FastQCFastQC Report
Thu 14 Sept 2023
SRR15049345_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR15049345_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31841660
Total Bases1.6 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTAGGGGTATGATTCTCGCTTAGATCGGAAGAGCACACGTCTGAACTC21651406.7997083066649155Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTC13578744.264457317865966No Hit
GTATAGTGGTGAGTATCCCCGCAGATCGGAAGAGCACACGTCTGAACTCC10847793.4067916057140235Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGGGGGCGGGCGGGCGTCCCGCGCGTGTGGGGGGAAGATCGGAAGAGCAC7530202.3648892677077766No Hit
ATAGCTCAGTCGGTAGAGCATCAGAAGATCGGAAGAGCACACGTCTGAAC7283842.2875189296035447Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ATCGTATAGTGGTTAGTACTCTGCGTAGATCGGAAGAGCACACGTCTGAA6750112.1198988997432924Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGAAGATCGGAAGAGCACACGT6515932.046353739095261No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGAAGATCGGAAGAGCACACGT6348531.9937811031208799No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTC6120121.9220480339278794No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATG5086801.5975297770279566TruSeq Adapter, Index 7 (100% over 49bp)
GTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTCTGAACT5054011.5872319470781362Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GTCTAGCGGTTAGGATTCCTGGTAGATCGGAAGAGCACACGTCTGAACTC4844411.5214062332177405Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GCGCCGCTGGTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACG4608551.4473334618860951No Hit
GTCTAGGGGTATGATTCTCGCTAGATCGGAAGAGCACACGTCTGAACTCC4160101.3064959552988131Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CTGGTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACGTCTGAA3106380.9755709972407217Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTCTGAAC2763270.8678159367319418Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCATTGGTAGTTCAATGGTAGAATTCTCGCAGATCGGAAGAGCACACGTC2503000.7860771077889784No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGT2363660.7423168264468624No Hit
TTAGTATAGTGGTGAGTATCCCCGCAGATCGGAAGAGCACACGTCTGAAC2322050.7292490404080693Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACG2309050.7251663386896287No Hit
CTAGCTCAGTCGGTAGAGCATGAGAAGATCGGAAGAGCACACGTCTGAAC2184270.6859786832721662Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACGTCTGAACTC2068240.6495390001651924Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGTCTGAAC2068160.6495138758469251Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACGTCTGAACTC1999520.6279572107735589Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GTCTAGCGGTTAGGATTCCTGGTTAGATCGGAAGAGCACACGTCTGAACT1947770.611704917394382Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GTCTAGGGGTATGATTCTCGCAGATCGGAAGAGCACACGTCTGAACTCCA1812450.5692071330451993Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCCTCGTTAGTATAGTGGTGAGTATCCCCGCAGATCGGAAGAGCACACGT1705680.5356755897776687No Hit
ATGGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTCTG1586760.49832829067328777Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTCTGAACTCCA1404920.44122071525165457Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACGTCTGAA1401590.4401749155037772Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCAGATCGGAAGAGCAC1319890.4145167054732699No Hit
CCCAGGCGGCCCGGGTTCGACTCCCGGTGTGGGAACAGATCGGAAGAGCA1251620.3930762403718901No Hit
GTAGTTCAATGGTAGAATTCTCGCAGATCGGAAGAGCACACGTCTGAACT1170550.3676158843477381Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCAGATCGGAAGAGCACACGT1122950.3526669149786789No Hit
TAGTATAGTGGTGAGTATCCCCGCAGATCGGAAGAGCACACGTCTGAACT1100400.3455849977670762Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTGAACT1085030.3407579881199661Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GCCCGGATAGCTCAGTCGGTAGAGCATCAGAGATCGGAAGAGCACACGTC1079430.3389992858412533No Hit
GTATAGTGGTTAGTATCCCCGCAGATCGGAAGAGCACACGTCTGAACTCC1058620.33246382255196494Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTCTGAACTCC1022040.3209757280242299Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CTCAGTCGGTAGAGCATCAGAAGATCGGAAGAGCACACGTCTGAACTCCA1017760.3196315769969279Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GTATAGTGGTGAGTATCCCCGCCAGATCGGAAGAGCACACGTCTGAACTC1001460.3145124971499601Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ATTGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTCTG995540.312653297598178Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTCTAGGGGTATGATTCTCGGTAGATCGGAAGAGCACACGTCTGAACTCC962450.30226125145485505Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTCTGAAC931630.2925821078423675Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ATCGTATAGTGGTTAGTACTCTGCGTTAGATCGGAAGAGCACACGTCTGA928410.29157085403210764Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT916610.287865017087677No Hit
ATAGCTCAGTCGGTAGAGCATCAGAGATCGGAAGAGCACACGTCTGAACT908670.2853714284996448Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GTATAGTGGTGAGTATCCCCGAGATCGGAAGAGCACACGTCTGAACTCCA876110.2751458309648429Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GTCTAGCGGTTAGGATTCCTGGAGATCGGAAGAGCACACGTCTGAACTCC842090.2644617146216623Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCCGTGATCGTATAGTGGTTAGTACTCTGCGTAGATCGGAAGAGCACACG839720.26371740669299276No Hit
ATCGTATAGGGGTTAGTACTCTGCGTAGATCGGAAGAGCACACGTCTGAA786710.24706940530110555Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CCGTGATCGTATAGTGGTTAGTACTCTGCGTAGATCGGAAGAGCACACGT750850.23580742963777643No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGAAGATCGGAAGAGCACACGT748700.23513221358434203No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGAGATCGGAAGAGCACACGTCT721440.2265711021347505Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTCGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTC689500.2165402180665204No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGAGATCGGAAGAGCACACGTC683130.21453969422448455No Hit
GCGCAGCGGAAGCGTGCTGGGCAGATCGGAAGAGCACACGTCTGAACTCC663040.20823034979960214Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCGTTGGTGGTATAGTGGTGAGCATAGCTGCAGATCGGAAGAGCACACGT612600.1923894671320528No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCAGATCGGAAGAGCACACGTC611800.19213822394937952No Hit
GTTTCCGTAGTGTAGTGGTTATCAGATCGGAAGAGCACACGTCTGAACTC579490.18199113990916302Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCAGATCGGAAGAGCACA573010.17995607012950957No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAAGATCGGAAGAGCACA572920.17992780527145885No Hit
GTCTAGCGGTTAGGATTCCTGGTTTAGATCGGAAGAGCACACGTCTGAAC566810.17800893546379176Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGCAGCAACCTCGGTTCGAATCCGAGTCACGGCAGATCGGAAGAGCACAC562240.17657370878277073No Hit
GTATAGTGGTGAGTATCCCCGCCTAGATCGGAAGAGCACACGTCTGAACT549340.17252241246216435Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGAGATCGGAAGAGCACACGTCT540810.16984353202691066Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCCGAGATCGGAAGAGCAC530960.1667501003402461No Hit
CCAGTGCGCCCCGGGCGTCGTCGCGCAGATCGGAAGAGCACACGTCTGAA505110.1586318050001162Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GTATAGTGGTGAGCATAGCTGCAGATCGGAAGAGCACACGTCTGAACTCC476490.14964358013998014Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CCCAGGCGGCCCGGGTTCGACTCCCGGTGTGGGAAAGATCGGAAGAGCAC475800.14942688289492445No Hit
CATTGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTCT469910.14757710496249254Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGAGCAGTGGCTGGTTGAGATTTAAAGATCGGAAGAGCACACGTCTGAAC458890.1441162301211683Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGGGAAGATCGGAAGAGCACAC450740.1415566901976844No Hit
AGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCAGATCGGAAGAGCACAC447150.14042923641543814No Hit
AGTATAGTGGTGAGTATCCCCGCAGATCGGAAGAGCACACGTCTGAACTC442080.13883698274524633Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AACCTCGGTTCGAATCCGAGTCACGGCAGATCGGAAGAGCACACGTCTGA438590.1377409343608342Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTCTGAAC424510.13331905434578475Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTAGTATAGTGGTGAGTATCCCCGAGATCGGAAGAGCACACGTCTGAACT410750.12899767160380457Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATAGCTCAGTCGGTAGAGCATCAGACAGATCGGAAGAGCACACGTCTGAA409200.1285108879373751Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT404850.12714475313158924No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACGT396090.124393640281317No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTC385080.12093590597977617No Hit
CCCAGGCGGCCCGGGTTCGACTCCCGGTATGGGAACAGATCGGAAGAGCA381750.1198901062318987No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGGGAACAGATCGGAAGAGCAC381590.11983985759536406No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTATGGGAAGATCGGAAGAGCACAC381470.11980217111796307No Hit
TCCTCGTTAGTATAGTGGTGAGTATCCCCGAGATCGGAAGAGCACACGTC371390.11663650701627994No Hit
GTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTCTGAACTCC368520.11573517209843959Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCGTATAGTGGTTAGTACTCTGCGTAGATCGGAAGAGCACACGTCTGAAC354900.11145775691342726Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCGCCGCTGGTGTAGTGGTATCATGCAAGAAGATCGGAAGAGCACACGTC349900.10988748702171934No Hit
GTGGTATAGTGGTGAGCATAGCTGCAGATCGGAAGAGCACACGTCTGAAC333900.10486262336825403Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CTCAGTCGGTAGAGCATGAGAAGATCGGAAGAGCACACGTCTGAACTCCA332320.10436641808247434Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GTCTAGTGGCTAGGATTCGGCGCAGATCGGAAGAGCACACGTCTGAACTC331850.10421881271265379Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGCGAGAGGTCCCGGGTTCAAATCCCGGACGAGCAGATCGGAAGAGCACA328650.10321383998196075No Hit
CATGGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTCT323480.10159018091393476Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGTTCAATGGTAGAATTCTCGCAGATCGGAAGAGCACACGTCTGAACTCC321700.10103116483248674Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCATTTGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTC320380.10061661358107586No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)