FastQCFastQC Report
Thu 14 Sept 2023
SRR15217942_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR15217942_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18367205
Total Bases1.2 Gbp
Sequences flagged as poor quality0
Sequence length66
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCGTCTT419580.2284397653317421TruSeq Adapter, Index 4 (100% over 48bp)
TCGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCGTCTT409890.22316405789558075TruSeq Adapter, Index 4 (100% over 48bp)
CTGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCGTCTT321010.17477346172158476TruSeq Adapter, Index 4 (100% over 48bp)
TGGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCGTCTT284820.15506986501212353TruSeq Adapter, Index 4 (100% over 48bp)
GCGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCGTCTT281300.15315340575770783TruSeq Adapter, Index 4 (100% over 48bp)
CCGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCGTCTT264320.14390866764975946TruSeq Adapter, Index 4 (100% over 48bp)
GTGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCGTCTT226230.1231706185018352TruSeq Adapter, Index 4 (100% over 48bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)