FastQCFastQC Report
Mon 25 Sept 2023
SRR1506632.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1506632.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences85310273
Total Bases4.3 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGAGGTGCACAATCGACCGATCCTGAAGATCGGAAGAGCACACGTCTG41797644.899484965896194Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCGATATAGTGTAACGGCTATCACATCACGAGATCGGAAGAGCACACGT41643574.881425007279018No Hit
TCCGTGATAGTTTAATGGTCAGAATGGGCAGATCGGAAGAGCACACGTCT33657963.9453583743660037Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGCCAGATCGGGGTTCAATTCCCCGTCGCGGAAGATCGGAAGAGCACACG32087513.7612715176752514No Hit
TCCGTGATAGTTTAATGGTCAGAATGGGCGAGATCGGAAGAGCACACGTC19988972.3430906146555177No Hit
AAGAGGTGCACAATCGACCGATCCTGAAGATCGGAAGAGCACACGTCTGA18858522.210580195892703Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCCGATATAGTGTAACGGCTATCACATCACAGATCGGAAGAGCACACGTC16330101.9142008841068883No Hit
TGCCAGATCGGGGTTCAATTCCCCGTCGCGGAGAGATCGGAAGAGCACAC13501851.5826757464484964No Hit
TTTCCACGTTCTAGCATTCAAGGTCCCAAGATCGGAAGAGCACACGTCTG11280331.3222710001174185Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTTTCCACGTTCTAGCATTCAAGGTCCCAAGATCGGAAGAGCACACGTCT9622141.1278993328271263Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCCAGATCGGGGTTCAATTCCCCGTCGCGGAGCAGATCGGAAGAGCACAC8867421.0394316754794584No Hit
GCCAGATCGGGGTTCAATTCCCCGTCGCGGAGAGATCGGAAGAGCACACG8834891.035618535648104No Hit
TCCGATATAGTGTAACGGCTATCACATCAAGATCGGAAGAGCACACGTCT7755930.9091437323146299Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGCGCAGGTTCGAGTCCTGCAGTTGTCGCAGATCGGAAGAGCACACGTCT7658040.8976691470674347Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCTCGTATGGCGCAGTGGTAGCGCAGCAGAAGATCGGAAGAGCACACGTC6316770.7404465813865113No Hit
TCCGATGTAGTGTAACGGCTATCACATCACGAGATCGGAAGAGCACACGT5526780.6478446036622107No Hit
CGTGGGTTAGTCGATCCTAAGAGATGGGAGATCGGAAGAGCACACGTCTG5404040.633457121863858Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCCAGATCGGGGTTCAATTCCCCGTCGCGGAAGATCGGAAGAGCACACGT5388400.631623813933874No Hit
TGCCAGATCGGGGTTCAATTCCCCGTCGCGGAGATCGGAAGAGCACACGT5095970.5973454099719033No Hit
TCCGATATAGTGTAACGGCTATCACATCAGATCGGAAGAGCACACGTCTG4810690.5639051231262617Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGTGGGGACACGGGTTCGATTCTCGTACCGCAGATCGGAAGAGCACACGT4640520.5439579357576314No Hit
CGCGCAGGTTCGAGTCCTGCAGTTGTCGCCAGATCGGAAGAGCACACGTC4590220.5380618111490512No Hit
GGCGGTTAGTGTAGTGGTTATCATCCCACCAGATCGGAAGAGCACACGTC4559670.5344807652883727No Hit
CCGATATAGTGTAACGGCTATCACATCACGAGATCGGAAGAGCACACGTC4529450.5309384017561403No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCAGATCGGAAGAGCACACGTCTG4038720.4734154349734645Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCAGGGGGCATGCCTGTTTGAGCGTCATTAGATCGGAAGAGCACACGTC3903660.45758381291312944No Hit
CGTTGGGCCCCCGGTTCGATTCCGGGCTTGCGCAGATCGGAAGAGCACAC3854890.45186703364552594No Hit
TGGGGACACGGGTTCGATTCTCGTACCGCTAGATCGGAAGAGCACACGTC3815350.447232187382638No Hit
TTTCCACGTTCTAGCATTCAAGGTCCCAGATCGGAAGAGCACACGTCTGA3664140.42950747561199343Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGGCGGTTAGTGTAGTGGTTATCATCCCAGATCGGAAGAGCACACGTCTG3379180.3961046989030266Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCTCTGCTAGGCGGACTACTTGCGTGCCTAGATCGGAAGAGCACACGTCT3042550.3566452073128403Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGCTTGGTGGGGCTTGCTCTGCTAGGCGAGATCGGAAGAGCACACGTCTG2960210.34699338026968923Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCGATATAGTGTAACGGCTATCACATCACGCAGATCGGAAGAGCACACG2778000.3256348740086672No Hit
TCCAACGGGGCCTTTCCTTCTGGCTAACCTAGATCGGAAGAGCACACGTC2753050.3227102555397988No Hit
TCCAACGGGGCCTTTCCTTCTGGCTAACCAGATCGGAAGAGCACACGTCT2613730.3063792797849797Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CAAGAGGTGCACAATCGACCGATCCTGATAGATCGGAAGAGCACACGTCT2587120.30326007748211053Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CAAGAGGTGCACAATCGACCGATCCTGAGATCGGAAGAGCACACGTCTGA2572200.30151116735964495Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GACGTGGGTTAGTCGATCCTAAGAGATGGGAGATCGGAAGAGCACACGTC2571990.30148655133245206No Hit
TCCGTGATAGTTTAATGGTCAGAATGGGAGATCGGAAGAGCACACGTCTG2437210.2856877506417076Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGATATAGTGTAACGGCTATCACATCACGAGATCGGAAGAGCACACGTCT2412830.28282994710379133Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCGATGTAGTGTAACGGCTATCACATCACAGATCGGAAGAGCACACGTC2340590.2743620337494407No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCAGATCGGAAGAGCACACGTCTGA2332280.27338794238766534Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATG2328740.2729729865006996TruSeq Adapter, Index 9 (100% over 49bp)
TGTCCGCGCGAGTTCGAACCTCGCATCCTAGATCGGAAGAGCACACGTCT2328250.27291554910391624Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGTGGAGACCGGGGTTCGACTCCCCGTATCGGAGATCGGAAGAGCACACG2214140.25953966880401375No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATTTAGATCGGAAGAGCACACGT2025070.23737703898802434No Hit
CGTTGGGCCCCCGGTTCGATTCCGGGCTTGCGAGATCGGAAGAGCACACG1940820.22750132331659517No Hit
CGTGGGTTAGTCGATCCTAAGAGATGGGGAGATCGGAAGAGCACACGTCT1829680.2144735839727063Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGCGGTTAGTGTAGTGGTTATCATCCCACCAGATCGGAAGAGCACACGT1813160.21253712316686643No Hit
CGTGATAGTTTAATGGTCAGAATGGGCGAGATCGGAAGAGCACACGTCTG1806500.2117564434473208Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGCTGGCTCCGGTGCGCTTGTGACGGCCAGATCGGAAGAGCACACGTCTG1797780.21073429222293075Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGGTGGGGACACGGGTTCGATTCTCGTACCGCAGATCGGAAGAGCACACG1775500.20812264895694335No Hit
CGATGAAACCCTGGTTCGATTCTAGGAGAAGATCGGAAGAGCACACGTCT1767590.20719544526601152Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGTCCGCGCGAGTTCGAACCTCGCATCCTTAGATCGGAAGAGCACACGTC1763680.20673711828351551No Hit
ACGTGGGTTAGTCGATCCTAAGAGATGGGAGATCGGAAGAGCACACGTCT1709110.20034046778868003Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGAGGTGCACAATCGACCGATCCTGAAGATCGGAAGAGCACACGTCTGAA1694600.19863961752882914Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CCAGATCGGGGTTCAATTCCCCGTCGCGGAGCAGATCGGAAGAGCACACG1633310.1914552541638215No Hit
GTGATAGTTTAATGGTCAGAATGGGCGAGATCGGAAGAGCACACGTCTGA1618450.1897133771919825Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGCCCGCGCTGGTTCAAATCCTGCTGGTGAGATCGGAAGAGCACACGTCT1606490.18831143583375945Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAGAGGTGCACAATCGACCGATCCTGAGATCGGAAGAGCACACGTCTGAA1582260.18547121517240955Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGTTGCGGCCATATCTACCAGAAAGCACCAGATCGGAAGAGCACACGTCT1543510.18092897205943767Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTCCACGTTCTAGCATTCAAGGTCCCAAGATCGGAAGAGCACACGTCTGA1541820.18073087165012353Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCAACGGGGCCTTTCCTTCTGGCTAACCTAGATCGGAAGAGCACACGTCT1476580.17308349253553557Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCTCGTGTAGCTCAGTGGTTAGAGCTTCGTGAGATCGGAAGAGCACACGT1444840.1693629558540974No Hit
TTTCCACGTTCTAGCATTCAAGGTCCAGATCGGAAGAGCACACGTCTGAA1432590.16792702093451276Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GTCTAGTCGGTTATGGCATCTGCAGATCGGAAGAGCACACGTCTGAACTC1432550.1679223321674284Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGGGGACACGGGTTCGATTCTCGTACCGCAGATCGGAAGAGCACACGTCT1429410.16755426395130632Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCGATATAGTGTAACGGCTATCACATCACAGATCGGAAGAGCACACGTCT1405610.16476444753611325Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGTGATAGTTTAATGGTCAGAATGGGCAGATCGGAAGAGCACACGTCTGA1398390.16391812507738662Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTGCTGGCTCCGGTGCGCTTGTGACGGCCAGATCGGAAGAGCACACGTCT1388430.16275062207338148Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAGAGGTGCACAATCGACCGATCCTGATAGATCGGAAGAGCACACGTCTG1371150.16072507469293879Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGGCGTGTGGTCTAGAGGTATGATTCTCGCAGATCGGAAGAGCACACGTC1344390.15758828951350326No Hit
GTTCCAATAGTGTAGCGGCTATCACGTTGCCAGATCGGAAGAGCACACGT1276110.14958456410050405No Hit
TGCCCGCGCAGGTTCGAGTCCTGCAGTTGTCAGATCGGAAGAGCACACGT1264330.14820372219416061No Hit
CCGTGATAGTTTAATGGTCAGAATGGGCGAGATCGGAAGAGCACACGTCT1259190.1476012156238206Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGTGCCCGAGTTGTAATTTGGAGAGGGCAAGATCGGAAGAGCACACGTCT1247980.14628718864842924Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGGTGCCCGAGTTGTAATTTGGAGAGGGCAAGATCGGAAGAGCACACGTC1229990.1441784156522392No Hit
TACGCGAAACTCAGGTGCTGCAATCTAGATCGGAAGAGCACACGTCTGAA1229120.14407643496815442Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GTCTAGTCGGTTATGGCATCTGCTAGATCGGAAGAGCACACGTCTGAACT1209290.1417519786860839Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CCAGATCGGGGTTCAATTCCCCGTCGCGGAGAGATCGGAAGAGCACACGT1198060.14043560732715038No Hit
CGATGAAACCCTGGTTCGATTCTAGGAGATGAGATCGGAAGAGCACACGT1185090.1389152746000473No Hit
CAGATCGGGGTTCAATTCCCCGTCGCGGAGCAGATCGGAAGAGCACACGT1184720.13887190350451697No Hit
AGATCGGGGTTCAATTCCCCGTCGCGGAGCAGATCGGAAGAGCACACGTC1166910.13678422996020656No Hit
GGGCGGTTAGTGTAGTGGTTATCATCCCAAGATCGGAAGAGCACACGTCT1129340.13238030547622323Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGATCGGGGTTCAATTCCCCGTCGCGGAAGATCGGAAGAGCACACGTCTG1098550.12877112701303864Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCTCTGCTAGGCGGACTACTTGCGTGCCAGATCGGAAGAGCACACGTCTG1088060.12754149784516572Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGTGGGTTAGTCGATCCTAAGAGATGGAGATCGGAAGAGCACACGTCTGA1079490.12653692949734202Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTTCCAATAGTGTAGCGGCTATCACGTTGCAGATCGGAAGAGCACACGTC1079210.1265041081277515No Hit
TGAAACCCTGGTTCGATTCTAGGAGATGGCAGATCGGAAGAGCACACGTC1049810.12305786432074833No Hit
CCGTGATAGTTTAATGGTCAGAATGGGCAGATCGGAAGAGCACACGTCTG1037260.12158676364803099Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGGGTCTTATGGCTGGAAGAGGCCAGCACAGATCGGAAGAGCACACGTCT1022490.11985543640213178Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGCCAGATCGGGGTTCAATTCCCCGTCGCGGAGCAGATCGGAAGAGCACA1009170.11829407696304056No Hit
TGAACTTAGTACGAGAGGAACAGTTCAAGATCGGAAGAGCACACGTCTGA943350.11057871072572935Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCGCGCAGGTTCGAGTCCTGCAGTTGTCGCAGATCGGAAGAGCACACGTC924090.10832106937461096No Hit
GACGTGGGTTAGTCGATCCTAAGAGATGGAGATCGGAAGAGCACACGTCT919300.10775958951625908Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GATATAGTGTAACGGCTATCACATCACGAGATCGGAAGAGCACACGTCTG904050.10597199706534757Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATTACCCAATCCTAATTCAGGGAGGTAGTGAGATCGGAAGAGCACACGTC882570.10345412914104729No Hit
CTGCTTGGTGGGGCTTGCTCTGCTAGGCGGAGATCGGAAGAGCACACGTC881970.10338379763478191No Hit
TGCTCTGCTAGGCGGACTACTTGCGTGCCTAGATCGGAAGAGCACACGTC870190.10200295572843847No Hit
TGACGGCCCGTGAAAATCCACAGGAAGGAAGATCGGAAGAGCACACGTCT863340.10120000436524217Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGGGGTCTTATGGCTGGAAGAGGCCAGCACAGATCGGAAGAGCACACGTC859330.10072995546503527No Hit
GTGATAGTTTAATGGTCAGAATGGGCAGATCGGAAGAGCACACGTCTGAA854740.10019191944210518Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)