FastQCFastQC Report
Thu 14 Sept 2023
SRR14714425_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR14714425_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences62997957
Total Bases5.2 Gbp
Sequences flagged as poor quality0
Sequence length84
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTGGGATTATGACTGAACGCCTCTAAGTCAGAATCCCGCCCAGGCGGA2573610.4085227716194035No Hit
AAAGAGTGTTCAAAAAAAAAAAAAAACGTGATAGATCGGAAGAGCACACG2434230.3863982446287901No Hit
AGAGTGGCGCAGCGGAAGCGTGCTGGGCCCAAAAAAAAAAAAAAACGTGA2278310.3616482356721505No Hit
AAAGAGTGTTTTAGATCAAAAAAAAAAAAAAACGTGATAGATCGGAAGAG2124590.33724744438934745No Hit
AAGGGACGAAAGCGTGGGGAGCAAAAAAAAAAAAAAACGTGATAGATCGG2079410.33007578325119336No Hit
AAGAGGGCGTGAAACCGTTAAAAAAAAAAAAAAACGTGATAGATCGGAAG2034350.32292317034979406No Hit
AATTATTCCCCATGAACGAGGAATTCCCAGTAAGTGCGGGTCAAAAAAAA1755380.2786407819542465No Hit
AAGGGACGAAAGCGTGGGGAGCAAACAAAAAAAAAAAAAAACGTGATAGA1603150.2544765062778147No Hit
TGTGGGATTATGACTGAACGCCTCTAAGTCAGAATCCCGCCCAGGCGGAA1576200.250198589773316No Hit
ATCCTTTAACGAGGATCCATTGGAGGGCAAGTCTGAAAAAAAAAAAAAAA1531640.24312534452506132No Hit
AAGGCGAGCTCAGGGAGGACAAAAAAAAAAAAAAACGTGATAGATCGGAA1454030.2308058974039428No Hit
GAGCTCTCGCTGGCCCTTGAAAATCCGGGGGAGAGGGAAAAAAAAAAAAA1431550.22723752771855762No Hit
GCCCGTCGCTACTACCGATTGGATGGTTTAGTGAGGCCCTCGGATCGGCC1321450.2097607704960972No Hit
AACGGCGAGTGAACAGGGAAGAGCCCAAAAAAAAAAAAAAACGTGATAGA1309030.20778927799198316No Hit
ATCCTTTAACGAGGATCCATTGGAGGGCAAGTCTGGTGCCAAAAAAAAAA1285310.20402407652679913No Hit
AAGAGGGCGTGAAACCGTTAAGAGGTAAAAAAAAAAAAAAACGTGATAGA1279290.20306848998293708No Hit
CCCCGAGAGAGGGGCCCGTGCCTTGGAAAGCGTCGCGAAAAAAAAAAAAA1207140.1916157376341585No Hit
TGGGATTATGACTGAACGCCTCTAAGTCAGAATCCCGCCCAGGCGGAACG1200670.19058872020246623No Hit
AGCGTTTACTTTGAAAAAATTAGAGTGTTCAAAAAAAAAAAAAAACGTGA1148950.18237893016117968No Hit
AACGGCGAGTGAACAGGGAAGAGCCAAAAAAAAAAAAAAACGTGATAGAT1148870.18236623133667654No Hit
AGGGCGGAGGGAAGCTCATCAAAAAAAAAAAAAAACGTGATAGATCGGAA1147230.18210590543436192No Hit
AATTATTCCCCATGAACGAGGAATTCCCAGTAAGTGCGGGTCATAAGCTT1107410.17578506553791895No Hit
ATCCTTTAACGAGGATCCATTGGAGGGCAAGTCTGGTGCCAGCAGCCGCG1103670.17519139549239668No Hit
ATTATTCCCCATGAACGAGGAATTCCCAGTAAGTGCGGGTCAAAAAAAAA1088090.1727182994204082No Hit
AAAGAGTGTTCAAAAAAAAAAAAAAAACGTGATAGATCGGAAGAGCACAC1008520.16008773109896246No Hit
AAAGAGTGTTCAAAAAAAAAAAAAACGTGATAGATCGGAAGAGCACACGT1002360.1591099216122199No Hit
CCGGTGAGCTCTCGCTGGCCCTTGAAAATCCGGGGGAGAGGAAAAAAAAA969490.15389229209448807No Hit
AGAGTGTTCAAAAAAAAAAAAAAACGTGATAGATCGGAAGAGCACACGTC969250.15385419562097863No Hit
CAGGGCGCCCTGGAATGGGTTCGCCCCGAGAGAGGGGCCCGTGCCTTGGA931300.14783019074729675No Hit
ATCCTTTAACGAGGATCCATTGGAGGGCAAGTCTGGTGCAAAAAAAAAAA920240.14607457825973627No Hit
ATCCTTTAACGAGGATCCATTGGAGGGCAAGTAAAAAAAAAAAAAAACGT862910.1369742831501663No Hit
AAAGGAATTGACGGAAGGGCACCACCAGGAGTGGAGCCTGCGGCTTAAAA860160.1365377610578705No Hit
AAGAGTGTTCAAAAAAAAAAAAAAACGTGATAGATCGGAAGAGCACACGT857720.13615044691052441No Hit
CCGGCCGAGGTGGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCA825730.13107250446232727No Hit
AGAGGAAGGTGGGGATGACGTCAAAAAAAAAAAAAAACGTGATAGATCGG821420.13038835529222004No Hit
ATCCTTTAACGAGGATCCATTGGAGGGCAAGTCAAAAAAAAAAAAAAACG818790.12997088143667895No Hit
AAGAGGGCGTGAAACCGTTAAAAAAAAAAAAAAAACGTGATAGATCGGAA815090.1293835608034083No Hit
AGCGTGCAGGATGAAGTTCTTCGGAATGTAAACTGCTGTTATAAGGGAAG776350.1232341550377578No Hit
GCCCCGAGAGAGGGGCCCGTGCCTTGGAAAGCGTCGCGAAAAAAAAAAAA771540.12247063821450592No Hit
AGAGTGGCGCAGCGGAAGCGTGCTGGGCCCAAAAAAAAAAAAAACGTGAT770100.1222420593734492No Hit
CGGAGGTTCGAAGACGATCAAAAAAAAAAAAAAACGTGATAGATCGGAAG763920.12126107518058087No Hit
ATCCTTTAACGAGGATCCATTGGAGGGCAAGTCTGGTGAAAAAAAAAAAA752000.11936895032961148No Hit
GAAAAAATTAGAGTGTTCAAAAAAAAAAAAAAACGTGATAGATCGGAAGA710210.11273540187977842No Hit
AAAGAGTGTTTTAGATCAAAAAAAAAAAAAACGTGATAGATCGGAAGAGC709830.11267508246338845No Hit
ATTATTCCCCATGAACGAGGAATTCCCAGTAAGTGCGGGTCATAAGCTTG707790.11235126243855813No Hit
AAGGGACGAAAGCGTGGGGAGCAAAAAAAAAAAAAAAACGTGATAGATCG696830.11061152348162656No Hit
AGGAGGTGTCAGAAAAGTTACCACAAAAAAAAAAAAAAACGTGATAGATC690420.10959403016831165No Hit
AGAGTTGCGCAGCGGAAGCGTGCTGGGCCCAAAAAAAAAAAAAAACGTGA660860.10490181451439766No Hit
AGTACCGTGAGGGAAAGGTGAAAAGCAAAAAAAAAAAAAAACGTGATAGA656480.10420655387285019No Hit
AGGGCGGAGGGAAGCTCATCAAAAAAAAAAAAAAAACGTGATAGATCGGA646520.10262555022220798No Hit
AAGAGGGCGTGAAACCGTTAAAAAAAAAAAAAACGTGATAGATCGGAAGA636410.10102073627562239No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)