FastQCFastQC Report
Thu 14 Sept 2023
SRR14714423_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR14714423_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50815273
Total Bases4.2 Gbp
Sequences flagged as poor quality0
Sequence length84
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAATTCT2909330.5725306247985719No Hit
CGGCGGCGTCCGGTGAGCTCTCGCTGGCCCTTGAAAATCCGGGGGAGAGG2255250.4438134180642895No Hit
AAGGCGAGCTCAGGGAGGACAAAAAAAAAAAAAAAGCCTAAAGATCGGAA2222610.437390152366199No Hit
GAAAGGGAGTCGGGTTCAAAAAAAAAAAAAAAGCCTAAAGATCGGAAGAG1964160.38652945936155847No Hit
AGAGGTCTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1436210.282633530277403No Hit
CGATCGAAAGGGAGTCGGGTTCAGATCCCCGAATCCGGAGTGAAAAAAAA1196130.2353878921402233No Hit
GTACAGTGAAACTGCGAATGGCTCAAAAAAAAAAAAAAAGCCTAAAGATC1149510.22621348506776692No Hit
GAGGCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1134150.22319077179807734No Hit
AAGGCGAGCTCAGGGAGGACAAAAAAAAAAAAAAGCCTAAAGATCGGAAG1055010.20761671397495No Hit
GAAAGGGAGTCGGGTTCAAAAAAAAAAAAAAGCCTAAAGATCGGAAGAGC949370.18682768859669413No Hit
GAGCTCTCGCTGGCCCTTGAAAATCCGGGGGAGAGGGAAAAAAAAAAAAA909950.1790701783694048No Hit
AAAGGAATTGACGGAAGGGCACCACCAAAAAAAAAAAAAAAGCCTAAAGA906360.17836369785910625No Hit
AAGAGGGACGGCCGGGGGCATTCGTAAAAAAAAAAAAAAAGCCTAAAGAT799260.15728735728724708No Hit
TGGGTTCGCCCCGAGAGAGGGGCCCGTGCCTTGGAAAGCGTCGCGAAAAA782320.15395371387653473No Hit
AGAGTGTTCAAAGCAGGCCCAAAAAAAAAAAAAAAGCCTAAAGATCGGAA780010.1534991261387103No Hit
AGTGCGGTAACGCGACCGATCCCGGAGAAGCCGGCGGGAGCCCCGGGGAG777360.15297762938319745No Hit
CCGTTGCCCTCGGCCGATCGAAAGGGAGTCGGGTTCAGATCCCCGAATCC701900.13812776328093326No Hit
AAGAGGTAAACGGGTGGGGTCCGCGCAAAAAAAAAAAAAAAGCCTAAAGA668840.1316218452668748No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA623040.12260880700178467No Hit
AAGAGGGCGTGAAACCGTTAAGAGGTAAAAAAAAAAAAAAAGCCTAAAGA614260.12088098001559493No Hit
AAGAGGGCGTGAAACCGTTAAGAGGTAAACGGGTGGGGTCCGCGCAAAAA604890.11903704620459286No Hit
AGAGGTCTTGGGGCAAAAAAAAAAAAAAAGCCTAAAGATCGGAAGAGCAC577440.11363512698239367No Hit
CCGAAGCGTTTACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCCCGAAAA542920.10684189377473186No Hit
GGGGAAAAAAAAAAAAAAAGCCTAAAGATCGGAAGAGCACACGTCTGAAC540840.10643256801946141Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GTACAGTGAAACTGCGAATGGCTCAAAAAAAAAAAAAAGCCTAAAGATCG509370.10023954805871063No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)