FastQCFastQC Report
Mon 25 Sept 2023
SRR1506633.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1506633.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36894154
Total Bases1.8 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGGTGCACAATCGACCGATCCTGAAGATCGGAAGAGCACACGTCTGA385588210.451200480162793Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CAAGAGGTGCACAATCGACCGATCCTGAAGATCGGAAGAGCACACGTCTG368950610.000245567360075Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCGATATAGTGTAACGGCTATCACATCACGAGATCGGAAGAGCACACGTC13362263.6217824645064365No Hit
TCCGATATAGTGTAACGGCTATCACATCACGAGATCGGAAGAGCACACGT12151093.293500103024452No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATG7059411.9134223812260338TruSeq Adapter, Index 11 (100% over 49bp)
CGTGGAGACCGGGGTTCGACTCCCCGTATCGGAGATCGGAAGAGCACACG4977901.3492381475937893No Hit
CGATATAGTGTAACGGCTATCACATCACGAGATCGGAAGAGCACACGTCT4025711.0911511888848298Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCGTGATAGTTTAATGGTCAGAATGGGCAGATCGGAAGAGCACACGTCTG4024471.0908150922772208Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCGTGATAGTTTAATGGTCAGAATGGGCAGATCGGAAGAGCACACGTCT3891461.0547633101981413Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGAGGTGCACAATCGACCGATCCTGAAGATCGGAAGAGCACACGTCTGAA2909550.7886208747326203Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTTCCACGTTCTAGCATTCAAGGTCCCAAGATCGGAAGAGCACACGTCTG2856760.7743123748006256Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGCCAGATCGGGGTTCAATTCCCCGTCGCGGAAGATCGGAAGAGCACACG2638190.7150699267965326No Hit
TGAATGGCTTAGTGAGGCCTCAGGATCTGCTAGATCGGAAGAGCACACGT2575260.6980130239603813No Hit
CCGATATAGTGTAACGGCTATCACATCACAGATCGGAAGAGCACACGTCT2568510.6961834658141233Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCGATGTAGTGTAACGGCTATCACATCACGAGATCGGAAGAGCACACGTC2347480.6362742455078385No Hit
CAAGAGGTGCACAATCGACCGATCCTGAGATCGGAAGAGCACACGTCTGA2240120.6071747843845396Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGTTGGGCCCCCGGTTCGATTCCGGGCTTGCGCAGATCGGAAGAGCACAC2179690.5907954956766321No Hit
GAATGGCTTAGTGAGGCCTCAGGATCTGCTAGATCGGAAGAGCACACGTC2098170.5686998541828605No Hit
TCCGATATAGTGTAACGGCTATCACATCACAGATCGGAAGAGCACACGTC1852480.5021066481155795No Hit
TCCGATGTAGTGTAACGGCTATCACATCACGAGATCGGAAGAGCACACGT1652040.4477782577695101No Hit
GGGCGTGCTTGTGGACTGCTTGGTGGGGCAGATCGGAAGAGCACACGTCT1527700.4140764414871798Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAGAGGTGCACAATCGACCGATCCTGAGATCGGAAGAGCACACGTCTGAA1445540.39180733077657776Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCCAGATCGGGGTTCAATTCCCCGTCGCGGAAGATCGGAAGAGCACACGT1427130.38681738033619095No Hit
TTTCCACGTTCTAGCATTCAAGGTCCCAGATCGGAAGAGCACACGTCTGA1340430.3633177223686983Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGTGGAGACCGGGGTTCGACTCCCCGTATCGGAAGATCGGAAGAGCACAC1335550.36199501959036656No Hit
GCTCGTATGGCGCAGTGGTAGCGCAGCAGAAGATCGGAAGAGCACACGTC1176240.31881473688216294No Hit
GTGGAGACCGGGGTTCGACTCCCCGTATCGGAGATCGGAAGAGCACACGT1082930.29352346715959393No Hit
GATATAGTGTAACGGCTATCACATCACGAGATCGGAAGAGCACACGTCTG1025480.2779518944925529Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGTTTCTTTACTTATTCAATGAAGCGGAGAGATCGGAAGAGCACACGTCT1014750.2750435746541308Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCGATATAGTGTAACGGCTATCACATCACGCAGATCGGAAGAGCACACGT992600.26903991347789136No Hit
CGTTGGGCCCCCGGTTCGATTCCGGGCTTGCGAGATCGGAAGAGCACACG951190.2578159130576622No Hit
GGTCAGAATGGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGC937800.25418661178678875TruSeq Adapter, Index 11 (100% over 36bp)
TGCCAGATCGGGGTTCAATTCCCCGTCGCGGAGATCGGAAGAGCACACGT936480.2538288315270761No Hit
CGGGGCCTTTCCTTCTGGCTAACCTTGAGTAGATCGGAAGAGCACACGTC846970.2295675352794375No Hit
AGCGGAGAATTTGGACAAACTTGGTCATTAGATCGGAAGAGCACACGTCT729230.1976546202956707Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATAGTGTAACGGCTATCACATCACGAGATCGGAAGAGCACACGTCTGAAC705750.19129046840320557Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGTGATAGTTTAATGGTCAGAATGGGCAGATCGGAAGAGCACACGTCTGA687550.1863574375495912Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGGGCGTGCTTGTGGACTGCTTGGTGGGGCAGATCGGAAGAGCACACGTC673410.18252485204024463No Hit
CGATATAGTGTAACGGCTATCACATCACAGATCGGAAGAGCACACGTCTG659710.178811526617469Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCGTGATAGTTTAATGGTCAGAATGGGCGAGATCGGAAGAGCACACGTCT650110.17620948836501305Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGCGTGCTTGTGGACTGCTTGGTGGGGAGATCGGAAGAGCACACGTCTG629550.1706367897743366Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCGTGATAGTTTAATGGTCAGAATGGGCGAGATCGGAAGAGCACACGTC609800.1652836381612111No Hit
TCCGATATAGTGTAACGGCTATCACATCACGCAGATCGGAAGAGCACACG564620.15303779563559042No Hit
GAGGTGCACAATCGACCGATCCTGAAGATCGGAAGAGCACACGTCTGAAC525790.14251309299570877Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGCGTGCTTGTGGACTGCTTGGTGGGGCAGATCGGAAGAGCACACGTCTG506000.13714909955653137Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTGGAGACCGGGGTTCGACTCCCCGTATCGGAAGATCGGAAGAGCACACG505490.13701086627436965No Hit
CAATATAGATGGATACGAATAAGGCGTCCAGATCGGAAGAGCACACGTCT502920.1363142789505351Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATTCAATGAAGCGGAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC486000.13172818653058152Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCCAGGGGGCATGCCTGTTTGAGCGTCATTAGATCGGAAGAGCACACGTC466290.12638587674350793No Hit
GTGATAGTTTAATGGTCAGAATGGGCAGATCGGAAGAGCACACGTCTGAA464730.12596304552748383Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CCGATGTAGTGTAACGGCTATCACATCACAGATCGGAAGAGCACACGTCT449600.12186212482335278Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCGGTGCCCGAGTTGTAATTTGGAGAGGGCAGATCGGAAGAGCACACGTC448700.12161818373718503No Hit
GGTAAAATCCAACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGG437560.11859873518173096TruSeq Adapter, Index 11 (100% over 35bp)
AATGGCTTAGTGAGGCCTCAGGATCTGCTAGATCGGAAGAGCACACGTCT405120.1098060142536403Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCAGGGGGCATGCCTGTTTGAGCGTCATTTAGATCGGAAGAGCACACGT386400.10473203966135122No Hit
TGCTCTGCTAGGCGGACTACTTGCGTGCCAGATCGGAAGAGCACACGTCT383840.10403816279402965Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCGATGTAGTGTAACGGCTATCACATCACGTAGATCGGAAGAGCACACGT373920.10134938993315852No Hit
AGTTTCTTTACTTATTCAATGAAGCGGAGCAGATCGGAAGAGCACACGTC369990.10028418052355936No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)