FastQCFastQC Report
Thu 14 Sept 2023
SRR15164966_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR15164966_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19815235
Total Bases1.2 Gbp
Sequences flagged as poor quality0
Sequence length65
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTGTACTCCGAAGAGGACTGGAATTCTCGGGTGCCAAGGAACTCCAGTC13135736.629106341660848RNA PCR Primer, Index 1 (100% over 31bp)
AGTGTACTCCGAAGAGGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGT11339495.72261191956593RNA PCR Primer, Index 1 (100% over 30bp)
GGCATTAACGCGAACTCGGCCTACAATAGTGACTGGAATTCTCGGGTGCC3373421.7024375436375094No Hit
AACGCGAACTCGGCCTACAATAGTGACTGGAATTCTCGGGTGCCAAGGAA3112401.5707106173608338RNA PCR Primer, Index 1 (100% over 23bp)
GGCATTAACGCGAACTCGGCCTACAATAGTGATGGAATTCTCGGGTGCCA2706611.3659237450375936No Hit
AGTGTACTCCGAAGAGGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA2373151.1976390893168816RNA PCR Primer, Index 1 (100% over 32bp)
GCCCGAGGTGGGATCCCGAGGCCTGGAATTCTCGGGTGCCAAGGAACTCC1344650.6785940212165034RNA PCR Primer, Index 1 (100% over 27bp)
AACGCGAACTCGGCCTACAATAGTGATGGAATTCTCGGGTGCCAAGGAAC1212760.6120341242483373RNA PCR Primer, Index 1 (100% over 24bp)
GGCATTAACGCGAACTCGGCCTACAATTGGAATTCTCGGGTGCCAAGGAA1120260.5653528711620125RNA PCR Primer, Index 1 (100% over 23bp)
GGCATTAACGCGAACTCGGCCTACTGGAATTCTCGGGTGCCAAGGAACTC792880.4001365615901098RNA PCR Primer, Index 1 (100% over 26bp)
GGCCGCCGGTGAAATACCACTACTGGAATTCTCGGGTGCCAAGGAACTCC766020.3865813350182322RNA PCR Primer, Index 1 (100% over 27bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTCGTATGC683600.34498707686282803RNA PCR Primer, Index 1 (100% over 50bp)
CGGTTGGCCCCGGATAGCCGGGTTGGAATTCTCGGGTGCCAAGGAACTCC598860.30222200241379926RNA PCR Primer, Index 1 (100% over 27bp)
AAAGCGAACTCGGCCTACAATAGTGACTGGAATTCTCGGGTGCCAAGGAA535430.2702112793514687RNA PCR Primer, Index 1 (100% over 23bp)
CCGAGCGCACGGGGTCGGCGGCGATGGAATTCTCGGGTGCCAAGGAACTC525050.26497288576188976RNA PCR Primer, Index 1 (100% over 26bp)
AAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGT499310.25198288084900333RNA PCR Primer, Index 1 (100% over 30bp)
AGTCTACTCCGAAGAGGACTGGAATTCTCGGGTGCCAAGGAACTCCAGTC484400.2444583675136833RNA PCR Primer, Index 1 (100% over 31bp)
AACGCGAACTCGGCCTACAATTGGAATTCTCGGGTGCCAAGGAACTCCAG469880.23713067243461913RNA PCR Primer, Index 1 (100% over 29bp)
GGCATTAACGCGAACTCGGCCTACAATAGTGTGGAATTCTCGGGTGCCAA466170.23525837568921085No Hit
GCCCGAGGTGGGATCCCGAGGCCTTGGAATTCTCGGGTGCCAAGGAACTC455110.229676811806673RNA PCR Primer, Index 1 (100% over 26bp)
ATTAACGCGAACTCGGCCTACAATAGTGACTGGAATTCTCGGGTGCCAAG441630.22287396541095778No Hit
AACTGTGGTAATTCTAGAGCTTGGAATTCTCGGGTGCCAAGGAACTCCAG425520.21474385744100435RNA PCR Primer, Index 1 (100% over 29bp)
GCGAACTCGGCCTACAATAGTGATGGAATTCTCGGGTGCCAAGGAACTCC423360.21365378709866423RNA PCR Primer, Index 1 (100% over 27bp)
TGGGGAAGGTGGCTCGGGGGGTGGAATTCTCGGGTGCCAAGGAACTCCAG422740.21334089653743696RNA PCR Primer, Index 1 (100% over 29bp)
GGCATTAACGCGAACTCGGCCTACAATAGTTGGAATTCTCGGGTGCCAAG419390.21165027818241872No Hit
AGTCTACTCCGAAGAGGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGT415030.20944995100991734RNA PCR Primer, Index 1 (100% over 30bp)
CGGTTGGCCCCGGATAGCCGGTGGAATTCTCGGGTGCCAAGGAACTCCAG413230.20854155905796726RNA PCR Primer, Index 1 (100% over 29bp)
AATGCGAACTCGGCCTACAATAGTGACTGGAATTCTCGGGTGCCAAGGAA404620.20419641755447263RNA PCR Primer, Index 1 (100% over 23bp)
TGGGGAAGGTGGCTCGGGGGTGGAATTCTCGGGTGCCAAGGAACTCCAGT389090.19635901365792535RNA PCR Primer, Index 1 (100% over 30bp)
GGCCTTAACGCGAACTCGGCCTACAATAGTGACTGGAATTCTCGGGTGCC381010.19228134311806042No Hit
GGGCCGCCGGTGAAATACCACTACTGGAATTCTCGGGTGCCAAGGAACTC370910.1871842549432293RNA PCR Primer, Index 1 (100% over 26bp)
CGAGCGCACGGGGTCGGCGGCGATGGAATTCTCGGGTGCCAAGGAACTCC367070.1852463521124024RNA PCR Primer, Index 1 (100% over 27bp)
GGCATTAACGCGAACTCGGCCTATGGAATTCTCGGGTGCCAAGGAACTCC361280.18232435800029623RNA PCR Primer, Index 1 (100% over 27bp)
GCCAGAGGTGGGATCCCGAGGCCTGGAATTCTCGGGTGCCAAGGAACTCC358760.1810526092675661RNA PCR Primer, Index 1 (100% over 27bp)
CTCGCCCGTCACGTTGAACGCACGTTCTGGAATTCTCGGGTGCCAAGGAA336540.1698390152829376RNA PCR Primer, Index 1 (100% over 23bp)
GGCATTAACGCGAACTCGGCCTTGGAATTCTCGGGTGCCAAGGAACTCCA333880.16849661384283357RNA PCR Primer, Index 1 (100% over 28bp)
GGCCCGAGGTGGGATCCCGAGGCTGGAATTCTCGGGTGCCAAGGAACTCC327110.16508005077911012RNA PCR Primer, Index 1 (100% over 27bp)
ACTGTGGTAATTCTAGAGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGT323690.16335410607040493RNA PCR Primer, Index 1 (100% over 30bp)
GCCGCCGGTGAAATACCACTACTGGAATTCTCGGGTGCCAAGGAACTCCA316750.15985175043344174RNA PCR Primer, Index 1 (100% over 28bp)
AGCGTACTCCGAAGAGGACTGGAATTCTCGGGTGCCAAGGAACTCCAGTC315510.15922596931098723RNA PCR Primer, Index 1 (100% over 31bp)
CCGAGCGCACGGGGTCGGCGGCGATTGGAATTCTCGGGTGCCAAGGAACT312220.15756563068770066RNA PCR Primer, Index 1 (100% over 25bp)
GGCCTTAACGCGAACTCGGCCTACAATAGTGATGGAATTCTCGGGTGCCA307000.15493129402704534No Hit
GAGCGCACGGGGTCGGCGGCGATGGAATTCTCGGGTGCCAAGGAACTCCA296900.14983420585221421RNA PCR Primer, Index 1 (100% over 28bp)
CCGTGGCGCAATGAAGGTGAAGGGCTGGAATTCTCGGGTGCCAAGGAACT295320.14903683958328023RNA PCR Primer, Index 1 (100% over 25bp)
AGAGTACTCCGAAGAGGACTGGAATTCTCGGGTGCCAAGGAACTCCAGTC293720.1482293800704357RNA PCR Primer, Index 1 (100% over 31bp)
AACGCGAACTCGGCCTACAATAGTGTGGAATTCTCGGGTGCCAAGGAACT291110.14691221174010807RNA PCR Primer, Index 1 (100% over 25bp)
CGCCGCGACCGGCTCCGGGACGGCCGGGTGGAATTCTCGGGTGCCAAGGA286290.1444797399576639RNA PCR Primer, Index 1 (100% over 22bp)
ACTGACCCGGTGAGGCGGGGTGGAATTCTCGGGTGCCAAGGAACTCCAGT286270.14446964671375334RNA PCR Primer, Index 1 (100% over 30bp)
AGCGTACTCCGAAGAGGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGT284490.14357134800571378RNA PCR Primer, Index 1 (100% over 30bp)
GTCCGGTGCGGAGAGCCGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC272780.13766175369608283RNA PCR Primer, Index 1 (100% over 31bp)
AAGGCGAACTCGGCCTACAATAGTGACTGGAATTCTCGGGTGCCAAGGAA265720.1340988385956563RNA PCR Primer, Index 1 (100% over 23bp)
GGCATTAACGCGAACTCGGCCTACATGGAATTCTCGGGTGCCAAGGAACT251140.1267408637848605RNA PCR Primer, Index 1 (100% over 25bp)
AGAGTACTCCGAAGAGGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGT247190.12474744811252554RNA PCR Primer, Index 1 (100% over 30bp)
CGAGCGCACGGGGTCGGCGGCGATTGGAATTCTCGGGTGCCAAGGAACTC238680.12045277282858366RNA PCR Primer, Index 1 (100% over 26bp)
CGTTTTTTCACTGACCCGGTGAGGCGGGGTGGAATTCTCGGGTGCCAAGG227640.11488130218995636Illumina Small RNA Adapter 2 (100% over 21bp)
GAGCGCACGGGGTCGGCGGCGATTGGAATTCTCGGGTGCCAAGGAACTCC223780.11293330611521891RNA PCR Primer, Index 1 (100% over 27bp)
ATTAACGCGAACTCGGCCTACAATAGTGATGGAATTCTCGGGTGCCAAGG222390.11223182566343524Illumina Small RNA Adapter 2 (100% over 21bp)
AACGCGAACTCGGCCTACAATAGTTGGAATTCTCGGGTGCCAAGGAACTC220170.11111147558936345RNA PCR Primer, Index 1 (100% over 26bp)
GCTGAGAAGACGGTCGAACTTGGAATTCTCGGGTGCCAAGGAACTCCAGT219340.11069260596707534RNA PCR Primer, Index 1 (100% over 30bp)
GCCCGAGGTGGGATCCCGAGGCTGGAATTCTCGGGTGCCAAGGAACTCCA212460.10722053006184383RNA PCR Primer, Index 1 (100% over 28bp)
CAACTTCTTAGAGGGACAAGTGGCGTGGAATTCTCGGGTGCCAAGGAACT211240.10660484218329987RNA PCR Primer, Index 1 (100% over 25bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATGGAATTCTCGGGTGC208510.10522711438950888No Hit
GCGAACTCGGCCTACAATAGTGACTGGAATTCTCGGGTGCCAAGGAACTC208400.10517160154800081RNA PCR Primer, Index 1 (100% over 26bp)
AGCGCACGGGGTCGGCGGCGATGGAATTCTCGGGTGCCAAGGAACTCCAG208380.10516150830409025RNA PCR Primer, Index 1 (100% over 29bp)
GGCATTAACGCGAACTCGGCCTACAATATGGAATTCTCGGGTGCCAAGGA207360.10464675286465186RNA PCR Primer, Index 1 (100% over 22bp)
AAAGCGAACTCGGCCTACAATAGTGATGGAATTCTCGGGTGCCAAGGAAC207280.10460637988900966RNA PCR Primer, Index 1 (100% over 24bp)
CCGCGCGCACGGGGTCGGCGGCGATGGAATTCTCGGGTGCCAAGGAACTC207080.10450544744990406RNA PCR Primer, Index 1 (100% over 26bp)
CCGGGGTCCGGTGCGGAGAGCCGTTGGAATTCTCGGGTGCCAAGGAACTC198890.10037226406853111RNA PCR Primer, Index 1 (100% over 26bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)