTTAGTGAGGCCCTCGGATCGGCAAAAAAAAAAAAAAAATTGGC637950.10858945203987146No Hit
CCCGCCACGCAGTTTTATCCGGTAAAGCGAAAAAAAAAAAAAAAAATTGG633440.10782177678522796No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAAAAAAAAAAAAAAAAAATT593790.10107270276158832No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAATAGAAAAAAAAAAAAAAA590190.10045992428781524No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)