FastQCFastQC Report
Thu 14 Sept 2023
SRR15164964_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR15164964_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58585854
Total Bases3.8 Gbp
Sequences flagged as poor quality0
Sequence length65
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCATCTCGTATGC56569969.655907721341743RNA PCR Primer, Index 3 (100% over 50bp)
GGCATTAACGCGAACTCGGCCTACAATAGTGATGGAATTCTCGGGTGCCA3875120.6614429483267411No Hit
AGTGTACTCCGAAGAGGACTGGAATTCTCGGGTGCCAAGGAACTCCAGTC3186650.5439282322316237RNA PCR Primer, Index 1 (100% over 31bp)
TGGGATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCATCTCGTATGC2607430.44506136242376876RNA PCR Primer, Index 3 (98% over 50bp)
CGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCATCTCGTATGC2145790.36626418384205855RNA PCR Primer, Index 3 (98% over 50bp)
TGGTATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCATCTCGTATGC1705310.29107879864651287RNA PCR Primer, Index 3 (98% over 50bp)
AGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCATCTCGTATGC1687170.2879824880593189RNA PCR Primer, Index 3 (98% over 50bp)
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGATGGAATTCTCGGGTGCCA1574570.26876283138246987No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGTATCTCGTATGC1464470.24996989887695414RNA PCR Primer, Index 3 (98% over 50bp)
CGTGTACTCCGAAGAGGACTGGAATTCTCGGGTGCCAAGGAACTCCAGTC1093400.18663208357430447RNA PCR Primer, Index 1 (100% over 31bp)
TGGAGTTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCATCTCGTATGC1011130.17258944454407033RNA PCR Primer, Index 3 (98% over 50bp)
TCGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCATCTCGTATGC985450.1682061338561353RNA PCR Primer, Index 3 (98% over 50bp)
CTCGCCCGTCACGTTGAACGCACGTTCTGGAATTCTCGGGTGCCAAGGAA974280.1662995302586184RNA PCR Primer, Index 1 (100% over 23bp)
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCTGGAATTCTCGGGTGC959940.16385184041185094No Hit
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGATTGGAATTCTCGGGTGCC956420.16325101277861376No Hit
TGGATAACTGTGGTAATTCTAGAGCTAATACATGTGGAATTCTCGGGTGC883120.15073946007512326No Hit
GGCATTAACGCGAACTCGGCCTACTGGAATTCTCGGGTGCCAAGGAACTC869110.1483480978189718RNA PCR Primer, Index 1 (100% over 26bp)
GACCGGGGTCCGGTGCGGAGAGCCGTTTGGAATTCTCGGGTGCCAAGGAA849670.14502989066268454RNA PCR Primer, Index 1 (100% over 23bp)
GGCATTAACGCGAACTCGGCCTATGGAATTCTCGGGTGCCAAGGAACTCC798290.13625985549344388RNA PCR Primer, Index 1 (100% over 27bp)
GGCCCGAGGTGGGATCCCGAGGCCTTGGAATTCTCGGGTGCCAAGGAACT792330.13524254506898542RNA PCR Primer, Index 1 (100% over 25bp)
GCCCGAGGTGGGATCCCGAGGCCTGGAATTCTCGGGTGCCAAGGAACTCC786960.13432594154896163RNA PCR Primer, Index 1 (100% over 27bp)
TGGATTTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCATCTCGTATGC783170.13367902770522044RNA PCR Primer, Index 3 (98% over 50bp)
GGATCCAAAAGCAGTGTACTCCGAAGAGGACTGGAATTCTCGGGTGCCAA760420.129795837746088No Hit
CGTTTTTTCACTGACCCGGTGAGGCGGGGTGGAATTCTCGGGTGCCAAGG760320.12977876877923467Illumina Small RNA Adapter 2 (100% over 21bp)
TAGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCATCTCGTATGC722200.12327207861474547RNA PCR Primer, Index 3 (98% over 50bp)
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATTGGAATTCTCGGGTGC700130.11950495763021565No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGAGGTATCTCGTATGC698710.11926257830089837RNA PCR Primer, Index 3 (97% over 38bp)
GGCATTAACGCGAACTCGGCCTACATGGAATTCTCGGGTGCCAAGGAACT689800.1177417333542667RNA PCR Primer, Index 1 (100% over 25bp)
GACGAAGCCGAGCGCACGGGGTCGGCGGCGATGGAATTCTCGGGTGCCAA665330.113564957165257No Hit
AGTGTACTCCGAAGAGGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGT664790.11347278474424902RNA PCR Primer, Index 1 (100% over 30bp)
GGCATTAACGCGAACTCGGCCTACAATTGGAATTCTCGGGTGCCAAGGAA636590.1086593360916101RNA PCR Primer, Index 1 (100% over 23bp)
CGAAGCCGAGCGCACGGGGTCGGCGGCGATGGAATTCTCGGGTGCCAAGG635750.10851595677004214Illumina Small RNA Adapter 2 (100% over 21bp)
CCGAGCGCACGGGGTCGGCGGCGATGGAATTCTCGGGTGCCAAGGAACTC635170.10841695676229283RNA PCR Primer, Index 1 (100% over 26bp)
CCCGGGGAGCCCGGCGGGTGCCGGCGCGGTGGAATTCTCGGGTGCCAAGG633840.10818993950314354Illumina Small RNA Adapter 2 (100% over 21bp)
TGGATAACTGTGGTAATTCTAGAGCTAATACATGGAATTCTCGGGTGCCA592600.10115069757283048No Hit
CTAAACCATTCGTAGACGACCTGCTTTGGAATTCTCGGGTGCCAAGGAAC587450.100271645779884RNA PCR Primer, Index 1 (100% over 24bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)