FastQCFastQC Report
Thu 14 Sept 2023
SRR15228869_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR15228869_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19992602
Total Bases1.6 Gbp
Sequences flagged as poor quality0
Sequence length85
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATG455449922.780921662923113TruSeq Adapter, Index 9 (100% over 49bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACG9880534.942093080230378No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT4369162.1853883751599716No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACAC3369391.685318399275892No Hit
CCGTGGCGCAATGAAGGTGAAGGGCCAGATCGGAAGAGCACACGTCTGAA2883671.4423685321200312Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCAGATCGGAAGAGCACAC2677171.3390803258125181No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACG2427801.214349187764554No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGT2326391.163625425044724No Hit
GTGGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACG2177841.0893229405557114No Hit
CTCGCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCACACGTCT1484540.7425446672724241Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACACG1391690.6961024883104261No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACAC1176750.5885927204472934No Hit
GCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTCTGAACT1154110.5772685316298499Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CCGTGGCGCAATGAAGGTGAAGGGCCCAGATCGGAAGAGCACACGTCTGA1042250.5213178354673393Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACA1016980.508678160051403No Hit
GGATGCGTGCATTTATCAGATAGATCGGAAGAGCACACGTCTGAACTCCA907440.45388789313166944Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGAGATCGGAAGAGCACACGTC899070.4497013445273407No Hit
GGGATGCGTGCATTTATCAGATAGATCGGAAGAGCACACGTCTGAACTCC856300.4283084312887337Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGGGATGCGTGCATTTATCAGATAGATCGGAAGAGCACACGTCTGAACTC780270.3902793643368682Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGGGGGGGGATGCGTGCATTTATCAGAAGATCGGAAGAGCACACGTCTGA770560.38542256780783213Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGGTGGGGGGCGGGGCGGACTGTAGATCGGAAGAGCACACGTCTGAACTC745650.37296295899853354Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGGGATGCGTGCATTTATCAGAAGATCGGAAGAGCACACGTCTGAACTCC709350.3548062428292225Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GTGGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACAC687370.34381217612394827No Hit
CCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTCTGAACTC671160.33570417697506305Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CTCGCCCGTCACGTTGAACGCACGTTCGTAGATCGGAAGAGCACACGTCT663770.3320078096888039Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTCGCCCGTCACGTTGAACGCACGTTCGAGATCGGAAGAGCACACGTCTG663580.3319127745353006Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGGATGCGTGCATTTATCAGAAGATCGGAAGAGCACACGTCTGAACTCCA652370.326305700478607Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGGCCCGGTGGGGGGCGGGGCGGACTGTAGATCGGAAGAGCACACGTCTG590990.2956043440468629Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTCGCCCGTCACGTTGAACGCACGTTCGTGAGATCGGAAGAGCACACGTC542480.27134036880242No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCAGATCGGAAGAGCACACGTCTG529600.26489798576493445Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCAGATCGGAAGAGCACACG529030.2646128803044246No Hit
GCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACG521850.26102155187203746No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGT508870.25452915033270807No Hit
TGCGTGCATTTATCAGATCAAAACCAAAGATCGGAAGAGCACACGTCTGA506130.2531586433821871Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTCTGAACTCCAG504580.25238335660360767Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CCCCGCCCCTTGCCTCTCGGCGCCCCAGATCGGAAGAGCACACGTCTGAA489420.24480055172408274Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGGGCCCGGTGGGGGGCGGGGCGGACTGTAGATCGGAAGAGCACACGTCT478230.23920348136775793Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC465970.23307121304170414TruSeq Adapter, Index 9 (100% over 50bp)
GCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTC460620.23039522319305913Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGAGATCGGAAGAGCACACGT457840.2290047088418006No Hit
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCAGATCGGAAGAGCAC457700.22893468293921923No Hit
CCGTGGCGCAATGAAGGTGAAGGGCCCCAGATCGGAAGAGCACACGTCTG434730.217445433065691Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGTGGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACAC431490.21582483360595087No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCAGATCGGAAGAGCACACG421750.2109530315263616No Hit
CGGGGGGGGATGCGTGCATTTATCAGATAGATCGGAAGAGCACACGTCTG419770.2099626651898537Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTCTGAAC407970.20406048197228155Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ATGCGTGCATTTATCAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTC402960.20155455502990555Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CTCGCTTCTGGCGCCAAGCGTCCGTCCCAGATCGGAAGAGCACACGTCTG399780.19996396667127167Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTAGATCGGAAGAGCACACGT381120.19063051422721264No Hit
GGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTCTGAA377200.18866978895493441Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATGCGTGCATTTATCAGATCAAAACCAAAGATCGGAAGAGCACACGTCTG372730.18643396192251513Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACACGTCTGAAC370820.18547860853729795Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACACGTCTGAACTCCA370250.18519350307678809Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTCTGAACTCCA361520.18082688786582157Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGTC346210.1731690552335309No Hit
GGATGCGTGCATTTATCAGAAGATCGGAAGAGCACACGTCTGAACTCCAG325620.16287024570388586Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCTCGCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCACACGTC324590.1623550551348944No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCAGATCGGAAGAGCACA317160.15863868044789767No Hit
ACCGCCCGTCCCCGCCCCTTGCCTCTCGGCAGATCGGAAGAGCACACGTC308760.1544371262930158No Hit
ATGCGTGCATTTATCAGATAGATCGGAAGAGCACACGTCTGAACTCCAGT308680.15439711149154073Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCC304970.15254142507313456TruSeq Adapter, Index 9 (100% over 50bp)
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCCGAGATCGGAAGAGCAC297640.1488750688879817No Hit
ATGCGTGCATTTATCAGATCAAAACCAAGATCGGAAGAGCACACGTCTGA292780.14644416969837143Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTCTGAACTCC282210.14115721405347836Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC278300.13920149063138457TruSeq Adapter, Index 9 (98% over 50bp)
CCGTGGCGCAATGAAGGTGAAGGAGATCGGAAGAGCACACGTCTGAACTC277870.13898641107345605Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CCCCGCCCCTTGCCTCTCGGCGAGATCGGAAGAGCACACGTCTGAACTCC272990.13654550818347708Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTCTGA269370.13473483841673034Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCC262070.1310834877821306Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGATGCGTGCATTTATCAGATCAGATCGGAAGAGCACACGTCTGAACTCC258520.1293078309666746Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CTCCCGGGGCTACGCCTGTCTGAGAGATCGGAAGAGCACACGTCTGAACT252270.1261816746014351Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCCCGTGGATCGCCTCAGCTGCGGCGGGCGTCGCGAGATCGGAAGAGCAC235430.11775855889093376No Hit
GCTTCTGGCGCCAAGCGTCCGTCCAGATCGGAAGAGCACACGTCTGAACT226520.11330191037664832Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GATGCGTGCATTTATCAGAAGATCGGAAGAGCACACGTCTGAACTCCAGT226310.11319687152277627Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TGCGTGCATTTATCAGATCAAAACCAACAGATCGGAAGAGCACACGTCTG225430.11275670870655055Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGTGGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACA224350.11221650888663716No Hit
GATGCGTGCATTTATCAGATAGATCGGAAGAGCACACGTCTGAACTCCAG223560.1118213627220709Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCCCGTGGATCGCCTCAGCTGCGGCGGGCGTCAGATCGGAAGAGCACACG221990.11103607224312274No Hit
TGCGTGCATTTATCAGATCAAAACCAACCAGATCGGAAGAGCACACGTCT221400.11074096308224413Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGTGGGGGGCGGGGCGGACTGTAGATCGGAAGAGCACACGTCTGAACTCC213030.10655441447791539Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GTGCATTTATCAGATCAAAACCAACCAGATCGGAAGAGCACACGTCTGAA210120.10509887607425988Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACACGTCTGAACT209850.10496382611928153Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACACGTCTGAA209490.10478375951264374Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTC200680.10037712950020211No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)