FastQCFastQC Report
Thu 14 Sept 2023
SRR15164965_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR15164965_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences126729464
Total Bases8.2 Gbp
Sequences flagged as poor quality0
Sequence length65
%GC55

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTCGTATGC67844735.353508794134883RNA PCR Primer, Index 1 (100% over 50bp)
AGTGTACTCCGAAGAGGACTGGAATTCTCGGGTGCCAAGGAACTCCAGTC46913803.701885774566205RNA PCR Primer, Index 1 (100% over 31bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGGAATTCTCGGGTGCCAA21868271.7255868769396832No Hit
GGCATTAACGCGAACTCGGCCTACAATAGTGATGGAATTCTCGGGTGCCA14434711.1390176794245734No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGTGGAATTCTCGGGTGCCAAG12352490.9747133468504214No Hit
GGCATTAACGCGAACTCGGCCTACAATAGTGACTGGAATTCTCGGGTGCC10789890.8514113182077374No Hit
AGTGTACTCCGAAGAGGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGT10404580.8210071810924727RNA PCR Primer, Index 1 (100% over 30bp)
TCGGTTGGCCCCGGATAGCCGGGTTGGAATTCTCGGGTGCCAAGGAACTC10268030.8102322598002939RNA PCR Primer, Index 1 (100% over 26bp)
CGGTTGGCCCCGGATAGCCGGGTTGGAATTCTCGGGTGCCAAGGAACTCC9635280.7603030657495718RNA PCR Primer, Index 1 (100% over 27bp)
CGCTTCTGGCGCCAAGCGTCCGTGGAATTCTCGGGTGCCAAGGAACTCCA5485530.4328535627673767RNA PCR Primer, Index 1 (100% over 28bp)
CGTGTACTCCGAAGAGGACTGGAATTCTCGGGTGCCAAGGAACTCCAGTC5304720.4185861624097139RNA PCR Primer, Index 1 (100% over 31bp)
AACGCGAACTCGGCCTACAATAGTGACTGGAATTCTCGGGTGCCAAGGAA4771670.3765241207048741RNA PCR Primer, Index 1 (100% over 23bp)
CTCGGTTGGCCCCGGATAGCCGGGTTGGAATTCTCGGGTGCCAAGGAACT4295220.33892828584834855RNA PCR Primer, Index 1 (100% over 25bp)
CGCCGAGGGCGCACCACCGGCCCGTCTCGCTGGAATTCTCGGGTGCCAAG4256870.3359021545297469No Hit
GAGGGCGCACCACCGGCCCGTCTCGCTGGAATTCTCGGGTGCCAAGGAAC3330770.26282522586854784RNA PCR Primer, Index 1 (100% over 24bp)
GGCATTAACGCGAACTCGGCCTACAATTGGAATTCTCGGGTGCCAAGGAA3223710.2543773088158883RNA PCR Primer, Index 1 (100% over 23bp)
CGCCGAGGGCGCACCACCGGCCCGTCTCGTGGAATTCTCGGGTGCCAAGG3018270.23816639830497505Illumina Small RNA Adapter 2 (100% over 21bp)
CTAAACCATTCGTAGACGACCTGCTTTGGAATTCTCGGGTGCCAAGGAAC2850310.2249129689367265RNA PCR Primer, Index 1 (100% over 24bp)
GGCATTAACGCGAACTCGGCCTACAATAGTGTGGAATTCTCGGGTGCCAA2766770.2183209738818117No Hit
GGCATTAACGCGAACTCGGCCTACAATAGTTGGAATTCTCGGGTGCCAAG2415630.19061313160765836No Hit
TGGAATTCTCGGGTGCCAAGGTGGAATTCTCGGGTGCCAAGGAACTCCAG2134380.16842018680044288RNA PCR Primer, Index 1 (100% over 29bp)
GAGGGCGCACCACCGGCCCGTCTCGTGGAATTCTCGGGTGCCAAGGAACT2060200.16256677294871222RNA PCR Primer, Index 1 (100% over 25bp)
GCCGAGCGCACGGGGTCGGCGGCGATGGAATTCTCGGGTGCCAAGGAACT1996060.15750559790894406RNA PCR Primer, Index 1 (100% over 25bp)
CCGAGCGCACGGGGTCGGCGGCGATGGAATTCTCGGGTGCCAAGGAACTC1981230.15633538858808715RNA PCR Primer, Index 1 (100% over 26bp)
CCGGGGTCCGGTGCGGAGAGCCGTTGGAATTCTCGGGTGCCAAGGAACTC1960180.1546743699634049RNA PCR Primer, Index 1 (100% over 26bp)
GCCCGAGGTGGGATCCCGAGGCCTGGAATTCTCGGGTGCCAAGGAACTCC1946830.15362094484988906RNA PCR Primer, Index 1 (100% over 27bp)
TACCCGTCGCCGCGCTCTCCCTCTCCCCTGGAATTCTCGGGTGCCAAGGA1815870.1432871206651675RNA PCR Primer, Index 1 (100% over 22bp)
CCGGGGTCCGGTGCGGAGAGCCGTTTGGAATTCTCGGGTGCCAAGGAACT1802480.14223053922172352RNA PCR Primer, Index 1 (100% over 25bp)
AACGCGAACTCGGCCTACAATAGTGATGGAATTCTCGGGTGCCAAGGAAC1734680.1368805599935308RNA PCR Primer, Index 1 (100% over 24bp)
CGAAGCCGAGCGCACGGGGTCGGCGGCGATGGAATTCTCGGGTGCCAAGG1729270.1364536663707502Illumina Small RNA Adapter 2 (100% over 21bp)
CTTTCGCCGAATCCCGGGGCCGAGGAAGTGGAATTCTCGGGTGCCAAGGA1645850.12987114030561986RNA PCR Primer, Index 1 (100% over 22bp)
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGATGGAATTCTCGGGTGCCA1644120.12973462903622793No Hit
CGAGCGCACGGGGTCGGCGGCGATGGAATTCTCGGGTGCCAAGGAACTCC1596920.1260101597210259RNA PCR Primer, Index 1 (100% over 27bp)
CGTTTACTCCGAAGAGGACTGGAATTCTCGGGTGCCAAGGAACTCCAGTC1591950.12561798572745483RNA PCR Primer, Index 1 (100% over 31bp)
TGGGATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTCGTATGC1573140.12413372157874826RNA PCR Primer, Index 1 (98% over 50bp)
TCGGTTGGCCCCGGATAGCCGGGTCCCTGGAATTCTCGGGTGCCAAGGAA1530330.1207556594731593RNA PCR Primer, Index 1 (100% over 23bp)
GGCATTAACGCGAACTCGGCCTACTGGAATTCTCGGGTGCCAAGGAACTC1497750.11818482874669145RNA PCR Primer, Index 1 (100% over 26bp)
CGCCGCGACCGGCTCCGGGACGGCCGGGTGGAATTCTCGGGTGCCAAGGA1415600.11170251615677945RNA PCR Primer, Index 1 (100% over 22bp)
GGCCCGAGGTGGGATCCCGAGGCCTTGGAATTCTCGGGTGCCAAGGAACT1379360.10884288124188704RNA PCR Primer, Index 1 (100% over 25bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCTGGAATTCTCGGGTGCCAAGG1309870.10335954707423049Illumina Small RNA Adapter 2 (100% over 21bp)
CGGTTGGCCCCGGATAGCCGGGTCCCTGGAATTCTCGGGTGCCAAGGAAC1306780.1031157205872819RNA PCR Primer, Index 1 (100% over 24bp)
CCGAGCGCACGGGGTCGGCGGCGATTGGAATTCTCGGGTGCCAAGGAACT1302270.10275984438788441RNA PCR Primer, Index 1 (100% over 25bp)
ATTAACGCGAACTCGGCCTACAATAGTGACTGGAATTCTCGGGTGCCAAG1293680.10208202253581691No Hit
TTAACGCGAACTCGGCCTACAATAGTGACTGGAATTCTCGGGTGCCAAGG1275620.10065693957326292Illumina Small RNA Adapter 2 (100% over 21bp)
CGAGGGCGCACCACCGGCCCGTCTCGCTGGAATTCTCGGGTGCCAAGGAA1275210.10062458719149953RNA PCR Primer, Index 1 (100% over 23bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)