FastQCFastQC Report
Thu 14 Sept 2023
SRR14714424_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR14714424_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42207133
Total Bases3.5 Gbp
Sequences flagged as poor quality0
Sequence length84
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT8659782.0517337673705534No Hit
CCGTTGCCCTCGGCCGATCGAAAGGGAGTCGGGTTCAGATCCCCGAATCC3192730.7564432296313517No Hit
CCCCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTCAAAAAA3172020.7515364760738428No Hit
TACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGTC2673140.6333384454234312No Hit
GGGGCAAAAAAAAAAAAAAATGGTCAAGATCGGAAGAGCACACGTCTGAA1985960.4705271026108312Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CCTGTAATTGGAATGAGTCCACTTTAAATCCTTTAACGAGGATCCATTGG1575630.37330893808873494No Hit
GGGGAAAAAAAAAAAAAAATGGTCAAGATCGGAAGAGCACACGTCTGAAC1518650.3598088503192103Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGATCGAAAGGGAGTCGGGTTCAGATCCCCGAATCCGGAGTGAAAAAAAA1422560.33704255629018914No Hit
GTACAGTGAAACTGCGAATGGCTCAAAAAAAAAAAAAAATGGTCAAGATC1116480.26452400829973455No Hit
GGATGGTTTAGTGAGGCCCTCGGATCGGCAAAAAAAAAAAAAAATGGTCA1074270.2545233290306641No Hit
GGGGCAAAAAAAAAAAAAATGGTCAAGATCGGAAGAGCACACGTCTGAAC1071260.25381017943104545Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CTAAACCATTCGTAGACGACCTGCTTCTGGGTCGGGGTTTCGTACGTAGC1050640.24892474928349195No Hit
TTGGATAACTGTGGTAATTCTAGAGCTAATACATGCAAAAAAAAAAAAAA1047210.24811209043741492No Hit
GGGGAGAAAAAAAAAAAAAAATGGTCAAGATCGGAAGAGCACACGTCTGA969930.229802389089067Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAAAAAAAA964600.2285395693661543No Hit
CTAAGTCAGAATCCCGCCCAGGCGGAACGATACAAAAAAAAAAAAAAATG949120.22487194285382997No Hit
CCCCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTAAAAAAA934340.22137016508560295No Hit
CGGCGGCGTCCGGTGAGCTCTCGCTGGCCCTTGAAAATCCGGGGGAGAGG891580.21123917608902743No Hit
TGGGTTCGCCCCGAGAGAGGGGCCCGTGCCTTGGAAAGCGTCGCGAAAAA849340.2012313890166385No Hit
CCCCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAAAAAAAAAAAAA772240.18296433448820132No Hit
GGGGAAAAAAAAAAAAAATGGTCAAGATCGGAAGAGCACACGTCTGAACT771480.18278427013746704Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GGGGCAAAAAAAAAAAAATGGTCAAGATCGGAAGAGCACACGTCTGAACT762860.18074196131729678Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GGCGGCGTCCGGTGAGCTCTCGCTGGCCCTTGAAAATCCGGGGGAGAGGA712410.16878900540342315No Hit
GGTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGG708920.16796213095070922No Hit
AAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAATTCT665410.15765344687117222No Hit
CTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCAAAAAAAAAAAAA664670.1574781210559836No Hit
AAGAGGGCGTGAAACCGTTAAGAGGTAAAAAAAAAAAAAAATGGTCAAGA652960.15470370849401213No Hit
CCCTGTAATTGGAATGAGTCCACTTTAAATCCTTTAACGAGGATCCATTG643880.15255241335629216No Hit
CACGGGAAACCTCACCCGGCCCGGACACGGACAGGATTGACAAAAAAAAA598610.14182673814873897No Hit
TTTCGATGGTAGTCGCCGTGCCTACCATGGTGACCACGGGTGACGGGGAA595680.14113254269130293No Hit
TCGATGGTAGTCGCCGTGCCTACCATGGTGACCACGGGTGACGGGGAATC589630.1396991356887472No Hit
CCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAG563660.13354614728273537No Hit
AAGAGGGCGTGAAACCGTTAAGAGGTAAACGGGTGGGGTCCGCGCAGTCC547800.12978848859504385No Hit
GGATGGTTTAGTGAGGCCCTCGGATCGGCAAAAAAAAAAAAAATGGTCAA500490.11857948276183554No Hit
ACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCAAAAAAAAAAA498070.11800611996081325No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAAAAAAA492610.1167124997568539No Hit
GGCGGCGTCCGGTGAGCTCTCGCTGGCCCTTGAAAATCCGGGGGAGAGGG486600.11528856982538946No Hit
GTACAGTGAAACTGCGAATGGCTCAAAAAAAAAAAAAATGGTCAAGATCG466680.11056898842193334No Hit
GCCCGTCGCTACTACCGATTGGATGGTTTAGTGAGGCCCTCGGATCGGCC455370.10788934657087464No Hit
GGGAGAAAAAAAAAAAAAAATGGTCAAGATCGGAAGAGCACACGTCTGAA452450.10719752038121139Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGGGCAAAAAAAAAAAATGGTCAAGATCGGAAGAGCACACGTCTGAACTC436510.10342090755133734Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGTCG435550.1031934578451467No Hit
CCCCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGCCAAAAAA423770.10040246040876549No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)