FastQCFastQC Report
Thu 14 Sept 2023
SRR15164973_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR15164973_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55091852
Total Bases3.5 Gbp
Sequences flagged as poor quality0
Sequence length65
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CACGAATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCATCTC31184335.660425066124116RNA PCR Primer, Index 3 (100% over 44bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCATCTCGTATGC18535623.3644939001143035RNA PCR Primer, Index 3 (100% over 50bp)
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGTGGAATTCTCGGGT12161762.2075424148021017No Hit
CTAAACCATTCGTAGACGACCTGCTTTGGAATTCTCGGGTGCCAAGGAAC7396901.3426486370434598RNA PCR Primer, Index 1 (100% over 24bp)
CGCCGCGACCGGCTCCGGGACGGCCGGGTGGAATTCTCGGGTGCCAAGGA6589341.1960643472286974RNA PCR Primer, Index 1 (100% over 22bp)
ACGTACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTTGGAATTCTC5514731.001006464622028No Hit
GGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGG4480500.813278159536187Illumina Small RNA Adapter 2 (100% over 21bp)
CCGTGGCGCAATGAAGGTGAAGGGCTGGAATTCTCGGGTGCCAAGGAACT3950790.7171278250003286RNA PCR Primer, Index 1 (100% over 25bp)
CGCCGCGACCGGCTCCGGGACGGCCGGTGGAATTCTCGGGTGCCAAGGAA3665130.6652762372192534RNA PCR Primer, Index 1 (100% over 23bp)
CGTTTACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCCCTGGAATTCTCG3628460.6586200805157176No Hit
GTCCGGTGCGGAGAGCCGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC3612980.6558102276176884RNA PCR Primer, Index 1 (100% over 31bp)
ACGAATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCATCTCG2974750.5399618803884103RNA PCR Primer, Index 3 (100% over 45bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAA2499350.45366962795151633No Hit
AGTTTTATCCGGTAAAGCGAATGATTAGAGGTTGGAATTCTCGGGTGCCA2485240.4511084506652635No Hit
ACTGTGGTAATTCTAGAGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGT2421420.43952416048747095RNA PCR Primer, Index 1 (100% over 30bp)
AACTGTGGTAATTCTAGAGCTTGGAATTCTCGGGTGCCAAGGAACTCCAG2224400.40376206630338RNA PCR Primer, Index 1 (100% over 29bp)
CTGTGGTAATTCTAGAGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC2033480.3691072138943523RNA PCR Primer, Index 1 (100% over 31bp)
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCTGGAATTCTCGGGTG1884780.3421159266891227No Hit
GCGGTCGGCGTCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGG1841040.33417645861678424RNA PCR Primer, Index 3 (100% over 38bp)
GTCCGGTGCGGAGAGCCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA1775070.32220191109204316RNA PCR Primer, Index 1 (100% over 32bp)
TCCGGTGCGGAGAGCCGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA1749340.31753152898181747RNA PCR Primer, Index 1 (100% over 32bp)
CGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCTGGAATTCTCGGGTGCCAA1647900.29911864280765144No Hit
CGGTTGGCCCCGGATAGCCGGGTTGGAATTCTCGGGTGCCAAGGAACTCC1580280.2868445954585081RNA PCR Primer, Index 1 (100% over 27bp)
CTCTAAGTCAGAATCCCGCCCAGGCGGAACGATACTGGAATTCTCGGGTG1477620.2682102609293294No Hit
CGTACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTTGGAATTCTCG1448420.2629100216126334No Hit
GTCCCCGCTCGGCGGGGTCCCCGCGTCGTCTGGAATTCTCGGGTGCCAAG1427510.25911454201975276No Hit
GGTCCGGTGCGGAGAGCCGTTGGAATTCTCGGGTGCCAAGGAACTCCAGT1327920.24103745867900755RNA PCR Primer, Index 1 (100% over 30bp)
CGAACGAGACTCTGGCATGCTAACTAGTTACGCGACCCCTGGAATTCTCG1303060.23652499465801222No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGTGGAATTCTCGGGTGCCAAGG1294570.23498393192517833Illumina Small RNA Adapter 2 (100% over 21bp)
GGTTGGCCCCGGATAGCCGGGTTGGAATTCTCGGGTGCCAAGGAACTCCA1286750.2335644842725563RNA PCR Primer, Index 1 (100% over 28bp)
ATCCGGTAAAGCGAATGATTAGAGGTTGGAATTCTCGGGTGCCAAGGAAC1229070.2230946964716307RNA PCR Primer, Index 1 (100% over 24bp)
GTCCGGTGCGGAGAGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC1190960.216177158103162RNA PCR Primer, Index 1 (100% over 33bp)
AAACCATTCGTAGACGACCTGCTTTGGAATTCTCGGGTGCCAAGGAACTC1159330.21043583722689155RNA PCR Primer, Index 1 (100% over 26bp)
CCGAGCGCACGGGGTCGGCGGCGATTGGAATTCTCGGGTGCCAAGGAACT1136750.20633722750870673RNA PCR Primer, Index 1 (100% over 25bp)
TAAACCATTCGTAGACGACCTGCTTTGGAATTCTCGGGTGCCAAGGAACT1109690.20142543038850824RNA PCR Primer, Index 1 (100% over 25bp)
GGTTGGCCCCGGATAGCCGGGTGGAATTCTCGGGTGCCAAGGAACTCCAG1086430.19720339043966065RNA PCR Primer, Index 1 (100% over 29bp)
GGCCGCCGGTGAAATACCACTACTGGAATTCTCGGGTGCCAAGGAACTCC1009670.18327029557837338RNA PCR Primer, Index 1 (100% over 27bp)
ACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTTGGAATTCTCGGGT1004250.18228648403397293No Hit
ACGAGACTCTGGCATGCTAACTAGTTACGCGACCCCTGGAATTCTCGGGT1000720.18164573592479702No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGGTGGAATTCTCGGG997440.18105036657689416No Hit
TGCGCCGCGACCGGCTCCGGGACGGCCGGGTGGAATTCTCGGGTGCCAAG993320.18030252459111376No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCTGGAATTCTCGGGTGCCAA976860.17731478694889402No Hit
CCGAGCGCACGGGGTCGGCGGCGATGGAATTCTCGGGTGCCAAGGAACTC956710.17365725879028354RNA PCR Primer, Index 1 (100% over 26bp)
CTAAACCATTCGTAGACGACCTTGGAATTCTCGGGTGCCAAGGAACTCCA905410.16434553697704699RNA PCR Primer, Index 1 (100% over 28bp)
GGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAG901620.16365759495614707No Hit
ATTCGTAGACGACCTGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC885120.1606625967121236RNA PCR Primer, Index 1 (100% over 31bp)
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAG863460.15673098083542372No Hit
ACGTACGCAGTTTTATCCGGTAAAGCGAATGATTAGATGGAATTCTCGGG859460.1560049206550544No Hit
CGCCGCGACCGGCTCCGGGACGGCCGGGATGGAATTCTCGGGTGCCAAGG845400.15345281912105624Illumina Small RNA Adapter 2 (100% over 21bp)
CGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGTCTGGAATTCTCGGG842640.15295183759660141No Hit
CCGAGCGCACGGGGTCGGCGGCGTGGAATTCTCGGGTGCCAAGGAACTCC839950.15246356212530301RNA PCR Primer, Index 1 (100% over 27bp)
GTCCGGTGCGGAGAGCCGTTTGGAATTCTCGGGTGCCAAGGAACTCCAGT828910.15045963602748372RNA PCR Primer, Index 1 (100% over 30bp)
CGTTTTTTCACTGACCCGGTGAGGCGGGGTGGAATTCTCGGGTGCCAAGG821350.1490873822865857Illumina Small RNA Adapter 2 (100% over 21bp)
CTCTAAGTCAGAATCCCGCCCAGGCGGAACGATATGGAATTCTCGGGTGC810660.14714698645454866No Hit
CGGGGAGGTAGTGACGAAAAATAACAATGGAATTCTCGGGTGCCAAGGAA804870.1460960143434641RNA PCR Primer, Index 1 (100% over 23bp)
AGGCCCGGTGGGGAAGGTGGCTCGGGGGTGGAATTCTCGGGTGCCAAGGA764280.13872831866316637RNA PCR Primer, Index 1 (100% over 22bp)
GGCCCGAGGTGGGATCCCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTC740880.13448086660800584RNA PCR Primer, Index 1 (100% over 31bp)
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACTGGAATTCTCGGGTGCC723670.13135699268196685No Hit
CGAGCGCACGGGGTCGGCGGCGATTGGAATTCTCGGGTGCCAAGGAACTC710590.12898277589215915RNA PCR Primer, Index 1 (100% over 26bp)
ACGTACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTGGAATTCTCG676470.12278948255360883No Hit
AAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGT669890.12159511355690131RNA PCR Primer, Index 1 (100% over 30bp)
GCCCGAGGTGGGATCCCGAGGCCTGGAATTCTCGGGTGCCAAGGAACTCC662680.1202863900817856RNA PCR Primer, Index 1 (100% over 27bp)
TTTACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCCCTGGAATTCTCGGG657090.11927172097971947No Hit
AAAGCGAATGATTAGAGGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC645220.11711713739447352RNA PCR Primer, Index 1 (100% over 31bp)
GGGGGGCCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAAGGA642260.11657985286100021RNA PCR Primer, Index 1 (100% over 22bp)
ATTAAATCAGTTATGGTTCCTTTGGTCTGGAATTCTCGGGTGCCAAGGAA641250.11639652266545696RNA PCR Primer, Index 1 (100% over 23bp)
GCTAACTAGTTACGCGACCCCTGGAATTCTCGGGTGCCAAGGAACTCCAG630650.11447246318747825RNA PCR Primer, Index 1 (100% over 29bp)
GGTCCGGTGCGGAGAGCCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTC628260.11403864222970757RNA PCR Primer, Index 1 (100% over 31bp)
CGAGCGCACGGGGTCGGCGGCGATGGAATTCTCGGGTGCCAAGGAACTCC622960.11307661249071824RNA PCR Primer, Index 1 (100% over 27bp)
CGCCGCGACCGGCTCCGGGACGGCTGGAATTCTCGGGTGCCAAGGAACTC603270.10950258125285026RNA PCR Primer, Index 1 (100% over 26bp)
CCGTGGCGCAATGAAGGTGAAGGGTGGAATTCTCGGGTGCCAAGGAACTC598920.1087129908066986RNA PCR Primer, Index 1 (100% over 26bp)
AACCATTCGTAGACGACCTGCTTTGGAATTCTCGGGTGCCAAGGAACTCC597280.10841530613274718RNA PCR Primer, Index 1 (100% over 27bp)
GCCCGAGGTGGGATCCCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA594870.10797785487407466RNA PCR Primer, Index 1 (100% over 32bp)
AAGGCCCGGTGGGGAAGGTGGCTCGGGGGTGGAATTCTCGGGTGCCAAGG592470.10754221876585308Illumina Small RNA Adapter 2 (100% over 21bp)
CGGTTGGCCCCGGATAGCCGGTGGAATTCTCGGGTGCCAAGGAACTCCAG588740.10686516764765869RNA PCR Primer, Index 1 (100% over 29bp)
AGCGCACGGGGTCGGCGGCGTGGAATTCTCGGGTGCCAAGGAACTCCAGT569230.10332380911790731RNA PCR Primer, Index 1 (100% over 30bp)
ACGGGGTCGGCGGCGATTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC568050.10310962136469835RNA PCR Primer, Index 1 (100% over 33bp)
CGAGCGCACGGGGTCGGCGGCGTGGAATTCTCGGGTGCCAAGGAACTCCA567410.10299345173583926RNA PCR Primer, Index 1 (100% over 28bp)
CACGAATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGTATCTC565040.10256326107897044RNA PCR Primer, Index 3 (97% over 44bp)
GCGCCGCGACCGGCTCCGGGACGGCCGGGTGGAATTCTCGGGTGCCAAGG562060.1020223462445953Illumina Small RNA Adapter 2 (100% over 21bp)
CGGTTGGCCCCGGATAGCCGGGTGGAATTCTCGGGTGCCAAGGAACTCCA557180.10113655282454473RNA PCR Primer, Index 1 (100% over 28bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)