FastQCFastQC Report
Thu 14 Sept 2023
SRR14714426_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR14714426_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40333916
Total Bases3.3 Gbp
Sequences flagged as poor quality0
Sequence length84
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AATTATTCCCCATGAACGAGGAATTCCCAGTAAGTGCGGGTCATAAGCTT1742230.43195161114532No Hit
GGGGAAAAAAAAAAAAAAAACATCGAGATCGGAAGAGCACACGTCTGAAC1594930.3954314775684067Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGGAGGTGGAGCACGAGCGCACGTGTTAGGACCCGAAAGATGGTGAACTA1492360.3700012664279858No Hit
ATTATTCCCCATGAACGAGGAATTCCCAGTAAGTGCGGGTCATAAGCTTG1404600.34824290306946637No Hit
GGGCCGCCGGTGAAATACCACTACTCTGATCGTTTTTTCACTGACCCGGT1206650.29916509966451066No Hit
AATTATTCCCCATGAACGAGGAATTCCCAGTAAGTGCGGGTCAAAAAAAA1117670.2771042613367867No Hit
GGGGCAAAAAAAAAAAAAAAACATCGAGATCGGAAGAGCACACGTCTGAA1046650.2594962512442382Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGGGTGACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACG950450.2356453561315494No Hit
GGGGAGGTGGAGCACGAGCGCACGTGTTAGGACCCGAAAGATGGTGAACT943660.23396190937671416No Hit
AAGAGGGCGTGAAACCGTTAAGAGGTAAACGGGTGGGGTCCGCGCAGTCC857620.21262998613871265No Hit
AGTGCGGTAACGCGACCGATCCCGGAGAAGCCGGCGGGAGCCCCGGGGAG851900.2112118248076879No Hit
CACGGGAAACCTCACCCGGCCCGGACACGGACAGGATTGACAAAAAAAAA844710.2094292059318019No Hit
GGGGCAAAAAAAAAAAAAAACATCGAGATCGGAAGAGCACACGTCTGAAC830690.20595322308897554Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CACGGGAAACCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATA799060.19811118761689292No Hit
GGGGAAAAAAAAAAAAAAACATCGAGATCGGAAGAGCACACGTCTGAACT796870.19756822025414045Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CCCCGAGAGAGGGGCCCGTGCCTTGGAAAGCGTCGCGAAAAAAAAAAAAA724670.17966765240449253No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAAAAAAAAAAA704420.17464706377630182No Hit
ATCCTTTAACGAGGATCCATTGGAGGGCAAGTCTGGTGCCAGCAGCCGCG677250.1679107974539343No Hit
TAGGGCGGAGGGAAGCTCATCAGTGGGGCCACGAGCTGAGTGCAAAAAAA650100.16117948973761934No Hit
GGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTACCAC641740.1591067924076601No Hit
GGGGCAAAAAAAAAAAAAACATCGAGATCGGAAGAGCACACGTCTGAACT616000.15272506641804878Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AAGAGGGCGTGAAACCGTTAAGAGGTAAACGGGTGGGGTCCGCGCAAAAA607930.1507242688758513No Hit
ACGGGAAACCTCACCCGGCCCGGACACGGACAGGATTGACAAAAAAAAAA595390.14761522288091244No Hit
ATTATTCCCCATGAACGAGGAATTCCCAGTAAGTGCGGGTCAAAAAAAAA576220.14286239897955855No Hit
GGGGAAAAAAAAAAAAAAAAACATCGAGATCGGAAGAGCACACGTCTGAA564900.14005582795382426Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATTATTCCCCATGAACGAGGAATTCCCAGTAAGTGCGGGTCATAAGCAAA545090.13514432865878928No Hit
GTGAAGGGCAGGGCGCCCTGGAATGGGTTCGCCCCGAGAGAGGGGCCCGT518860.12864111682089088No Hit
GAGGAATTCCCAGTAAGTGCGGGTCATAAGCTTGCGTTGATTAAGTCCCT514680.12760476815591126No Hit
CGGCCGGGCGCGACCCGCTCCGGGGACAGTGCCAGGTGGGGAGTTTGACT504270.12502381370556728No Hit
AAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAATTCT501830.12441886376715813No Hit
AAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCAAAAAAAAAAAAAAA499810.1239180445558522No Hit
GGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGGC450740.11175210460595991No Hit
GCGTCCAGTGCGGTAACGCGACCGATCCCGGAGAAGCCGGCGGGAGCCCC450400.11166780830306683No Hit
GGGTGACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGG449540.11145458824280788No Hit
CGCCGGCCGAGGTGGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCAC442140.10961990400337027No Hit
CAAAGCATCGCGAAGGCCCGCGGCGGGTGTTGACGCGATGTGATTTCTGC413340.10247951128772123No Hit
AATTATTCCCCATGAACGAGGAATTCCCAGTAAGTGCGGGTCATAAAAAA405230.10046879653341867No Hit
GAGAGAGTTCAAGAGGGCGTGAAACCGTTAAGAGGTAAACGGGTGGGGTC403680.1000845045643473No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)