FastQCFastQC Report
Thu 14 Sept 2023
SRR15217936_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR15217936_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18359018
Total Bases1.2 Gbp
Sequences flagged as poor quality0
Sequence length66
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCCGTCTT663720.3615226043135858TruSeq Adapter, Index 1 (100% over 48bp)
TCGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCCGTCTT660530.3597850386115423TruSeq Adapter, Index 1 (100% over 48bp)
CTGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCCGTCTT519880.2831741872032589TruSeq Adapter, Index 1 (100% over 48bp)
GCGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCCGTCTT467720.25476308155479777TruSeq Adapter, Index 1 (100% over 48bp)
TGGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCCGTCTT441410.2404322496987584TruSeq Adapter, Index 1 (100% over 48bp)
CCGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCCGTCTT436500.23775781471536223TruSeq Adapter, Index 1 (100% over 48bp)
GTGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCCGTCTT359810.19598542797877316TruSeq Adapter, Index 1 (100% over 48bp)
GGGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCCGTCTT269670.14688694133858357TruSeq Adapter, Index 1 (100% over 48bp)
ACGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCCGTCTT250930.1366794237033811TruSeq Adapter, Index 1 (98% over 50bp)
CGGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCCGTCTT231190.12592721462553172TruSeq Adapter, Index 1 (100% over 48bp)
ATGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCCGTCTT224310.12217973750012119TruSeq Adapter, Index 1 (98% over 50bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)