FastQCFastQC Report
Thu 14 Sept 2023
SRR15228867_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR15228867_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38905512
Total Bases1.9 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATG23282525.984375684350331TruSeq Adapter, Index 8 (100% over 49bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTC15056033.869896378693076No Hit
TTTCGCCGAATCCCGGGGCCGAGGAAGAGATCGGAAGAGCACACGTCTGA13571823.4884054475365858Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTAAACCATTCGTAGACGACCTGCTTAGATCGGAAGAGCACACGTCTGAA12230263.1435802721218526Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CTTTCGCCGAATCCCGGGGCCGAGGAAGAGATCGGAAGAGCACACGTCTG7248481.8630984730389872Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGT6683741.7179416633817852No Hit
TTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAGATCGGAAGAGCACACGT6276531.6132752603281508No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCAGATCGGAAGAGCACACG5323241.3682482831738598No Hit
GTGGGAAATGTGGCGTACGGAAGACCCACTCCCCGGCGAGATCGGAAGAG5317521.3667780544823571No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAGATCGGAAGAGCACAC4882841.255050955247678No Hit
GTGGGAAATGTGGCGTACGGAAGACCCACTCCCCGGCGCAGATCGGAAGA4390321.1284570679856367No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAATAGATCGGAAGAGCACAC4376191.1248251918648442No Hit
TTTCGCCGAATCCCGGGGCCGAGGAAGCAGATCGGAAGAGCACACGTCTG3028540.7784346855530394Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAGATCGGAAGAGCACACG2802210.7202604093733556No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAAGATCGGAAGAGCACACGTCTGA2773950.7129966571317709Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGGGAAATGTGGCGTACGGAAGACCCACTCCCCGGCGAGATCGGAAGAGC2675930.6878022836455667No Hit
TTTCGCCGAATCCCGGGGCCGAGGAAGCCAGATCGGAAGAGCACACGTCT2551860.655912200821313Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTTTCGCCGAATCCCGGGGCCGAGGAAGAGATCGGAAGAGCACACGTCTG2531050.650563344340514Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCAGATCGGAAGAGCAC2383460.6126278456379137No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCAAGATCGGAAGAGCACAC2290730.5887931766583614No Hit
TTTCGCCGAATCCCGGGGCCGAGGAAAGATCGGAAGAGCACACGTCTGAA2282250.58661353691991Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CTTTCGCCGAATCCCGGGGCCGAGGAAGCAGATCGGAAGAGCACACGTCT2174360.5588822478418997Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGATCGGAAGAGCACACGTC2148470.552227663781934No Hit
GCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGAGATCGGAAGAGCAC2005540.5154899388035299No Hit
CTAGACCATTCGTAGACGACCTGCTTAGATCGGAAGAGCACACGTCTGAA1908220.49047548840894317Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTTCGCCGAATCCCGGGGCCGAGGAAGCCAAGATCGGAAGAGCACACGTC1895080.4870980749462956No Hit
CTAAACCATTCGTAGACGACCTGCTTCAGATCGGAAGAGCACACGTCTGA1761990.4528895545700568Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCGCCTGGATACCGCAGCTAGGAATAATGGAAAGATCGGAAGAGCACACG1755480.4512162698180145No Hit
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAGATCGGAAGAGCACACG1669590.4291397064765527No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCAGATCGGAAGAGCACAC1633810.4199430661650206No Hit
TGGATAACTGTGGTAATTCTAGAGCTAATACAAGATCGGAAGAGCACACG1387830.3567180917706468No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCACA1387620.3566641148431616No Hit
CGCCTGGATACCGCAGCTAGGAATAATGGAATAGATCGGAAGAGCACACG1361140.34985788132026124No Hit
TTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAAGATCGGAAGAGCACACG1314470.33786215176913753No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACAC1247390.3206203789324248No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTCT1105800.2842270781579741Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACGTACGCAGTTTTATCCGGTAAAGCAGATCGGAAGAGCACACGTCTGAA990530.25459888562833977Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGT956840.24593944426177966No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTC930680.23921546129504734No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGCC926580.2381616260441451TruSeq Adapter, Index 8 (100% over 50bp)
GTCGCGGCGTGGGAAATGTGGCGTACGAGATCGGAAGAGCACACGTCTGA855880.21998939379078214Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGGGAAATGTGGCGTACGGAAGACCCACTCCCCGGCGCAGATCGGAAGAG852650.21915917723946157No Hit
GCTTTCGCCGAATCCCGGGGCCGAGGAAGAGATCGGAAGAGCACACGTCT849370.2183161090387398Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCCGCCGGTGAAATACCACTACTCTCATAGATCGGAAGAGCACACGTCTG785590.2019225450625094Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCACAC783290.20133136919005204No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCAGATCGGAAGAGCACACGTCTG776570.19960410751052446Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAGATCGGAAGAGCACACG751360.19312430588241586No Hit
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGAGATCGGAAGAGCACACGTC745180.19153584201642174No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCAGATCGGAAGAGCACAC662280.17022780730915454No Hit
CTCGCTTCTGGCGCCAAGCGCCCGTCCGAGATCGGAAGAGCACACGTCTG640950.16474529367458268Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCAGATCGGAAGAGCACA600330.15430461370100976No Hit
GCTTTCGCCGAATCCCGGGGCCGAGGAAGCAGATCGGAAGAGCACACGTC590190.1516982992024369No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGAGATCGGAAGAGCA589780.1515929156773467No Hit
TCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGCCG582080.1496137616695547TruSeq Adapter, Index 8 (100% over 50bp)
TTTTCGCCGAATCCCGGGGCCGAGGAAAGATCGGAAGAGCACACGTCTGA581740.1495263704536262Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTCGAGATCGGAAGAGC581190.14938500231021248No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT577300.1483851439867955No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAAGATCGGAAGAGCACAC574840.14775284283625414No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACG566160.14552179650019773No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACG548550.1409954455810786No Hit
GTCGCGGCGTGGGAAATGTGGCGTAAGATCGGAAGAGCACACGTCTGAAC546590.1404916609245497Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GTCGCGGCGTGGGAAATGTGGCGTACAGATCGGAAGAGCACACGTCTGAA537090.13804984753831281Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCCGCCGGTGAAATACCACTACTCTCAAGATCGGAAGAGCACACGTCTGA505690.12997901171432985Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACGTACGCAGTTTTATCCGGTAAAGCGAGATCGGAAGAGCACACGTCTGA500950.12876067535109165Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCGCCTGGATACCGCAGCTAGGAATAATGGAGATCGGAAGAGCACACGTC496270.12755776096713495No Hit
TTTTCGCCGAATCCCGGGGCCGAGGAAGCAGATCGGAAGAGCACACGTCT483710.12432942663754175Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTCAGATCGGAAGAGCA473690.12175395609753188No Hit
TAAACCATTCGTAGACGACCTGCTTAGATCGGAAGAGCACACGTCTGAAC462350.11883920201332912Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AACGATGCCGACTGGCGATGCGGCGGCGAGATCGGAAGAGCACACGTCTG460830.11844851187153121Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGT451400.11602469079445607No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACACG448420.11525873249014176No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAGCCAAGATCGGAAGAGCACACGT435790.11201240585138682No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGAGATCGGAAGAGCACA415160.10670981530843239No Hit
GGGAAATGTGGCGTACGGAAGACCCACTCCCCGGCGCAGATCGGAAGAGC393610.10117075441649502No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)