FastQCFastQC Report
Thu 14 Sept 2023
SRR15164974_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR15164974_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48523739
Total Bases3.1 Gbp
Sequences flagged as poor quality0
Sequence length65
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTCGTATGC2150339344.315202091083705RNA PCR Primer, Index 1 (100% over 50bp)
AGTGTACTCCGAAGAGGACTGGAATTCTCGGGTGCCAAGGAACTCCAGTC5545851.1429148112432144RNA PCR Primer, Index 1 (100% over 31bp)
AGTGTACTCCGAAGAGGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGT5168071.0650601347929927RNA PCR Primer, Index 1 (100% over 30bp)
CTAAACCATTCGTAGACGACCTGCTTTGGAATTCTCGGGTGCCAAGGAAC3280820.6761267922902644RNA PCR Primer, Index 1 (100% over 24bp)
CCGTGGCGCAATGAAGGTGAAGGGCTGGAATTCTCGGGTGCCAAGGAACT3207890.6610970354118837RNA PCR Primer, Index 1 (100% over 25bp)
AACTGTGGTAATTCTAGAGCTTGGAATTCTCGGGTGCCAAGGAACTCCAG2909450.5995931187413237RNA PCR Primer, Index 1 (100% over 29bp)
CCCGGGGAGCCCGGCGGGTGCCGGCGCGGGTGGAATTCTCGGGTGCCAAG2252990.4643067592132585No Hit
CGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCTGGAATTCTCGGGTGCCAA1791190.3691368466061529No Hit
CGGTTGGCCCCGGATAGCCGGGTTGGAATTCTCGGGTGCCAAGGAACTCC1511630.31152380899584015RNA PCR Primer, Index 1 (100% over 27bp)
GGCATTAACGCGAACTCGGCCTATGGAATTCTCGGGTGCCAAGGAACTCC1461430.30117835725725917RNA PCR Primer, Index 1 (100% over 27bp)
GGCATTAACGCGAACTCGGCCTACTGGAATTCTCGGGTGCCAAGGAACTC1456190.3000984734502838RNA PCR Primer, Index 1 (100% over 26bp)
ACTGTGGTAATTCTAGAGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGT1392120.28689462697835383RNA PCR Primer, Index 1 (100% over 30bp)
CCCGGGGAGCCCGGCGGGTGCCGGCGCGGTGGAATTCTCGGGTGCCAAGG1256330.25891038611018824Illumina Small RNA Adapter 2 (100% over 21bp)
GCTAACTAGTTACGCGACCCCTGGAATTCTCGGGTGCCAAGGAACTCCAG1133080.23351044732970805RNA PCR Primer, Index 1 (100% over 29bp)
GGCATTAACGCGAACTCGGCCTACAATAGTGACTGGAATTCTCGGGTGCC1132540.23339916159387472No Hit
CCGAGCGCACGGGGTCGGCGGCGATGGAATTCTCGGGTGCCAAGGAACTC1122630.2313568622566369RNA PCR Primer, Index 1 (100% over 26bp)
CTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTCGTATG1099320.22655302799316435RNA PCR Primer, Index 1 (100% over 49bp)
GTCCCCGCTCGGCGGGGTCCCCGCGTCGTCTGGAATTCTCGGGTGCCAAG1087400.22409649841699134No Hit
CCGAGCGCACGGGGTCGGCGGCGATTGGAATTCTCGGGTGCCAAGGAACT1038330.2139839223848764RNA PCR Primer, Index 1 (100% over 25bp)
CGCCGCGACCGGCTCCGGGACGGCCGGGTGGAATTCTCGGGTGCCAAGGA908940.1873186235710319RNA PCR Primer, Index 1 (100% over 22bp)
GGCATTAACGCGAACTCGGCCTACATGGAATTCTCGGGTGCCAAGGAACT830220.1710956363028826RNA PCR Primer, Index 1 (100% over 25bp)
CTAGACCATTCGTAGACGACCTGCTTTGGAATTCTCGGGTGCCAAGGAAC730810.1506087566747484RNA PCR Primer, Index 1 (100% over 24bp)
GAGCGCACGGGGTCGGCGGCGATGGAATTCTCGGGTGCCAAGGAACTCCA720290.14844074567295812RNA PCR Primer, Index 1 (100% over 28bp)
GGCATTAACGCGAACTCGGCCTACAATTGGAATTCTCGGGTGCCAAGGAA702730.14482189841141468RNA PCR Primer, Index 1 (100% over 23bp)
AGTGTACTCCGAAGAGGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA699640.14418509670081278RNA PCR Primer, Index 1 (100% over 32bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATTTCGTATGC680810.14030452187536496RNA PCR Primer, Index 1 (98% over 50bp)
GAGCGCACGGGGTCGGCGGCGATTGGAATTCTCGGGTGCCAAGGAACTCC673480.13879392105377533RNA PCR Primer, Index 1 (100% over 27bp)
CTCTAAGTCAGAATCCCGCCCAGGCGGAACGATACTGGAATTCTCGGGTG673190.13873415649193893No Hit
AATAGTGACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGAT672920.13867851362402225RNA PCR Primer, Index 1 (100% over 41bp)
CGAGCGCACGGGGTCGGCGGCGATGGAATTCTCGGGTGCCAAGGAACTCC654790.1349421980857658RNA PCR Primer, Index 1 (100% over 27bp)
CGAGCGCACGGGGTCGGCGGCGATTGGAATTCTCGGGTGCCAAGGAACTC642950.13250215528527182RNA PCR Primer, Index 1 (100% over 26bp)
CTCGCCCGTCACGTTGAACGCACGTTCGTTGGAATTCTCGGGTGCCAAGG641990.13230431397712367Illumina Small RNA Adapter 2 (100% over 21bp)
AAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGT633900.13063708878658342RNA PCR Primer, Index 1 (100% over 30bp)
CTCGCCCGTCACGTTGAACGCACGTTCTGGAATTCTCGGGTGCCAAGGAA624190.12863600638854314RNA PCR Primer, Index 1 (100% over 23bp)
GGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTCGTATGCC598690.12338084664085759RNA PCR Primer, Index 1 (100% over 50bp)
AAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC594230.12246170889675258RNA PCR Primer, Index 1 (100% over 31bp)
CCGTGGCGCAATGAAGGTGAAGGGCCTGGAATTCTCGGGTGCCAAGGAAC585360.12063373764334197RNA PCR Primer, Index 1 (100% over 24bp)
GTTCTACAGTCCGACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAT580760.11968574804179868RNA PCR Primer, Index 1 (100% over 35bp)
GGCATTAACGCGAACTCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAG555560.11449241370290941RNA PCR Primer, Index 1 (100% over 29bp)
CCGTGGCGCAATGAAGGTGAAGGGTGGAATTCTCGGGTGCCAAGGAACTC542630.11182773858378886RNA PCR Primer, Index 1 (100% over 26bp)
TCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTCGTAT540620.11141350834485363RNA PCR Primer, Index 1 (100% over 48bp)
CGTTTTTTCACTGACCCGGTGAGGCGGGGTGGAATTCTCGGGTGCCAAGG531030.1094371561103319Illumina Small RNA Adapter 2 (100% over 21bp)
TAACTGTGGTAATTCTAGAGCTTGGAATTCTCGGGTGCCAAGGAACTCCA513750.10587601256366497RNA PCR Primer, Index 1 (100% over 28bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)