FastQCFastQC Report
Thu 14 Sept 2023
SRR15175565.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR15175565.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20097195
Total Bases1 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCGAAAAAAAAAAAAA2280411.1346906869341717No Hit
GGGGGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCGCAAAAAAAAAAAA2236101.1126428339875292No Hit
CCGGGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCGAAAAAAAAAAAAA2198211.0937894566878612No Hit
CCGGGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCGCAAAAAAAAAAAA2171411.0804542623983098No Hit
GCGGGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCGAAAAAAAAAAAAA1617330.8047540962806004No Hit
GCGGGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCGCAAAAAAAAAAAA1567900.7801586241264018No Hit
CCGGGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCAAAAAAAAAAAAAA1484270.7385458517967308No Hit
GGGGGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCAAAAAAAAAAAAAA1478740.7357942240198196No Hit
CGGGGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCGAAAAAAAAAAAAA1444020.71851818126858No Hit
CGGGGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCGCAAAAAAAAAAAA1441640.7173339364025676No Hit
CGGGGGGGGTCTCCCCCGCGGGGGCGCGCCGGCAAAAAAAAAAAAAAAGA1222110.6080997870598359No Hit
GCGGGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCAAAAAAAAAAAAAA1077220.5360051489772578No Hit
CGGGGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCAAAAAAAAAAAAAA954620.47500161092132503No Hit
CCGCCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAAAAAAA793270.3947167751519553No Hit
CCGCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAAAAAAAA747480.3719325010281286No Hit
CGCCCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAAAAAAA695520.34607814672644616No Hit
CGGGGGGGGTCTCCCCCGCGGGGGCGCGCCGGCGAAAAAAAAAAAAAAAG673210.33497709506227114No Hit
GGGGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCGAAAAAAAAAAAAAA638190.3175517777480887No Hit
GGGGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCGCAAAAAAAAAAAAA637140.3170293167777891No Hit
GCCCCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAAAAAAA609900.3034751864625884No Hit
CCGCGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTAAAAA604320.3006986795918535No Hit
GCGCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAAAAAAAA543390.2703810158581832No Hit
CCCCCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAAAAAAA541410.2693958037427611No Hit
CCGCGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTCAAAA531010.2642209522274128No Hit
GGCCCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAAAAAAA522360.25991686899589717No Hit
GCGCCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAAAAAAA509380.25345825623924134No Hit
CGGCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAAAAAAAA496920.24725838605835293No Hit
GGGCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAAAAAAAA476170.23693356212148015No Hit
CGGGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCGCAAAAAAAAAAAAA433160.21553256561425613No Hit
CGGGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCGAAAAAAAAAAAAAA424940.21144244258962508No Hit
GGGCGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTAAAAA414630.20631237344315959No Hit
CCGCGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCAAAAAA401970.20001298688697602No Hit
GGGGGGCCCGGGGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTG397120.1975997147860684Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGGCGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTAAAAA390970.19453958624574225No Hit
GGGCGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTCAAAA360860.17955739594505601No Hit
GCGCGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTAAAAA356590.17743272133250437No Hit
CCGGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTAAAAAA349210.17376056708411297No Hit
CGGCGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTCAAAA338110.16823740825523165No Hit
GCCCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAAAAAAAA335570.1669735502889831No Hit
CGGCCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAAAAAAA331100.1647493593011363No Hit
GGGCCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAAAAAAA317990.15822606090053862No Hit
CCCCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAAAAAAAA315610.15704181603452622No Hit
GGGGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCAAAAAAAAAAAAAAA313890.15618597520698785No Hit
CCCCGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTAAAAA309990.15424540588873223No Hit
CGCCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAAAAAAAA306800.15265811970277443No Hit
GCGCGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTCAAAA305190.1518570128816484No Hit
CCGGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTCAAAAA295240.14690607321071425No Hit
CCCCGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTCAAAA270310.1345013570301726No Hit
GGGCGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCAAAAAA269090.13389430714087214No Hit
CCGCGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTCCAAA268040.13337184617057254No Hit
GCGGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTAAAAAA265860.13228711767985532No Hit
GCCGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTAAAAAA262810.13076949295660414No Hit
CGGCGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCAAAAAA251630.12520652757760473No Hit
CCCGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTAAAAAA246340.12257431945104777No Hit
CGGGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCAAAAAAAAAAAAAAA242770.12079795215202917No Hit
GGGGGGGGTCTCCCCCGCGGGGGCGCGCCGGCAAAAAAAAAAAAAAAGAT237880.11836477677606254No Hit
CGCGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTAAAAAA235870.11736463720434617No Hit
GCGCGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCAAAAAA233490.11618039233833377No Hit
GGGGGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCACAAAAAAAAAAAA231670.11527479332314784No Hit
CCGGGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCACAAAAAAAAAAAA229070.11398108044431077No Hit
CGGGGGGGGTCTCCCCCGCGGGGGCGCGCCGGCGAAAAAAAAAAAAAAAA226610.1127570290281803No Hit
GCGGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTCAAAAA225600.11225447133293975No Hit
CCGGGGCCCGGGGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTG220710.10982129595697311Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCCGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTCAAAAA219850.10939337554320393No Hit
CGGGGGGGGTCTCCCCCGCGGGGGCGCGCCGGCAAAAAAAAAAAAAAAAG217640.1082937195961924No Hit
GGCCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAAAAAAAA217040.10799517047030693No Hit
CCGCGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTAAAAAAA212380.1056764389259297No Hit
CCCGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTCAAAAA211720.1053480348874557No Hit
CCCCGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCAAAAAA201850.10043690176663957No Hit

[FAIL]Adapter Content

Adapter graph