FastQCFastQC Report
Thu 14 Sept 2023
SRR15217940_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR15217940_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15091323
Total Bases996 Mbp
Sequences flagged as poor quality0
Sequence length66
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGCCGTCTT313030.20742382891148775TruSeq Adapter, Index 3 (100% over 48bp)
TTGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGCCGTCTT302080.2001680038257746TruSeq Adapter, Index 3 (100% over 48bp)
CTGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGCCGTCTT243800.16154978592665467TruSeq Adapter, Index 3 (100% over 48bp)
GCGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGCCGTCTT205980.1364890275027577TruSeq Adapter, Index 3 (100% over 48bp)
CCGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGCCGTCTT200560.13289755974343667TruSeq Adapter, Index 3 (100% over 48bp)
TGGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGCCGTCTT190340.12612545633010439TruSeq Adapter, Index 3 (100% over 48bp)
GTGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGCCGTCTT157820.10457664977417819TruSeq Adapter, Index 3 (100% over 48bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)