FastQCFastQC Report
Thu 14 Sept 2023
SRR15228866_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR15228866_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51603259
Total Bases2.6 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGT18467513.578748776312752No Hit
CTAAACCATTCGTAGACGACCTGCTTAGATCGGAAGAGCACACGTCTGAA17231223.3391728223986785Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTC13677362.65048376111284No Hit
TTTCGCCGAATCCCGGGGCCGAGGAAGAGATCGGAAGAGCACACGTCTGA12489962.4203820150196327Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTTTCGCCGAATCCCGGGGCCGAGGAAGAGATCGGAAGAGCACACGTCTG12317552.3869713345042802Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATG7960971.5427262064979268TruSeq Adapter, Index 7 (100% over 49bp)
CCGCCTGGATACCGCAGCTAGGAATAATGGAATAGATCGGAAGAGCACAC7209291.3970609879503928No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCAGATCGGAAGAGCACACG6118711.1857216227370446No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAGATCGGAAGAGCACAC5970221.156946308371725No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAGATCGGAAGAGCACACG5373621.041333455315293No Hit
GTGGGAAATGTGGCGTACGGAAGACCCACTCCCCGGCGCAGATCGGAAGA5199131.0075196994825464No Hit
TTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAGATCGGAAGAGCACACGT5132090.9945282719449947No Hit
TTTTCGCCGAATCCCGGGGCCGAGGAAGAGATCGGAAGAGCACACGTCTG4788550.9279549572634551Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTC4747760.9200504177459025No Hit
CTAAACCATTCGTAGACGACCTGCTTCAGATCGGAAGAGCACACGTCTGA4735710.91771529391196Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTTTCGCCGAATCCCGGGGCCGAGGAAGCAGATCGGAAGAGCACACGTCT4345500.842097976796388Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTTCGCCGAATCCCGGGGCCGAGGAAGCAGATCGGAAGAGCACACGTCTG3732170.7232430804418768Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTTTCGCCGAATCCCGGGGCCGAGGAAAGATCGGAAGAGCACACGTCTGA3693490.715747429828027Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTGGGAAATGTGGCGTACGGAAGACCCACTCCCCGGCGAGATCGGAAGAG3652010.7077091778253772No Hit
CGCCTGGATACCGCAGCTAGGAATAATGGAATAGATCGGAAGAGCACACG3599320.6974985824054253No Hit
GCTTTCGCCGAATCCCGGGGCCGAGGAAGAGATCGGAAGAGCACACGTCT3348050.6488059213469444Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCGCCTGGATACCGCAGCTAGGAATAATGGAAAGATCGGAAGAGCACACG2913880.5646697624272142No Hit
TGGATAACTGTGGTAATTCTAGAGCTAATACAAGATCGGAAGAGCACACG2632290.5101015034728718No Hit
GCTTTCGCCGAATCCCGGGGCCGAGGAAGCAGATCGGAAGAGCACACGTC2245010.43505197995343664No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACG2238110.43371485510246555No Hit
CTAGACCATTCGTAGACGACCTGCTTAGATCGGAAGAGCACACGTCTGAA2214540.42914731412603224Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCAGATCGGAAGAGCACAC2140270.4147548122881154No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCACA2063330.39984490126873573No Hit
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAGATCGGAAGAGCACACG1971040.3819603719214711No Hit
GCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGAGATCGGAAGAGCAC1877230.3637812875345722No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAGCCAAGATCGGAAGAGCACACGT1849270.3583630250949848No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACAC1822990.3532703234886774No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGATCGGAAGAGCACACGTC1604490.310928036541258No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCAAGATCGGAAGAGCACAC1579340.3060543133525733No Hit
TGGGAAATGTGGCGTACGGAAGACCCACTCCCCGGCGAGATCGGAAGAGC1533990.29726610871611814No Hit
GACGTACGCAGTTTTATCCGGTAAAGCAGATCGGAAGAGCACACGTCTGA1526090.2957351976548613Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACGTACGCAGTTTTATCCGGTAAAGCAGATCGGAAGAGCACACGTCTGAA1518250.29421591376622164Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGCCCGTGGACGGTGTGAGGCCGGTAGCAGATCGGAAGAGCACACGTCTG1467580.2843967664910466Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAGATCGGAAGAGCACACG1423110.27577909371964276No Hit
TTTCGCCGAATCCCGGGGCCGAGGAAGCCAAGATCGGAAGAGCACACGTC1417040.27460281142320875No Hit
TGGGAAATGTGGCGTACGGAAGACCCACTCCCCGGCGCAGATCGGAAGAG1396210.2705662446629582No Hit
TTTCGCCGAATCCCGGGGCCGAGGAAAGATCGGAAGAGCACACGTCTGAA1384460.2682892566921015Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGAGATCGGAAGAGCACACGTC1383710.2681439170343873No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTCT1371750.26582623395937066Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCAGATCGGAAGAGCACAC1371540.26578553885521067No Hit
TTTTCGCCGAATCCCGGGGCCGAGGAAGCAGATCGGAAGAGCACACGTCT1273080.2467053485904834Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT1216610.23576224129565151No Hit
TGGGGGGGACGCGTGCATTTATCAGAAGATCGGAAGAGCACACGTCTGAA1183650.22937504780463575Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGT1166990.22614656954127646No Hit
GGGAAATGTGGCGTACGGAAGACCCACTCCCCGGCGCAGATCGGAAGAGC1153560.22354402073713986No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCAGATCGGAAGAGCAC1147660.22240068209645444No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACACG1137580.22044731709677484No Hit
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCACAC1006780.19510008079140895No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAAGATCGGAAGAGCACAC994650.19274945406064373No Hit
GTCGCGGCGTGGGAAATGTGGCGTACAGATCGGAAGAGCACACGTCTGAA954250.1849204911651026Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGAGATCGGAAGAGCA930720.1803607016370807No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGT893690.17318479827020228No Hit
CTCGCTTCTGGCGCCAAGCGCCCGTCCGAGATCGGAAGAGCACACGTCTG878860.17031094877166575Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGCAGATCGGAAGAGCACACGT796250.15430226993996637No Hit
AGTTCTACAGTCCGACGATCAGATCGGAAGAGCACACGTCTGAACTCCAG794250.15391469751939504Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GCTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAGATCGGAAGAGCACAC779420.15104084802085854No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGCGTCCGGAGATCGGAAGAGC752230.14577180096319112No Hit
CGCCTGGATACCGCAGCTAGGAATAATGGAAAGATCGGAAGAGCACACGT751940.14571560296220826No Hit
GCCGCCGGTGAAATACCACTACTCTCATAGATCGGAAGAGCACACGTCTG749270.14519819378074553Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCTTTCGCCGAATCCCGGGGCCGAGGAAAGATCGGAAGAGCACACGTCTG717690.13907842525992398Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACG693500.1343907368331136No Hit
TTCTACAGTCCGACGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTC692640.1342240806922679Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
AGAGTTCTACAGTCCGACGATCAGATCGGAAGAGCACACGTCTGAACTCC680640.13189864616883984Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGATCGGAAGAGCACACGT672640.13034835648655446No Hit
TTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAAGATCGGAAGAGCACACG665040.12887558128838336No Hit
TTTTCGCCGAATCCCGGGGCCGAGGAAAGATCGGAAGAGCACACGTCTGA599610.11619614954939182Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGAGATCGGAAGAGCACA585880.11353546488216955No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAGATCGGAAGAGCACACGTC577660.11194254223362134No Hit
ACCCGTCGCCGCGCTCTCCCTCTCCCCAGATCGGAAGAGCACACGTCTGA566290.10973919302267324Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTGGGGGGGACGCGTGCATTTATCAGAAGATCGGAAGAGCACACGTCTGA547540.10610570157981687Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TACCCGTCGCCGCGCTCTCCCTCTCCCCAGATCGGAAGAGCACACGTCTG522380.10123004052902938Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACAC518710.10051884513728096No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)