FastQCFastQC Report
Sat 16 Sept 2023
SRR15164967_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR15164967_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences118048257
Total Bases7.6 Gbp
Sequences flagged as poor quality0
Sequence length65
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCTCGTATGC45765563.8768518200145894RNA PCR Primer, Index 5 (100% over 50bp)
GGCATTAACGCGAACTCGGCCTACAATAGTGATGGAATTCTCGGGTGCCA39052043.3081420253413825No Hit
GGCATTAACGCGAACTCGGCCTACAATAGTGACTGGAATTCTCGGGTGCC21631931.8324650062389318No Hit
GCGAACTCGGCCTACAATAGTGATGGAATTCTCGGGTGCCAAGGAACTCC13722971.1624881509262777RNA PCR Primer, Index 1 (100% over 27bp)
AACGCGAACTCGGCCTACAATAGTGACTGGAATTCTCGGGTGCCAAGGAA11729870.9936504187435821RNA PCR Primer, Index 1 (100% over 23bp)
GGCATTAACGCGAACTCGGCCTACAATTGGAATTCTCGGGTGCCAAGGAA11262830.9540869375140373RNA PCR Primer, Index 1 (100% over 23bp)
AGTGTACTCCGAAGAGGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGT10669080.903789710338544RNA PCR Primer, Index 1 (100% over 30bp)
GGCATTAACGCGAACTCGGCCTACAATAGTGTGGAATTCTCGGGTGCCAA9115490.7721833622668398No Hit
GGCATTAACGCGAACTCGGCCTACTGGAATTCTCGGGTGCCAAGGAACTC9056490.7671854062190855RNA PCR Primer, Index 1 (100% over 26bp)
GGCATTAACGCGAACTCGGCCTACATGGAATTCTCGGGTGCCAAGGAACT8486000.7188585596820798RNA PCR Primer, Index 1 (100% over 25bp)
AACGCGAACTCGGCCTACAATAGTGATGGAATTCTCGGGTGCCAAGGAAC8396670.7112913153813021RNA PCR Primer, Index 1 (100% over 24bp)
GGCCGCCGGTGAAATACCACTACTGGAATTCTCGGGTGCCAAGGAACTCC8326780.7053708552426996RNA PCR Primer, Index 1 (100% over 27bp)
GGCATTAACGCGAACTCGGCCTACAATAGTTGGAATTCTCGGGTGCCAAG7775290.6586535199753097No Hit
CTAAACCATTCGTAGACGACCTGCTTTGGAATTCTCGGGTGCCAAGGAAC7772560.6584222586192018RNA PCR Primer, Index 1 (100% over 24bp)
CGCGAACTCGGCCTACAATAGTGATGGAATTCTCGGGTGCCAAGGAACTC7719700.6539444288448918RNA PCR Primer, Index 1 (100% over 26bp)
ACGCGAACTCGGCCTACAATAGTGATGGAATTCTCGGGTGCCAAGGAACT7517720.6368344769376816RNA PCR Primer, Index 1 (100% over 25bp)
GGCATTAACGCGAACTCGGCCTACAATATGGAATTCTCGGGTGCCAAGGA7313610.6195440903460353RNA PCR Primer, Index 1 (100% over 22bp)
GGCATTAACGCGAACTCGGCCTACAATGGAATTCTCGGGTGCCAAGGAAC7309130.6191645845308839RNA PCR Primer, Index 1 (100% over 24bp)
AGTGTACTCCGAAGAGGACTGGAATTCTCGGGTGCCAAGGAACTCCAGTC6963870.5899172234283815RNA PCR Primer, Index 1 (100% over 31bp)
TTAACGCGAACTCGGCCTACAATAGTGATGGAATTCTCGGGTGCCAAGGA6689560.5666801162511023RNA PCR Primer, Index 1 (100% over 22bp)
GGCATTAACGCGAACTCGGCCTATGGAATTCTCGGGTGCCAAGGAACTCC6667260.56479105828729RNA PCR Primer, Index 1 (100% over 27bp)
GGCATTAACGCGAACTCGGCCTACAATAGTGGAATTCTCGGGTGCCAAGG6564420.556079366762696Illumina Small RNA Adapter 2 (100% over 21bp)
ACGCGAACTCGGCCTACAATAGTGACTGGAATTCTCGGGTGCCAAGGAAC6309200.5344593948557834RNA PCR Primer, Index 1 (100% over 24bp)
AGCGTGTACTCCGAAGAGGATCCAAAAGCTGGAATTCTCGGGTGCCAAGG6283610.5322916373089693Illumina Small RNA Adapter 2 (100% over 21bp)
ATTAACGCGAACTCGGCCTACAATAGTGATGGAATTCTCGGGTGCCAAGG5922990.5017431134116618Illumina Small RNA Adapter 2 (100% over 21bp)
AAAAGCAGTGTACTCCGAAGAGGACTGGAATTCTCGGGTGCCAAGGAACT5527790.4682652790036536RNA PCR Primer, Index 1 (100% over 25bp)
CGCGAACTCGGCCTACAATAGTGACTGGAATTCTCGGGTGCCAAGGAACT5510840.4668294255289173RNA PCR Primer, Index 1 (100% over 25bp)
ATTAACGCGAACTCGGCCTACAATAGTGACTGGAATTCTCGGGTGCCAAG5427080.45973402216349535No Hit
AAAAGCAGTGTACTCCGAAGAGGATGGAATTCTCGGGTGCCAAGGAACTC5348320.4530621743953407RNA PCR Primer, Index 1 (100% over 26bp)
TTAACGCGAACTCGGCCTACAATAGTGACTGGAATTCTCGGGTGCCAAGG5091670.43132106558760963Illumina Small RNA Adapter 2 (100% over 21bp)
GCCCGAGGTGGGATCCCGAGGCCTGGAATTCTCGGGTGCCAAGGAACTCC5021740.425397217004229RNA PCR Primer, Index 1 (100% over 27bp)
AGCGTGTACTCCGAAGAGGATCCAAATGGAATTCTCGGGTGCCAAGGAAC4909880.4159214311821648RNA PCR Primer, Index 1 (100% over 24bp)
GGATCCAAAAGCAGTGTACTCCGAAGAGGACTGGAATTCTCGGGTGCCAA4685760.3969359750902548No Hit
CGAACTCGGCCTACAATAGTGATGGAATTCTCGGGTGCCAAGGAACTCCA4662250.39494441667190394RNA PCR Primer, Index 1 (100% over 28bp)
GAACTCGGCCTACAATAGTGATGGAATTCTCGGGTGCCAAGGAACTCCAG4639840.39304604048495184RNA PCR Primer, Index 1 (100% over 29bp)
AGCGTGTACTCCGAAGAGGATCCAAAATGGAATTCTCGGGTGCCAAGGAA4416240.3741046341751577RNA PCR Primer, Index 1 (100% over 23bp)
AGCGTGTACTCCGAAGAGGATCCTGGAATTCTCGGGTGCCAAGGAACTCC4215560.3571048067232369RNA PCR Primer, Index 1 (100% over 27bp)
TAACGCGAACTCGGCCTACAATAGTGACTGGAATTCTCGGGTGCCAAGGA4144010.3510437261263417RNA PCR Primer, Index 1 (100% over 22bp)
GAACTCGGCCTACAATAGTGACTGGAATTCTCGGGTGCCAAGGAACTCCA4086680.3461872376480747RNA PCR Primer, Index 1 (100% over 28bp)
AGCGTGTACTCCGAAGAGGATCCAAAAGCATGGAATTCTCGGGTGCCAAG4035850.34188137144625524No Hit
GCATTAACGCGAACTCGGCCTACAATAGTGATGGAATTCTCGGGTGCCAA3989940.3379922839521468No Hit
TAACGCGAACTCGGCCTACAATAGTGATGGAATTCTCGGGTGCCAAGGAA3828630.3243275332731088RNA PCR Primer, Index 1 (100% over 23bp)
CAAAAGCAGTGTACTCCGAAGAGGATGGAATTCTCGGGTGCCAAGGAACT3631810.3076546907422784RNA PCR Primer, Index 1 (100% over 25bp)
GCGAACTCGGCCTACAATAGTGACTGGAATTCTCGGGTGCCAAGGAACTC3588130.3039545090445512RNA PCR Primer, Index 1 (100% over 26bp)
AACGCGAACTCGGCCTACAATAGTGTGGAATTCTCGGGTGCCAAGGAACT3517570.29797729245591487RNA PCR Primer, Index 1 (100% over 25bp)
AGGATCCAAAAGCAGTGTACTCCGAAGAGGATGGAATTCTCGGGTGCCAA3498510.2963626985191319No Hit
AGCGTGTACTCCGAAGAGGATCCAATGGAATTCTCGGGTGCCAAGGAACT3423440.2900034347817605RNA PCR Primer, Index 1 (100% over 25bp)
AGCGTGTACTCCGAAGAGGATCCAAAAGCAGTTGGAATTCTCGGGTGCCA3379920.2863168068631458No Hit
ATCCAAAAGCAGTGTACTCCGAAGAGGACTGGAATTCTCGGGTGCCAAGG3285780.27834210207779686Illumina Small RNA Adapter 2 (100% over 21bp)
GATCCAAAAGCAGTGTACTCCGAAGAGGATGGAATTCTCGGGTGCCAAGG3079750.26088907013680007Illumina Small RNA Adapter 2 (100% over 21bp)
AAGCAGTGTACTCCGAAGAGGATGGAATTCTCGGGTGCCAAGGAACTCCA3043610.257827610279752RNA PCR Primer, Index 1 (100% over 28bp)
AAAGCAGTGTACTCCGAAGAGGATGGAATTCTCGGGTGCCAAGGAACTCC3001730.25427990859704097RNA PCR Primer, Index 1 (100% over 27bp)
GGCATTAACGCGAACTCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAG2962510.25095753849207614RNA PCR Primer, Index 1 (100% over 29bp)
AGCAGTGTACTCCGAAGAGGATGGAATTCTCGGGTGCCAAGGAACTCCAG2942600.24927094010375772RNA PCR Primer, Index 1 (100% over 29bp)
AGCGTGTACTCCGAAGAGGATCCAAAAGTGGAATTCTCGGGTGCCAAGGA2940630.24910405919843442RNA PCR Primer, Index 1 (100% over 22bp)
GCCGCCGGTGAAATACCACTACTGGAATTCTCGGGTGCCAAGGAACTCCA2935240.24864746626458026RNA PCR Primer, Index 1 (100% over 28bp)
CATTAACGCGAACTCGGCCTACAATAGTGATGGAATTCTCGGGTGCCAAG2916830.247087934555442No Hit
AGGCATTAACGCGAACTCGGCCTACAATAGTGATGGAATTCTCGGGTGCC2803860.23751811939078438No Hit
AGGATCCAAAAGCAGTGTACTCCGAAGAGGACTGGAATTCTCGGGTGCCA2799650.23716148557788533No Hit
GCCCGAGGTGGGATCCCGAGGCCTTGGAATTCTCGGGTGCCAAGGAACTC2786980.23608819569440995RNA PCR Primer, Index 1 (100% over 26bp)
CGAACTCGGCCTACAATAGTGACTGGAATTCTCGGGTGCCAAGGAACTCC2785780.2359865423510658RNA PCR Primer, Index 1 (100% over 27bp)
AGCGTGTACTCCGAAGAGGATCCAAAAGCAGTGGAATTCTCGGGTGCCAA2771290.23475907823018513No Hit
ATCCAAAAGCAGTGTACTCCGAAGAGGATGGAATTCTCGGGTGCCAAGGA2740220.23212710374876608RNA PCR Primer, Index 1 (100% over 22bp)
AAAGCAGTGTACTCCGAAGAGGACTGGAATTCTCGGGTGCCAAGGAACTC2710780.22963320839205614RNA PCR Primer, Index 1 (100% over 26bp)
CATTAACGCGAACTCGGCCTACAATAGTGACTGGAATTCTCGGGTGCCAA2692720.22810332557472662No Hit
CAAAAGCAGTGTACTCCGAAGAGGACTGGAATTCTCGGGTGCCAAGGAAC2678330.2268843325657913RNA PCR Primer, Index 1 (100% over 24bp)
TTAACGCGAACTCGGCCTACAATAGTGTGGAATTCTCGGGTGCCAAGGAA2661640.225470503982113RNA PCR Primer, Index 1 (100% over 23bp)
CGGTTGGCCCCGGATAGCCGGGTTGGAATTCTCGGGTGCCAAGGAACTCC2654040.22482669947426667RNA PCR Primer, Index 1 (100% over 27bp)
AGCAGTGTACTCCGAAGAGGACTGGAATTCTCGGGTGCCAAGGAACTCCA2621210.22204563342260952RNA PCR Primer, Index 1 (100% over 28bp)
GATCCAAAAGCAGTGTACTCCGAAGAGGACTGGAATTCTCGGGTGCCAAG2612480.22130610534978082No Hit
ACGCGAACTCGGCCTACAATAGTGTGGAATTCTCGGGTGCCAAGGAACTC2594000.21974064386228084RNA PCR Primer, Index 1 (100% over 26bp)
AGCGTGTACTCCGAAGAGGATCTGGAATTCTCGGGTGCCAAGGAACTCCA2593630.21970930074808304RNA PCR Primer, Index 1 (100% over 28bp)
AAGCAGTGTACTCCGAAGAGGACTGGAATTCTCGGGTGCCAAGGAACTCC2575420.2181667112628355RNA PCR Primer, Index 1 (100% over 27bp)
CCAAAAGCAGTGTACTCCGAAGAGGATGGAATTCTCGGGTGCCAAGGAAC2408970.2040665454298067RNA PCR Primer, Index 1 (100% over 24bp)
GGATCCAAAAGCAGTGTACTCCGAAGAGGATGGAATTCTCGGGTGCCAAG2300740.1948982609713585No Hit
GAGGATCCAAAAGCAGTGTACTCCGAAGAGGATGGAATTCTCGGGTGCCA2255350.19105322325936586No Hit
CTAGACCATTCGTAGACGACCTGCTTTGGAATTCTCGGGTGCCAAGGAAC2242900.18999856982217028RNA PCR Primer, Index 1 (100% over 24bp)
GGCATTAACGCGAACTCGGCCTTGGAATTCTCGGGTGCCAAGGAACTCCA2186250.18519968490513164RNA PCR Primer, Index 1 (100% over 28bp)
AACTCGGCCTACAATAGTGACTGGAATTCTCGGGTGCCAAGGAACTCCAG2177110.18442542527332698RNA PCR Primer, Index 1 (100% over 29bp)
GGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGG2146100.18179853345907512Illumina Small RNA Adapter 2 (100% over 21bp)
GCGAACTCGGCCTACAATAGTGTGGAATTCTCGGGTGCCAAGGAACTCCA2143880.18161047477388845RNA PCR Primer, Index 1 (100% over 28bp)
CGCGAACTCGGCCTACAATAGTGTGGAATTCTCGGGTGCCAAGGAACTCC2117930.1794122212240711RNA PCR Primer, Index 1 (100% over 27bp)
TCCAAAAGCAGTGTACTCCGAAGAGGACTGGAATTCTCGGGTGCCAAGGA2097360.17766971349691338RNA PCR Primer, Index 1 (100% over 22bp)
TAACGCGAACTCGGCCTACAATAGTGTGGAATTCTCGGGTGCCAAGGAAC2081440.17632111247521426RNA PCR Primer, Index 1 (100% over 24bp)
GCATTAACGCGAACTCGGCCTACAATAGTGACTGGAATTCTCGGGTGCCA2055520.17412540025898054No Hit
GCAGTGTACTCCGAAGAGGACTGGAATTCTCGGGTGCCAAGGAACTCCAG1984700.16812615877928633RNA PCR Primer, Index 1 (100% over 29bp)
TCCAAAAGCAGTGTACTCCGAAGAGGATGGAATTCTCGGGTGCCAAGGAA1981280.16783644675075549RNA PCR Primer, Index 1 (100% over 23bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAA1938040.1641735379455878No Hit
CCAAAAGCAGTGTACTCCGAAGAGGACTGGAATTCTCGGGTGCCAAGGAA1891760.16025310733728154RNA PCR Primer, Index 1 (100% over 23bp)
AGGCATTAACGCGAACTCGGCCTACAATAGTGACTGGAATTCTCGGGTGC1789500.15159054826197052No Hit
ACTCGGCCTACAATAGTGACTGGAATTCTCGGGTGCCAAGGAACTCCAGT1778690.1506748210606786RNA PCR Primer, Index 1 (100% over 30bp)
GCATTAACGCGAACTCGGCCTACAATAGTGTGGAATTCTCGGGTGCCAAG1765560.14956256406225463No Hit
GGCATTAACGCGAACTCGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGT1757520.1488814866618488RNA PCR Primer, Index 1 (100% over 30bp)
AACGCGAACTCGGCCTACAATAGTTGGAATTCTCGGGTGCCAAGGAACTC1756250.14877390354014292RNA PCR Primer, Index 1 (100% over 26bp)
TTAACGCGAACTCGGCCTACAATAGTTGGAATTCTCGGGTGCCAAGGAAC1737220.1471618509369435RNA PCR Primer, Index 1 (100% over 24bp)
ATTAACGCGAACTCGGCCTACAATAGTGTGGAATTCTCGGGTGCCAAGGA1699630.1439775599566879RNA PCR Primer, Index 1 (100% over 22bp)
AACTCGGCCTACAATAGTGATGGAATTCTCGGGTGCCAAGGAACTCCAGT1688880.14306691542256317RNA PCR Primer, Index 1 (100% over 30bp)
GCAGTGTACTCCGAAGAGGATGGAATTCTCGGGTGCCAAGGAACTCCAGT1439410.12193403245250795RNA PCR Primer, Index 1 (100% over 30bp)
GCCCGAGGTGGGATCCCGAGGCTGGAATTCTCGGGTGCCAAGGAACTCCA1368670.11594156786237005RNA PCR Primer, Index 1 (100% over 28bp)
TTAACGCGAACTCGGCCTACAATTGGAATTCTCGGGTGCCAAGGAACTCC1314050.11131464651782194RNA PCR Primer, Index 1 (100% over 27bp)
CCGTGGCGCAATGAAGGTGAAGGGCTGGAATTCTCGGGTGCCAAGGAACT1312490.11118249717147455RNA PCR Primer, Index 1 (100% over 25bp)
TTAACGCGAACTCGGCCTACAATATGGAATTCTCGGGTGCCAAGGAACTC1278900.10833705066903275RNA PCR Primer, Index 1 (100% over 26bp)
CATTAACGCGAACTCGGCCTACAATAGTGTGGAATTCTCGGGTGCCAAGG1275690.10806512797558715Illumina Small RNA Adapter 2 (100% over 21bp)
GGATCCAAAAGCAGTGTACTCCGAAGAGGACCTGGAATTCTCGGGTGCCA1246470.10558986906515698No Hit
AGCGTGTACTCCGAAGAGGATCCATGGAATTCTCGGGTGCCAAGGAACTC1196830.10138480909548711RNA PCR Primer, Index 1 (100% over 26bp)
GAGGATCCAAAAGCAGTGTACTCCGAAGAGGACTGGAATTCTCGGGTGCC1185860.10045552811508263No Hit
GCATTAACGCGAACTCGGCCTACAATTGGAATTCTCGGGTGCCAAGGAAC1181870.10011753074846333RNA PCR Primer, Index 1 (100% over 24bp)

[FAIL]Adapter Content

Adapter graph