FastQCFastQC Report
Thu 14 Sept 2023
SRR15164975_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR15164975_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33282668
Total Bases2.1 Gbp
Sequences flagged as poor quality0
Sequence length65
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTCGTATGC770063523.137072424602497RNA PCR Primer, Index 2 (100% over 50bp)
CTAAACCATTCGTAGACGACCTGCTTTGGAATTCTCGGGTGCCAAGGAAC9462872.8431825237087365RNA PCR Primer, Index 1 (100% over 24bp)
AACTGTGGTAATTCTAGAGCTTGGAATTCTCGGGTGCCAAGGAACTCCAG5788061.7390613036190488RNA PCR Primer, Index 1 (100% over 29bp)
CCGTGGCGCAATGAAGGTGAAGGGCTGGAATTCTCGGGTGCCAAGGAACT5611161.6859105165487336RNA PCR Primer, Index 1 (100% over 25bp)
CGCCGCGACCGGCTCCGGGACGGCCGGGTGGAATTCTCGGGTGCCAAGGA3965041.1913227629467686RNA PCR Primer, Index 1 (100% over 22bp)
ACTGTGGTAATTCTAGAGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGT3961071.1901299499186784RNA PCR Primer, Index 1 (100% over 30bp)
CGGTTGGCCCCGGATAGCCGGGTTGGAATTCTCGGGTGCCAAGGAACTCC2917350.8765373016369961RNA PCR Primer, Index 1 (100% over 27bp)
CGCCGCGACCGGCTCCGGGACGGCCGGTGGAATTCTCGGGTGCCAAGGAA2303270.6920328622693349RNA PCR Primer, Index 1 (100% over 23bp)
CCCGGGGAGCCCGGCGGGTGCCGGCGCGGGTGGAATTCTCGGGTGCCAAG2060100.6189708108736955No Hit
CTAGACCATTCGTAGACGACCTGCTTTGGAATTCTCGGGTGCCAAGGAAC2043320.6139291477474101RNA PCR Primer, Index 1 (100% over 24bp)
CCGAGCGCACGGGGTCGGCGGCGATGGAATTCTCGGGTGCCAAGGAACTC1844770.5542734735087944RNA PCR Primer, Index 1 (100% over 26bp)
GGCCGCCGGTGAAATACCACTACTGGAATTCTCGGGTGCCAAGGAACTCC1832100.5504666873460986RNA PCR Primer, Index 1 (100% over 27bp)
GCTAACTAGTTACGCGACCCCTGGAATTCTCGGGTGCCAAGGAACTCCAG1797330.54001980850814RNA PCR Primer, Index 1 (100% over 29bp)
CGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCTGGAATTCTCGGGTGCCAA1506050.45250278613481343No Hit
CCGAGCGCACGGGGTCGGCGGCGATTGGAATTCTCGGGTGCCAAGGAACT1368420.4111509329720802RNA PCR Primer, Index 1 (100% over 25bp)
GGTTGGCCCCGGATAGCCGGGTTGGAATTCTCGGGTGCCAAGGAACTCCA1354240.40689045721935513RNA PCR Primer, Index 1 (100% over 28bp)
CGAGCGCACGGGGTCGGCGGCGATGGAATTCTCGGGTGCCAAGGAACTCC1216240.36542743508423065RNA PCR Primer, Index 1 (100% over 27bp)
GCCCGAGGTGGGATCCCGAGGCCTGGAATTCTCGGGTGCCAAGGAACTCC1077480.3237360658706808RNA PCR Primer, Index 1 (100% over 27bp)
GAGCGCACGGGGTCGGCGGCGATGGAATTCTCGGGTGCCAAGGAACTCCA1048730.3150979362591966RNA PCR Primer, Index 1 (100% over 28bp)
CCCGGGGAGCCCGGCGGGTGCCGGCGCGGTGGAATTCTCGGGTGCCAAGG1021490.3069134962377415Illumina Small RNA Adapter 2 (100% over 21bp)
CCGAGCGCACGGGGTCGGCGGCGTGGAATTCTCGGGTGCCAAGGAACTCC991090.2977796131007286RNA PCR Primer, Index 1 (100% over 27bp)
CGGTTGGCCCCGGATAGCCGGTGGAATTCTCGGGTGCCAAGGAACTCCAG990690.29765943042787313RNA PCR Primer, Index 1 (100% over 29bp)
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGTGGAATTCTCGGGT980220.29451364896588217No Hit
CGAGCGCACGGGGTCGGCGGCGATTGGAATTCTCGGGTGCCAAGGAACTC942220.283096295044616RNA PCR Primer, Index 1 (100% over 26bp)
GTCCCCGCTCGGCGGGGTCCCCGCGTCGTCTGGAATTCTCGGGTGCCAAG932910.2802990433339058No Hit
CTGTGGTAATTCTAGAGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC925640.2781147232547583RNA PCR Primer, Index 1 (100% over 31bp)
AGTGTACTCCGAAGAGGACTGGAATTCTCGGGTGCCAAGGAACTCCAGTC909040.2731271423312578RNA PCR Primer, Index 1 (100% over 31bp)
AAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGT863780.2595284728976655RNA PCR Primer, Index 1 (100% over 30bp)
GTCCGGTGCGGAGAGCCGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC860140.2584348105746811RNA PCR Primer, Index 1 (100% over 31bp)
CCGTGGCGCAATGAAGGTGAAGGGTGGAATTCTCGGGTGCCAAGGAACTC816410.24529583986476083RNA PCR Primer, Index 1 (100% over 26bp)
AGTGTACTCCGAAGAGGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGT808400.2428891818408308RNA PCR Primer, Index 1 (100% over 30bp)
GAGCGCACGGGGTCGGCGGCGATTGGAATTCTCGGGTGCCAAGGAACTCC784790.23579539957553883RNA PCR Primer, Index 1 (100% over 27bp)
AAACCATTCGTAGACGACCTGCTTTGGAATTCTCGGGTGCCAAGGAACTC754170.2265954159684554RNA PCR Primer, Index 1 (100% over 26bp)
TAAACCATTCGTAGACGACCTGCTTTGGAATTCTCGGGTGCCAAGGAACT750980.22563695915243331RNA PCR Primer, Index 1 (100% over 25bp)
TCACTGACCCGGTGAGGCGGGGTGGAATTCTCGGGTGCCAAGGAACTCCA730570.21950463826998484RNA PCR Primer, Index 1 (100% over 28bp)
GTCCGGTGCGGAGAGCCGTTCTGGAATTCTCGGGTGCCAAGGAACTCCAG727000.2184320079147501RNA PCR Primer, Index 1 (100% over 29bp)
GGTCCGGTGCGGAGAGCCGTTGGAATTCTCGGGTGCCAAGGAACTCCAGT703360.21132921194899398RNA PCR Primer, Index 1 (100% over 30bp)
GGGCCGCCGGTGAAATACCACTACTGGAATTCTCGGGTGCCAAGGAACTC680570.2044818041630557RNA PCR Primer, Index 1 (100% over 26bp)
TGCGCCGCGACCGGCTCCGGGACGGCCGGGTGGAATTCTCGGGTGCCAAG676850.20336410530550014No Hit
TAACTGTGGTAATTCTAGAGCTTGGAATTCTCGGGTGCCAAGGAACTCCA663120.19923883505973738RNA PCR Primer, Index 1 (100% over 28bp)
AACCATTCGTAGACGACCTGCTTTGGAATTCTCGGGTGCCAAGGAACTCC620080.18630717946049277RNA PCR Primer, Index 1 (100% over 27bp)
GCCGAGCGCACGGGGTCGGCGGCGATGGAATTCTCGGGTGCCAAGGAACT616660.18527961760757883RNA PCR Primer, Index 1 (100% over 25bp)
CGTGGCGCAATGAAGGTGAAGGGCTGGAATTCTCGGGTGCCAAGGAACTC616180.18513539840015228RNA PCR Primer, Index 1 (100% over 26bp)
AGGCCCGGTGGGGAAGGTGGCTCGGGGGTGGAATTCTCGGGTGCCAAGGA601950.18085989981332026RNA PCR Primer, Index 1 (100% over 22bp)
CGAGCGCACGGGGTCGGCGGCGTGGAATTCTCGGGTGCCAAGGAACTCCA595510.17892495878034778RNA PCR Primer, Index 1 (100% over 28bp)
GAGCGCACGGGGTCGGCGGCGTGGAATTCTCGGGTGCCAAGGAACTCCAG575440.17289479316982642RNA PCR Primer, Index 1 (100% over 29bp)
CGTTTTTTCACTGACCCGGTGAGGCGGGGTGGAATTCTCGGGTGCCAAGG565190.16981511217790593Illumina Small RNA Adapter 2 (100% over 21bp)
GCCGAGCGCACGGGGTCGGCGGCGATTGGAATTCTCGGGTGCCAAGGAAC554710.16666632614909357RNA PCR Primer, Index 1 (100% over 24bp)
AACTGTGGTAATTCTAGAGCTATGGAATTCTCGGGTGCCAAGGAACTCCA547380.16446397866901777RNA PCR Primer, Index 1 (100% over 28bp)
TCGGTTGGCCCCGGATAGCCGGGTTGGAATTCTCGGGTGCCAAGGAACTC544200.16350852641981706RNA PCR Primer, Index 1 (100% over 26bp)
CCGTGGCGCAATGAAGGTGAAGGGCCTGGAATTCTCGGGTGCCAAGGAAC534320.16054001440028784RNA PCR Primer, Index 1 (100% over 24bp)
CGCCGCGACCGGCTCCGGGACGGCCGGGATGGAATTCTCGGGTGCCAAGG511890.15380077101991946Illumina Small RNA Adapter 2 (100% over 21bp)
AGCGCACGGGGTCGGCGGCGATGGAATTCTCGGGTGCCAAGGAACTCCAG509950.15321788505657058RNA PCR Primer, Index 1 (100% over 29bp)
CGGTTGGCCCCGGATAGCCGGGTGGAATTCTCGGGTGCCAAGGAACTCCA506700.15224140083962018RNA PCR Primer, Index 1 (100% over 28bp)
CTAAACCATTCGTAGACGACTGGAATTCTCGGGTGCCAAGGAACTCCAGT481760.1447480111870839RNA PCR Primer, Index 1 (100% over 30bp)
ATTCGTAGACGACCTGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC476390.14313455880399972RNA PCR Primer, Index 1 (100% over 31bp)
CTCGCCCGTCACGTTGAACGCACGTTCTGGAATTCTCGGGTGCCAAGGAA469080.14093822045756668RNA PCR Primer, Index 1 (100% over 23bp)
AACTGTGGTAATTCTAGAGTGGAATTCTCGGGTGCCAAGGAACTCCAGTC463830.1393608228763391RNA PCR Primer, Index 1 (100% over 31bp)
GCCCGAGGTGGGATCCCGAGGCTGGAATTCTCGGGTGCCAAGGAACTCCA450940.13548793624357278RNA PCR Primer, Index 1 (100% over 28bp)
CTCGCCCGTCACGTTGAACGCACGTTCGTTGGAATTCTCGGGTGCCAAGG445660.13390152496188107Illumina Small RNA Adapter 2 (100% over 21bp)
TACCTGGTTGATCCTGCCAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG443120.13313836498924905RNA PCR Primer, Index 1 (100% over 29bp)
CTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTCGTATG431980.12979127755022524RNA PCR Primer, Index 2 (100% over 49bp)
CGGTTGGCCCCGGATAGCCGTGGAATTCTCGGGTGCCAAGGAACTCCAGT427480.1284392224806016RNA PCR Primer, Index 1 (100% over 30bp)
AAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC413040.12410062799052048RNA PCR Primer, Index 1 (100% over 31bp)
AGCGCACGGGGTCGGCGGCGATTGGAATTCTCGGGTGCCAAGGAACTCCA410850.12344262785663697RNA PCR Primer, Index 1 (100% over 28bp)
AAGGCCCGGTGGGGAAGGTGGCTCGGGGGTGGAATTCTCGGGTGCCAAGG401090.1205101706389644Illumina Small RNA Adapter 2 (100% over 21bp)
CTCTAAGTCAGAATCCCGCCCAGGCGGAACGATACTGGAATTCTCGGGTG387300.11636687299227334No Hit
GGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGG378110.11360567608341976Illumina Small RNA Adapter 2 (100% over 21bp)
GGTCCGGTGCGGAGAGCCGTTCTGGAATTCTCGGGTGCCAAGGAACTCCA374410.112493986359507RNA PCR Primer, Index 1 (100% over 28bp)
AGACCATTCGTAGACGACCTGCTTTGGAATTCTCGGGTGCCAAGGAACTC373220.11213644290776208RNA PCR Primer, Index 1 (100% over 26bp)
GTGCGCCGCGACCGGCTCCGGGACGGCCGGGTGGAATTCTCGGGTGCCAA367970.11055904532653452No Hit
GTCCGGTGCGGAGAGCCGTTTGGAATTCTCGGGTGCCAAGGAACTCCAGT367910.11054101792560621RNA PCR Primer, Index 1 (100% over 30bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGGATCTCGTATGC362710.10897864317848556RNA PCR Primer, Index 2 (98% over 50bp)
GCCGCCGGTGAAATACCACTACTGGAATTCTCGGGTGCCAAGGAACTCCA357910.10753645110422036RNA PCR Primer, Index 1 (100% over 28bp)
CATTCGTAGACGACCTGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGT340680.10235958247097258RNA PCR Primer, Index 1 (100% over 30bp)
GCCCGAGGTGGGATCCCGAGGCCTTGGAATTCTCGGGTGCCAAGGAACTC336500.10110367353963332RNA PCR Primer, Index 1 (100% over 26bp)

[FAIL]Adapter Content

Adapter graph