FastQCFastQC Report
Thu 14 Sept 2023
SRR15164971_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR15164971_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences97861414
Total Bases6.3 Gbp
Sequences flagged as poor quality0
Sequence length65
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CACGAATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTC4954008350.622692821503676RNA PCR Primer, Index 1 (100% over 44bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTCGTATGC1081191511.048190045567909RNA PCR Primer, Index 1 (100% over 50bp)
ACGAATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTCG61332746.2673057227642355RNA PCR Primer, Index 1 (100% over 45bp)
CACGAATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCTCGATCTC8300010.8481391858899565RNA PCR Primer, Index 1 (97% over 44bp)
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGTGGAATTCTCGGGT7069190.7223674491357748No Hit
CAGAAGCAGGTCGTCTACGAATGGAATTCTCGGGTGCCAAGGAACTCCAG6780450.6928624595593929RNA PCR Primer, Index 1 (100% over 29bp)
CACGGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTC6571960.6715578419907156RNA PCR Primer, Index 1 (100% over 44bp)
CATCACGAATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGAT6216450.6352299385332814RNA PCR Primer, Index 1 (100% over 41bp)
CAGGTCGTCTACGAATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAT4700890.4803619534865907RNA PCR Primer, Index 1 (100% over 35bp)
CACGAATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGCTCGATCTC3886960.39719025519087636RNA PCR Primer, Index 14 (97% over 38bp)
CACGAATGGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCT3713440.37945905829645993RNA PCR Primer, Index 1 (97% over 43bp)
AGTTTTATCCGGTAAAGCGAATGATTAGAGGTTGGAATTCTCGGGTGCCA3577790.3655976195071124No Hit
CACGTATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTC3434640.35096979081050267RNA PCR Primer, Index 1 (100% over 44bp)
CACGAATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGCACGATCTC3212930.3283142832986247RNA PCR Primer, Index 1 (97% over 44bp)
TGGAGTTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTCGTATGC1838050.18782172920575213RNA PCR Primer, Index 1 (98% over 50bp)
CCAGAAGCAGGTCGTCTACGAATGGAATTCTCGGGTGCCAAGGAACTCCA1754380.1792718834003359RNA PCR Primer, Index 1 (100% over 28bp)
CCCATCACGAATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACG1741850.17799150132860334RNA PCR Primer, Index 1 (100% over 39bp)
CGCCGCGACCGGCTCCGGGACGGCCGGGTGGAATTCTCGGGTGCCAAGGA1587680.16223759039492316RNA PCR Primer, Index 1 (100% over 22bp)
CACGAATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTCACGATCTC1540870.15745429552039786RNA PCR Primer, Index 1 (97% over 44bp)
CACAAATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTC1200370.12266019373069759RNA PCR Primer, Index 1 (100% over 44bp)
CACGAATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGCTGGATCTC1179590.12053678276097667RNA PCR Primer, Index 11 (97% over 37bp)
AACGAATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTC1137280.11621332183080861RNA PCR Primer, Index 1 (100% over 44bp)
CGTTTACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCCCTGGAATTCTCG1067750.10910837646388391No Hit
GGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGG1062880.10861073395076837Illumina Small RNA Adapter 2 (100% over 21bp)
AGAAGCAGGTCGTCTACGAATGGAATTCTCGGGTGCCAAGGAACTCCAGT1025320.10477265329519969RNA PCR Primer, Index 1 (100% over 30bp)
CACGAATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATTTC997610.10194109805116856RNA PCR Primer, Index 1 (97% over 44bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)