FastQCFastQC Report
Thu 14 Sept 2023
SRR15164972_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR15164972_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences67094406
Total Bases4.3 Gbp
Sequences flagged as poor quality0
Sequence length65
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CACGAATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTC2692595040.13143808144005RNA PCR Primer, Index 2 (100% over 44bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTCGTATGC55409658.258460474335221RNA PCR Primer, Index 2 (100% over 50bp)
ACGAATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTCG23738843.5381250711124856RNA PCR Primer, Index 2 (100% over 45bp)
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGTGGAATTCTCGGGT8599731.2817357679565715No Hit
CACGAATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGGATCTC8482251.2642261114883409RNA PCR Primer, Index 2 (97% over 44bp)
CACGGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTC5210420.7765803903234496RNA PCR Primer, Index 2 (100% over 44bp)
CTAAACCATTCGTAGACGACCTGCTTTGGAATTCTCGGGTGCCAAGGAAC4576640.6821194601529076RNA PCR Primer, Index 1 (100% over 24bp)
CGCCGCGACCGGCTCCGGGACGGCCGGGTGGAATTCTCGGGTGCCAAGGA4011740.5979246615582229RNA PCR Primer, Index 1 (100% over 22bp)
CATCACGAATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTAT3607580.5376871508483136RNA PCR Primer, Index 2 (100% over 41bp)
CAGAAGCAGGTCGTCTACGAATGGAATTCTCGGGTGCCAAGGAACTCCAG2888960.430581351297752RNA PCR Primer, Index 1 (100% over 29bp)
CACGTATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTC2660680.39655765042468666RNA PCR Primer, Index 2 (100% over 44bp)
CACGAATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGCTGTATCTC2557680.3812061470519614RNA PCR Primer, Index 2 (97% over 44bp)
CGCCGCGACCGGCTCCGGGACGGCCGGTGGAATTCTCGGGTGCCAAGGAA2181300.325109070941026RNA PCR Primer, Index 1 (100% over 23bp)
CACGAATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGCTGGATCTC1675770.24976299812535788RNA PCR Primer, Index 2 (97% over 38bp)
CACGAATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGATGGATCTC1495340.22287103935311686RNA PCR Primer, Index 2 (97% over 38bp)
CACGAATGGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCT1484960.22132396551807912RNA PCR Primer, Index 2 (97% over 43bp)
GTCCGGTGCGGAGAGCCGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC1418570.21142895281016424RNA PCR Primer, Index 1 (100% over 31bp)
CAGGTCGTCTACGAATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCG1416510.21112192274270972RNA PCR Primer, Index 2 (100% over 35bp)
CGGTTGGCCCCGGATAGCCGGGTTGGAATTCTCGGGTGCCAAGGAACTCC1365060.20345362324245037RNA PCR Primer, Index 1 (100% over 27bp)
CCGTGGCGCAATGAAGGTGAAGGGCTGGAATTCTCGGGTGCCAAGGAACT1340280.19976031980967235RNA PCR Primer, Index 1 (100% over 25bp)
CACGAATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTATGTATCTC1330550.19831012439397705RNA PCR Primer, Index 2 (97% over 44bp)
ACGAGACTCTGGCATGCTAACTAGTTACGCGACCCCTGGAATTCTCGGGT1085660.16181080729740718No Hit
CACGAATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGCTGGATCTC982740.14647122742244711RNA PCR Primer, Index 11 (97% over 37bp)
CACGAATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGATGTATCTC941750.1403619252549907RNA PCR Primer, Index 2 (97% over 44bp)
AGCCCGTGGACGGTGTGAGGCCGGTAGCGTGGAATTCTCGGGTGCCAAGG936170.13953026128586637Illumina Small RNA Adapter 2 (100% over 21bp)
CACGAATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGAGGTATCTC920550.13720219834720648RNA PCR Primer, Index 2 (97% over 44bp)
TGGAGTTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTCGTATGC873050.130122621549105RNA PCR Primer, Index 2 (98% over 50bp)
CACGAATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGATGTATCTC845340.1259926200106757RNA PCR Primer, Index 2 (97% over 44bp)
AACGAATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTC821810.12248562123047935RNA PCR Primer, Index 2 (100% over 44bp)
AGTTTTATCCGGTAAAGCGAATGATTAGAGGTTGGAATTCTCGGGTGCCA820880.12234701056895861No Hit
GGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGG813960.12131562801226678Illumina Small RNA Adapter 2 (100% over 21bp)
CACGAATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGAGGTATCTC789760.11770876993828666RNA PCR Primer, Index 3 (97% over 38bp)
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGGTGGAATTCTCGGG787460.11736596937753649No Hit
GGTTGGCCCCGGATAGCCGGGTTGGAATTCTCGGGTGCCAAGGAACTCCA747390.11139378743438015RNA PCR Primer, Index 1 (100% over 28bp)
AAACCATTCGTAGACGACCTGCTTTGGAATTCTCGGGTGCCAAGGAACTC731700.10905529143517569RNA PCR Primer, Index 1 (100% over 26bp)
CGTTTACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCCCTGGAATTCTCG729780.10876912748881032No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCTGGAATTCTCGGGTG692820.10326047152127704No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)