FastQCFastQC Report
Thu 14 Sept 2023
SRR15217938_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR15217938_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22879461
Total Bases1.5 Gbp
Sequences flagged as poor quality0
Sequence length66
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCCGTCTT833600.3643442474453397TruSeq Adapter, Index 2 (100% over 48bp)
TTGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCCGTCTT817820.35744723182071464TruSeq Adapter, Index 2 (100% over 48bp)
CTGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCCGTCTT645940.28232308444678833TruSeq Adapter, Index 2 (100% over 48bp)
GCGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCCGTCTT559270.24444194729937038TruSeq Adapter, Index 2 (100% over 48bp)
TGGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCCGTCTT544050.23778969268550515TruSeq Adapter, Index 2 (100% over 48bp)
CCGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCCGTCTT532360.23268030658589378TruSeq Adapter, Index 2 (100% over 48bp)
GTGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCCGTCTT436360.19072127616992376TruSeq Adapter, Index 2 (100% over 48bp)
GGGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCCGTCTT335380.1465856210511253TruSeq Adapter, Index 2 (100% over 48bp)
ACGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCCGTCTT310990.1359254048860679TruSeq Adapter, Index 2 (98% over 50bp)
ATGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCCGTCTT281880.123202203058892TruSeq Adapter, Index 2 (98% over 50bp)
CGGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCCGTCTT277440.12126159790215338TruSeq Adapter, Index 2 (100% over 48bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)