FastQCFastQC Report
Thu 14 Sept 2023
SRR15164962_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR15164962_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences107910575
Total Bases7 Gbp
Sequences flagged as poor quality0
Sequence length65
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTCGTATGC1887877217.494830325943493RNA PCR Primer, Index 1 (100% over 50bp)
GGCATTAACGCGAACTCGGCCTACAATAGTGATGGAATTCTCGGGTGCCA17439921.6161455909210012No Hit
AGTGTACTCCGAAGAGGACTGGAATTCTCGGGTGCCAAGGAACTCCAGTC14884871.3793708355274725RNA PCR Primer, Index 1 (100% over 31bp)
TGGGATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTCGTATGC9653380.8945721955424665RNA PCR Primer, Index 1 (98% over 50bp)
CGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTCGTATGC7245640.6714485582159117RNA PCR Primer, Index 1 (98% over 50bp)
TGGTATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTCGTATGC6291100.5829919820184444RNA PCR Primer, Index 1 (98% over 50bp)
AGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTCGTATGC5697650.5279973718979812RNA PCR Primer, Index 1 (98% over 50bp)
CGTGTACTCCGAAGAGGACTGGAATTCTCGGGTGCCAAGGAACTCCAGTC5196740.48157838098814687RNA PCR Primer, Index 1 (100% over 31bp)
TGGAGTTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTCGTATGC3496060.32397751564200267RNA PCR Primer, Index 1 (98% over 50bp)
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATTGGAATTCTCGGGTGC3128560.2899215392003981No Hit
TCGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTCGTATGC3125720.2896583583212303RNA PCR Primer, Index 1 (98% over 50bp)
TGGATTTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTCGTATGC2733870.2533458838487331RNA PCR Primer, Index 1 (98% over 50bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCTCGATCTCGTATGC2667520.24719727422451415RNA PCR Primer, Index 1 (98% over 50bp)
TAGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTCGTATGC2301060.21323767387950626RNA PCR Primer, Index 1 (98% over 50bp)
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGATGGAATTCTCGGGTGCCA2293670.21255284757772816No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAA2288200.21204594637735924No Hit
GGCATTAACGCGAACTCGGCCTACTGGAATTCTCGGGTGCCAAGGAACTC2222120.20592235747052592RNA PCR Primer, Index 1 (100% over 26bp)
AGTTTACTCCGAAGAGGACTGGAATTCTCGGGTGCCAAGGAACTCCAGTC1962610.18187374128995235RNA PCR Primer, Index 1 (100% over 31bp)
CCCGGGGAGCCCGGCGGGTGCCGGCGCGGTGGAATTCTCGGGTGCCAAGG1936950.17949584644507732Illumina Small RNA Adapter 2 (100% over 21bp)
GGCATTAACGCGAACTCGGCCTATGGAATTCTCGGGTGCCAAGGAACTCC1683960.1560514342547058RNA PCR Primer, Index 1 (100% over 27bp)
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGATTGGAATTCTCGGGTGCC1609440.14914571625626125No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGGAATTCTCGGGTG1577000.14613952339703498No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCA1549860.14362447795315705No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAA1444640.13387381171863832No Hit
GGCATTAACGCGAACTCGGCCTACAATTGGAATTCTCGGGTGCCAAGGAA1425080.13206119974803213RNA PCR Primer, Index 1 (100% over 23bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACTATCTCGTATGC1416930.1313059447602795RNA PCR Primer, Index 1 (98% over 50bp)
GGATCCAAAAGCAGTGTACTCCGAAGAGGACTGGAATTCTCGGGTGCCAA1407290.1304126124802875No Hit
AGTGTACTCCGAAGAGGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA1362530.12626473355368553RNA PCR Primer, Index 1 (100% over 32bp)
GGCATTAACGCGAACTCGGCCTACAATAGTGACTGGAATTCTCGGGTGCC1349620.12506837258535597No Hit
CGTTTACTCCGAAGAGGACTGGAATTCTCGGGTGCCAAGGAACTCCAGTC1293350.11985386974353532RNA PCR Primer, Index 1 (100% over 31bp)
AGTGTACTCCGAAGAGGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGT1289210.11947021874362175RNA PCR Primer, Index 1 (100% over 30bp)
TGGATAACTGTGGTAATTCTAGAGCTAATACATGTGGAATTCTCGGGTGC1203530.11153031109323623No Hit
GGCATTAACGCGAACTCGGCCTACATGGAATTCTCGGGTGCCAAGGAACT1201750.11136535969713812RNA PCR Primer, Index 1 (100% over 25bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGCACGATCTCGTATGC1151040.10666609829481494RNA PCR Primer, Index 1 (98% over 50bp)
GGCATTAACGCGAACTCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAG1142250.10585153493992597RNA PCR Primer, Index 1 (100% over 29bp)
GCGAACTCGGCCTACAATAGTGATGGAATTCTCGGGTGCCAAGGAACTCC1123870.10414827277122748RNA PCR Primer, Index 1 (100% over 27bp)
TGCAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTCGTATGC1080560.10013476436391891RNA PCR Primer, Index 1 (98% over 50bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)