FastQCFastQC Report
Thu 14 Sept 2023
SRR15164976_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR15164976_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences59537488
Total Bases3.8 Gbp
Sequences flagged as poor quality0
Sequence length65
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAAACCATTCGTAGACGACCTGCTTTGGAATTCTCGGGTGCCAAGGAAC26799504.501281612687455RNA PCR Primer, Index 1 (100% over 24bp)
AACTGTGGTAATTCTAGAGCTTGGAATTCTCGGGTGCCAAGGAACTCCAG23824494.0015947599267205RNA PCR Primer, Index 1 (100% over 29bp)
CCGTGGCGCAATGAAGGTGAAGGGCTGGAATTCTCGGGTGCCAAGGAACT17704992.973754955869149RNA PCR Primer, Index 1 (100% over 25bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCATCTCGTATGC14345152.4094315164926003RNA PCR Primer, Index 3 (100% over 50bp)
ACTGTGGTAATTCTAGAGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGT10611191.7822703571235654RNA PCR Primer, Index 1 (100% over 30bp)
CGCCGCGACCGGCTCCGGGACGGCCGGGTGGAATTCTCGGGTGCCAAGGA7630951.2817050662265093RNA PCR Primer, Index 1 (100% over 22bp)
CTAGACCATTCGTAGACGACCTGCTTTGGAATTCTCGGGTGCCAAGGAAC5276750.8862903318997939RNA PCR Primer, Index 1 (100% over 24bp)
CGCCGCGACCGGCTCCGGGACGGCCGGTGGAATTCTCGGGTGCCAAGGAA5271890.8854740394824854RNA PCR Primer, Index 1 (100% over 23bp)
CGGTTGGCCCCGGATAGCCGGGTTGGAATTCTCGGGTGCCAAGGAACTCC4630070.7776730519769326RNA PCR Primer, Index 1 (100% over 27bp)
CGAGCGCACGGGGTCGGCGGCGATGGAATTCTCGGGTGCCAAGGAACTCC4271660.7174740056214667RNA PCR Primer, Index 1 (100% over 27bp)
CCGAGCGCACGGGGTCGGCGGCGATGGAATTCTCGGGTGCCAAGGAACTC4138580.6951217021450418RNA PCR Primer, Index 1 (100% over 26bp)
CCGAGCGCACGGGGTCGGCGGCGTGGAATTCTCGGGTGCCAAGGAACTCC3595540.6039119420019871RNA PCR Primer, Index 1 (100% over 27bp)
CGAGCGCACGGGGTCGGCGGCGTGGAATTCTCGGGTGCCAAGGAACTCCA3343790.5616276588625976RNA PCR Primer, Index 1 (100% over 28bp)
GAGCGCACGGGGTCGGCGGCGATGGAATTCTCGGGTGCCAAGGAACTCCA3306680.5553946112069761RNA PCR Primer, Index 1 (100% over 28bp)
CTGTGGTAATTCTAGAGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC3147000.5285745344177101RNA PCR Primer, Index 1 (100% over 31bp)
GCCCGAGGTGGGATCCCGAGGCCTGGAATTCTCGGGTGCCAAGGAACTCC2986430.501604971980007RNA PCR Primer, Index 1 (100% over 27bp)
GAGCGCACGGGGTCGGCGGCGTGGAATTCTCGGGTGCCAAGGAACTCCAG2786570.46803620602871254RNA PCR Primer, Index 1 (100% over 29bp)
TAAACCATTCGTAGACGACCTGCTTTGGAATTCTCGGGTGCCAAGGAACT2659870.4467554963017586RNA PCR Primer, Index 1 (100% over 25bp)
AGGCCCGGTGGGGAAGGTGGCTCGGGGGTGGAATTCTCGGGTGCCAAGGA2570080.43167424194987875RNA PCR Primer, Index 1 (100% over 22bp)
TCACTGACCCGGTGAGGCGGGGTGGAATTCTCGGGTGCCAAGGAACTCCA2547510.42788335309007325RNA PCR Primer, Index 1 (100% over 28bp)
GGCCGCCGGTGAAATACCACTACTGGAATTCTCGGGTGCCAAGGAACTCC2521730.42355330812747755RNA PCR Primer, Index 1 (100% over 27bp)
TAACTGTGGTAATTCTAGAGCTTGGAATTCTCGGGTGCCAAGGAACTCCA2451890.4118228837602285RNA PCR Primer, Index 1 (100% over 28bp)
AAACCATTCGTAGACGACCTGCTTTGGAATTCTCGGGTGCCAAGGAACTC2445660.4107764842211684RNA PCR Primer, Index 1 (100% over 26bp)
CGAGCGCACGGGGTCGGCGGCGATTGGAATTCTCGGGTGCCAAGGAACTC2395640.4023750548561941RNA PCR Primer, Index 1 (100% over 26bp)
GTCCGGTGCGGAGAGCCGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC2284440.3836977468716853RNA PCR Primer, Index 1 (100% over 31bp)
CCGAGCGCACGGGGTCGGCGGCGATTGGAATTCTCGGGTGCCAAGGAACT2259680.37953902253988275RNA PCR Primer, Index 1 (100% over 25bp)
AACCATTCGTAGACGACCTGCTTTGGAATTCTCGGGTGCCAAGGAACTCC2146290.3604938790833768RNA PCR Primer, Index 1 (100% over 27bp)
AGTGTACTCCGAAGAGGACTGGAATTCTCGGGTGCCAAGGAACTCCAGTC2112440.35480838560068234RNA PCR Primer, Index 1 (100% over 31bp)
AACTGTGGTAATTCTAGAGCTATGGAATTCTCGGGTGCCAAGGAACTCCA2106370.3537888598860604RNA PCR Primer, Index 1 (100% over 28bp)
CGTGGCGCAATGAAGGTGAAGGGCTGGAATTCTCGGGTGCCAAGGAACTC2060720.3461214218510529RNA PCR Primer, Index 1 (100% over 26bp)
AAGGCCCGGTGGGGAAGGTGGCTCGGGGGTGGAATTCTCGGGTGCCAAGG1933730.3247920033173049Illumina Small RNA Adapter 2 (100% over 21bp)
AAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGT1925590.3234247974990144RNA PCR Primer, Index 1 (100% over 30bp)
GAGCGCACGGGGTCGGCGGCGATTGGAATTCTCGGGTGCCAAGGAACTCC1832300.3077556782375501RNA PCR Primer, Index 1 (100% over 27bp)
GGTCCGGTGCGGAGAGCCGTTGGAATTCTCGGGTGCCAAGGAACTCCAGT1808860.30381866295736226RNA PCR Primer, Index 1 (100% over 30bp)
AGTGTACTCCGAAGAGGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGT1778030.2986404129109377RNA PCR Primer, Index 1 (100% over 30bp)
AGCGCACGGGGTCGGCGGCGATGGAATTCTCGGGTGCCAAGGAACTCCAG1704100.2862230264064886RNA PCR Primer, Index 1 (100% over 29bp)
AACTGTGGTAATTCTAGAGTGGAATTCTCGGGTGCCAAGGAACTCCAGTC1683170.2827075942471741RNA PCR Primer, Index 1 (100% over 31bp)
GCCGAGCGCACGGGGTCGGCGGCGATGGAATTCTCGGGTGCCAAGGAACT1606950.26990557613045413RNA PCR Primer, Index 1 (100% over 25bp)
GCTAACTAGTTACGCGACCCCTGGAATTCTCGGGTGCCAAGGAACTCCAG1598500.26848630227731474RNA PCR Primer, Index 1 (100% over 29bp)
CCGTGGCGCAATGAAGGTGAAGGGTGGAATTCTCGGGTGCCAAGGAACTC1570270.26374475187801005RNA PCR Primer, Index 1 (100% over 26bp)
GGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGG1544170.25936095926653807Illumina Small RNA Adapter 2 (100% over 21bp)
GCCCGAGGTGGGATCCCGAGGCTGGAATTCTCGGGTGCCAAGGAACTCCA1486050.24959904253938292RNA PCR Primer, Index 1 (100% over 28bp)
CGGTTGGCCCCGGATAGCCGGTGGAATTCTCGGGTGCCAAGGAACTCCAG1377340.23133995844769265RNA PCR Primer, Index 1 (100% over 29bp)
CGGTTGGCCCCGGATAGCCGGGTGGAATTCTCGGGTGCCAAGGAACTCCA1357590.2280227207436095RNA PCR Primer, Index 1 (100% over 28bp)
CGGGGAGGTAGTGACGAAAAATAACAATGGAATTCTCGGGTGCCAAGGAA1261410.21186819302823123RNA PCR Primer, Index 1 (100% over 23bp)
AGCGCACGGGGTCGGCGGCGTGGAATTCTCGGGTGCCAAGGAACTCCAGT1227840.20622972873830353RNA PCR Primer, Index 1 (100% over 30bp)
TTGGTGACTCTAGATAACCTCTGGAATTCTCGGGTGCCAAGGAACTCCAG1178350.19791731891678063RNA PCR Primer, Index 1 (100% over 29bp)
AGACCATTCGTAGACGACCTGCTTTGGAATTCTCGGGTGCCAAGGAACTC1177220.19772752253168624RNA PCR Primer, Index 1 (100% over 26bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAA1174980.19735128898955226No Hit
CTCGGTTGGCCCCGGATAGCCGGGTTGGAATTCTCGGGTGCCAAGGAACT1170650.19662401611569505RNA PCR Primer, Index 1 (100% over 25bp)
CGTTTTTTCACTGACCCGGTGAGGCGGGGTGGAATTCTCGGGTGCCAAGG1091800.18338025950977307Illumina Small RNA Adapter 2 (100% over 21bp)
TCGGTTGGCCCCGGATAGCCGGGTTGGAATTCTCGGGTGCCAAGGAACTC1050820.1764972012255539RNA PCR Primer, Index 1 (100% over 26bp)
ACCATTCGTAGACGACCTGCTTTGGAATTCTCGGGTGCCAAGGAACTCCA1019890.1713021550388555RNA PCR Primer, Index 1 (100% over 28bp)
AGCGCACGGGGTCGGCGGCGATTGGAATTCTCGGGTGCCAAGGAACTCCA1015070.17049258107765647RNA PCR Primer, Index 1 (100% over 28bp)
TTGGTGACTCTAGATAACCTCGGTGGAATTCTCGGGTGCCAAGGAACTCC972920.1634130079522334RNA PCR Primer, Index 1 (100% over 27bp)
TAGACCATTCGTAGACGACCTGCTTTGGAATTCTCGGGTGCCAAGGAACT952320.15995300305582258RNA PCR Primer, Index 1 (100% over 25bp)
GCCGAGCGCACGGGGTCGGCGGCGATTGGAATTCTCGGGTGCCAAGGAAC933970.15687091131557315RNA PCR Primer, Index 1 (100% over 24bp)
CGCCGCGACCGGCTCCGGGACGGCCGGGATGGAATTCTCGGGTGCCAAGG929470.1561150850032504Illumina Small RNA Adapter 2 (100% over 21bp)
CGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCTGGAATTCTCGGGTGCCAA926990.15569854072445918No Hit
CTAAACCATTCGTAGACGACTGGAATTCTCGGGTGCCAAGGAACTCCAGT903380.15173297200580582RNA PCR Primer, Index 1 (100% over 30bp)
GGTTGGCCCCGGATAGCCGGGTTGGAATTCTCGGGTGCCAAGGAACTCCA902520.1515885251994508RNA PCR Primer, Index 1 (100% over 28bp)
CGCGACCGGCTCCGGGACGGCCGGGTGGAATTCTCGGGTGCCAAGGAACT897110.15067985401063613RNA PCR Primer, Index 1 (100% over 25bp)
CGCGGCGTGGGAAATGTGGCGTATGGAATTCTCGGGTGCCAAGGAACTCC819810.13769643757895866RNA PCR Primer, Index 1 (100% over 27bp)
CATTCGTAGACGACCTGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGT806790.13550958011530484RNA PCR Primer, Index 1 (100% over 30bp)
GCTGAGAAGACGGTCGAACTTGTGGAATTCTCGGGTGCCAAGGAACTCCA805450.1352845118356354RNA PCR Primer, Index 1 (100% over 28bp)
CGGGGAGGTAGTGACGAAAAATAACATGGAATTCTCGGGTGCCAAGGAAC799290.13424986959476692RNA PCR Primer, Index 1 (100% over 24bp)
GCCGAGCGCACGGGGTCGGCGGCGTGGAATTCTCGGGTGCCAAGGAACTC778180.13070420438295952RNA PCR Primer, Index 1 (100% over 26bp)
TTGGTGACTCTAGATAACCTCGTGGAATTCTCGGGTGCCAAGGAACTCCA777790.13063869943589157RNA PCR Primer, Index 1 (100% over 28bp)
AACTGTGGTAATTCTAGAGCTGGAATTCTCGGGTGCCAAGGAACTCCAGT754180.12667313071723818RNA PCR Primer, Index 1 (100% over 30bp)
CGCGGCGTGGGAAATGTGGCGTACTGGAATTCTCGGGTGCCAAGGAACTC746230.12533783756546799RNA PCR Primer, Index 1 (100% over 26bp)
CGCGCGCTACACTGACTGGCTCAGCTGGAATTCTCGGGTGCCAAGGAACT738420.12402605901008117RNA PCR Primer, Index 1 (100% over 25bp)
CGCCGCGACCGGCTCCGGGACGGCTGGAATTCTCGGGTGCCAAGGAACTC730450.12268740663025622RNA PCR Primer, Index 1 (100% over 26bp)
GGGCCGCCGGTGAAATACCACTACTGGAATTCTCGGGTGCCAAGGAACTC719570.12085998656846254RNA PCR Primer, Index 1 (100% over 26bp)
ATTCGTAGACGACCTGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC718010.12059796678019066RNA PCR Primer, Index 1 (100% over 31bp)
TTGGTGACTCTAGATAACCTTGGAATTCTCGGGTGCCAAGGAACTCCAGT717390.12049383071049286RNA PCR Primer, Index 1 (100% over 30bp)
CGCCGCGACCGGCTCCGGGACGGTGGAATTCTCGGGTGCCAAGGAACTCC702400.11797608928344441RNA PCR Primer, Index 1 (100% over 27bp)
GACCATTCGTAGACGACCTGCTTTGGAATTCTCGGGTGCCAAGGAACTCC701080.11775438023182974RNA PCR Primer, Index 1 (100% over 27bp)
CCGAGCGCACGGGGTCGGCGGCTGGAATTCTCGGGTGCCAAGGAACTCCA688160.11558431890844975RNA PCR Primer, Index 1 (100% over 28bp)
ACGATGCCGACTGGCGATGCGGCGGCTGGAATTCTCGGGTGCCAAGGAAC683690.11483353143820914RNA PCR Primer, Index 1 (100% over 24bp)
CTCGCCCGTCACGTTGAACGCACGTTCTGGAATTCTCGGGTGCCAAGGAA679590.11414488968698175RNA PCR Primer, Index 1 (100% over 23bp)
GGGGAAGGTGGCTCGGGGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTC677590.11380896688150496RNA PCR Primer, Index 1 (100% over 31bp)
CGGTTGGCCCCGGATAGCCGTGGAATTCTCGGGTGCCAAGGAACTCCAGT660770.11098385608744527RNA PCR Primer, Index 1 (100% over 30bp)
CTAAACCATTCGTAGACGACCTGCTTCTGGAATTCTCGGGTGCCAAGGAA655920.11016924328416408RNA PCR Primer, Index 1 (100% over 23bp)
CCGGGGTCCGGTGCGGAGAGCCGTTGGAATTCTCGGGTGCCAAGGAACTC651850.10948564037501884RNA PCR Primer, Index 1 (100% over 26bp)
GCTAAACCATTCGTAGACGACCTGCTTTGGAATTCTCGGGTGCCAAGGAA645170.10836365820472642RNA PCR Primer, Index 1 (100% over 23bp)
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAG638170.10718792838555768No Hit
GGCCCGGTGGGGAAGGTGGCTCGGGGGTGGAATTCTCGGGTGCCAAGGAA637220.10702836505295621RNA PCR Primer, Index 1 (100% over 23bp)
TTTTCACTGACCCGGTGAGGCGGGGTGGAATTCTCGGGTGCCAAGGAACT628950.10563932425230974RNA PCR Primer, Index 1 (100% over 25bp)
TTTTTCACTGACCCGGTGAGGCGGGGTGGAATTCTCGGGTGCCAAGGAAC621360.10436449720552536RNA PCR Primer, Index 1 (100% over 24bp)
TGGTGGTGCATGGCCGTTCTTAGTTGGTGGAATTCTCGGGTGCCAAGGAA612000.10279237847589404RNA PCR Primer, Index 1 (100% over 23bp)
GGTCCGGTGCGGAGAGCCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTC611240.10266472780981287RNA PCR Primer, Index 1 (100% over 31bp)
CGAGCGCACGGGGTCGGCGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG605060.10162672634088962RNA PCR Primer, Index 1 (100% over 29bp)
GGGTCCGGTGCGGAGAGCCGTTGGAATTCTCGGGTGCCAAGGAACTCCAG600190.10080875430955369RNA PCR Primer, Index 1 (100% over 29bp)

[FAIL]Adapter Content

Adapter graph