FastQCFastQC Report
Thu 14 Sept 2023
SRR14824512.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR14824512.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences146573353
Total Bases5.2 Gbp
Sequences flagged as poor quality0
Sequence length10-150
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGACCTCAGATCAGACGTGGCGACCCGCTGA55054773.7561240752949137No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGA30849472.1047120345264942No Hit
GGGGGGCCCAAGTCCTTCTGATCGAGGC27110591.8496261049578362No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAA26889671.8345537882318896No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAA26042421.7767499662779769No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGC25489281.7390118652740378No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCG22944781.565412780043314No Hit
CCCTTGTAAATTTAACTGTTAGTCCAAAG21585141.4726510350077069No Hit
CGCCGCGACCGGCTCCGGGA14651520.9996032498485588No Hit
ATGGATTTTTGGAGCAGGG13238810.9032207921176505No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGC10733430.7322906776922815No Hit
CGCGACCGGCTCCGGGACGGCTGGG9353150.6381207640109045No Hit
CCCTTGTAAATTTAACTGTTAGTCCA8781160.5990966175140989No Hit
CGCCGCGACCGGCTCCGGGACGGCTGGG7937250.5415206678119726No Hit
CCCTTGTAAATTTAACTGTTAGTCCAAAGAG7151930.4879420340476212No Hit
CCCTTGTAAATTTAACTGTTAGTCCAA7100910.4844611830637456No Hit
GGGGGGCCCAAGTCCTTCTGATCGAGGCC6804180.46421671202404713No Hit
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGG6580830.4489786080011419No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAAT6511530.44425059990269855No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCC6383430.4355109485692123No Hit
CGCGACCGGCTCCGGGACGGCTGG6255100.42675560543395635No Hit
AGCGGAGGAGAAGAAACTAACCAGGATTCCCT6250890.4264683772363453No Hit
CGACCTCAGATCAGACGTGGCGACCCGCTGA6070890.4141878367209079No Hit
ATGGATTTTTGGAGCAGG6059260.41339437735316054No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTG5273850.3598096033185514No Hit
CCCCTTGTAAATTTAACTGTTAGTCCAAAG5196480.3545310176536659No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAT4938180.33690844201401327No Hit
CCCTTGTAAATTTAACTGTTAGTCCAAAGA4746430.3238262551038182No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAATT4626970.31567606971507295No Hit
TTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGG4540400.30976981197939846No Hit
AGGGCTTCTGTCGTGAGTGGCACACGTAGG4414840.30120345271763005No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATT4214960.28756659472748775No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGC3919880.2674346953091808No Hit
CGGCTCCGGGA3858890.26327363883120014No Hit
TGGAGCACGAGCGCACGTGTTAGGACCCGA3846880.2624542538779201No Hit
GATTTATAGGTAGAGGCGAC3812720.2601236801889904No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGC3739790.25514801452348573No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTG3616140.24671196544163113No Hit
CTAACCAGGATTCCCTCAGTAACG3527500.2406644814900291No Hit
CCCTTGTAAATTTAACTGTTAGTCCAAA3500870.23884764374599524No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCC3415390.2330157515056642No Hit
AGCACGAGCGCACGTGTTAGGACCCGA3116700.21263755902479767No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACAC3103230.2117185652428924No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCC3099930.2114934220001094No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGC2704350.18450488746068325No Hit
AGTGAACAGGGAAGAGCCCAGCGCCGAATCCCC2601890.17751453089839597No Hit
CTAAACCATTCGTAGACGACCTGCTT2574870.17567108531657866No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCC2511120.17132172721736125No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGC2457120.16763756506273006No Hit
ATTCCCCATGAACGTACACTGACTGGCTCAGCG2434780.1661134135343141No Hit
CAAATAAAGTATAGGCGATAGAAATTGAAACC2338060.1595146697640191No Hit
AACTAACCAGGATTCCCTCAGTAACG2281540.15565858004217178No Hit
TCCCACATGGTCTAGCGGTTAGGATTCCTG2244830.1531540320292734No Hit
GCACGAGCGCACGTGTTAGGACCCGA2224420.151761555185273No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCT2082110.14205242340331806No Hit
ATTCCCCATGAACTACACTGACTGGCTCAGCG2046960.1396543067415535No Hit
GGAGCACGAGCGCACGTGTTAGGACCCGA1966770.13418332594192617No Hit
AGGGCTTCTGTCGTGAGTGGCACACGTA1885370.12862979261994506No Hit
AGACCCTGTTGAGCTTGACTCTAGTCTGGCACG1866970.12737444847836701No Hit
ACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGA1845110.12588304505799222No Hit
AGCGGAGGAGAAGAAACTAACCAGGATTCCC1808050.12335461821631384No Hit
CACTCGCCGAATCCCGGGGCCGAGGGA1790300.12214362047104155No Hit
ACGGTGGCCATGGAAGTCGGAATCCGCT1788380.12201262803887689No Hit
CATAATTTGTGGTAGTGG1766110.12049325227621695No Hit
ATCCAAGATTGTCCGCACCAGTTCT1746630.11916422489154628No Hit
CATAATTTGTGGTAGTGGG1710460.11669651850019425No Hit
CGACCGGCTCCGGGACGGCTGGG1694620.11561583093483575No Hit
CGACCGGCTCCGGGACGGCTGG1671730.11405415553262263No Hit
TAAGTGGGAGGCC1661500.11335621147999528No Hit
GAGCACGAGCGCACGTGTTAGGACCCGA1641650.11200194076204288No Hit
CGCCGCGACCGGCTCCGGGACG1637190.11169765625816037No Hit
CACGAGCGCACGTGTTAGGACCCGA1567000.10690892770939067No Hit
CTCCATCTAAGGCTAAATACCGGCACGAGACCGA1522730.10388859699484393No Hit
CGCGACCTCAGATCAGACGT1522630.10388177447233536No Hit
CGACCTCAGATCAGACGTGGCGACCCGCTGAA1520120.10371052915737008No Hit
CGGGACATGTGGCGTACGGAAGACCCG1511060.10309240861809307No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)