FastQCFastQC Report
Thu 14 Sept 2023
SRR15217944_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR15217944_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15904572
Total Bases1 Gbp
Sequences flagged as poor quality0
Sequence length66
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCCGTCTT317610.19969729458925398TruSeq Adapter, Index 5 (100% over 48bp)
TCGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCCGTCTT306770.19288164434729838TruSeq Adapter, Index 5 (100% over 48bp)
CTGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCCGTCTT246790.15516921800850725TruSeq Adapter, Index 5 (100% over 48bp)
TGGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCCGTCTT216230.13595461732638892TruSeq Adapter, Index 5 (100% over 48bp)
GCGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCCGTCTT208070.1308240171442526TruSeq Adapter, Index 5 (100% over 48bp)
CCGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCCGTCTT197420.12412782940653794TruSeq Adapter, Index 5 (100% over 48bp)
GTGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCCGTCTT172860.10868572885834338TruSeq Adapter, Index 5 (100% over 48bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)