SRR15164963_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR15164963_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	101991247
Total Bases	6.6 Gbp
Sequences flagged as poor quality	0
Sequence length	65
%GC	52

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTCGTATGC	25318738	24.824422433034865	RNA PCR Primer, Index 2 (100% over 50bp)
AGTGTACTCCGAAGAGGACTGGAATTCTCGGGTGCCAAGGAACTCCAGTC	1422643	1.3948677380128511	RNA PCR Primer, Index 1 (100% over 31bp)
TGGGATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTCGTATGC	1355934	1.3294611448372624	RNA PCR Primer, Index 2 (98% over 50bp)
CGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTCGTATGC	937675	0.9193681100888982	RNA PCR Primer, Index 2 (98% over 50bp)
TGGTATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTCGTATGC	879473	0.8623024287564599	RNA PCR Primer, Index 2 (98% over 50bp)
AGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTCGTATGC	737478	0.7230796972214684	RNA PCR Primer, Index 2 (98% over 50bp)
GGCATTAACGCGAACTCGGCCTACAATAGTGATGGAATTCTCGGGTGCCA	513010	0.5029941441935698	No Hit
CGTGTACTCCGAAGAGGACTGGAATTCTCGGGTGCCAAGGAACTCCAGTC	482634	0.47321119625098806	RNA PCR Primer, Index 1 (100% over 31bp)
TCGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTCGTATGC	411223	0.4031944035354328	RNA PCR Primer, Index 2 (98% over 50bp)
TGGAGTTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTCGTATGC	407494	0.39953820743068275	RNA PCR Primer, Index 2 (98% over 50bp)
TGGATTTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTCGTATGC	313260	0.3071440042300885	RNA PCR Primer, Index 2 (98% over 50bp)
TAGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTCGTATGC	301460	0.2955743839468891	RNA PCR Primer, Index 2 (98% over 50bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGGATCTCGTATGC	204951	0.20094959717474578	RNA PCR Primer, Index 2 (98% over 50bp)
AGTTTACTCCGAAGAGGACTGGAATTCTCGGGTGCCAAGGAACTCCAGTC	189580	0.18587869604143578	RNA PCR Primer, Index 1 (100% over 31bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTATGTATCTCGTATGC	186840	0.1831921909926251	RNA PCR Primer, Index 2 (98% over 50bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGATGTATCTCGTATGC	181980	0.17842707619801923	RNA PCR Primer, Index 2 (98% over 50bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGCTGTATCTCGTATGC	181651	0.1781044994969029	RNA PCR Primer, Index 2 (98% over 50bp)
CGGTTGGCCCCGGATAGCCGGGTTGGAATTCTCGGGTGCCAAGGAACTCC	151944	0.1489774901958008	RNA PCR Primer, Index 1 (100% over 27bp)
TGCAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTCGTATGC	150061	0.14713125333196483	RNA PCR Primer, Index 2 (98% over 50bp)
GGCCCGAGGTGGGATCCCGAGGCCTTGGAATTCTCGGGTGCCAAGGAACT	138984	0.13627051741018523	RNA PCR Primer, Index 1 (100% over 25bp)
GCCCGAGGTGGGATCCCGAGGCCTGGAATTCTCGGGTGCCAAGGAACTCC	137835	0.13514395014701605	RNA PCR Primer, Index 1 (100% over 27bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTCTCTCGTATGC	131886	0.1293110966669522	RNA PCR Primer, Index 2 (98% over 50bp)
GGCATTAACGCGAACTCGGCCTACTGGAATTCTCGGGTGCCAAGGAACTC	128889	0.1263726092102786	RNA PCR Primer, Index 1 (100% over 26bp)
CGTTTACTCCGAAGAGGACTGGAATTCTCGGGTGCCAAGGAACTCCAGTC	122102	0.11971811659484857	RNA PCR Primer, Index 1 (100% over 31bp)
AGTGTACTCCGAAGAGGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGT	119577	0.11724241394950295	RNA PCR Primer, Index 1 (100% over 30bp)
GGCATTAACGCGAACTCGGCCTATGGAATTCTCGGGTGCCAAGGAACTCC	118926	0.1166041238813366	RNA PCR Primer, Index 1 (100% over 27bp)
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGATGGAATTCTCGGGTGCCA	118539	0.11622467955509946	No Hit

Adapter Content

Adapter graph

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content