FastQCFastQC Report
Thu 14 Sept 2023
SRR15228870_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR15228870_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37923233
Total Bases1.9 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTC20447095.391705396003553No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAGAGATCGGAAGAGCACACGTCTG11439713.0165439745076585Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAGATCGGAAGAGCACAC8506462.243073526985423No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGT7909852.0857530791217087No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATG5823561.5356180207526084TruSeq Adapter, Index 3 (100% over 49bp)
GTGGGAAATGTGGCGTACGGAAGACCCACTCCCCGGCGAGATCGGAAGAG5669041.4948725495001969No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACG5341941.4086193547897143No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGT4771721.2582577018156655No Hit
TTTCGCCGAATCCCGGGGCCGAGGAAGAGATCGGAAGAGCACACGTCTGA4522751.1926066535519269Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGCCTGGATACCGCAGCTAGGAATAATGGAATAGATCGGAAGAGCACACG3962261.0448107100995319No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAGCAGATCGGAAGAGCACACGTCT3846101.0141804101986769Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTAAACCATTCGTAGACGACCTGCTTAGATCGGAAGAGCACACGTCTGAA3668520.9673542337490055Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAGATCGGAAGAGCACACGT3415320.9005877742543734No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTC3120040.8227252143824341No Hit
GCTTTCGCCGAATCCCGGGGCCGAGGAAGAGATCGGAAGAGCACACGTCT2995370.7898509074898757Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGATCGGAAGAGCACACGTC2854690.7527549141182135No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT2790470.735820703894101No Hit
TTTCGCCGAATCCCGGGGCCGAGGAAGCAGATCGGAAGAGCACACGTCTG2577970.6797864517510941Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTGGTGACTCTAGATAACCTCGGGCCGATCGCAGATCGGAAGAGCACACG2560140.6750848483830479No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGT2554590.6736213655623717No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAGATCGGAAGAGCACACG2458990.6484125443629767No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAAAGATCGGAAGAGCACACG2257450.5952683411775573No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACAC2225930.5869568135185099No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAAGATCGGAAGAGCACACGTCTGA2123120.5598467831052273Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCGCCTGGATACCGCAGCTAGGAATAATGGAATAGATCGGAAGAGCACAC2072140.5464038364028722No Hit
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAGATCGGAAGAGCACACG1982960.5228879088446916No Hit
TGGGAAATGTGGCGTACGGAAGACCCACTCCCCGGCGAGATCGGAAGAGC1940720.5117496179716534No Hit
CGCCTGGATACCGCAGCTAGGAATAATGGAAAGATCGGAAGAGCACACGT1835660.48404628371215075No Hit
TTTCGCCGAATCCCGGGGCCGAGGAAGCCAGATCGGAAGAGCACACGTCT1772060.46727556165899675Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGAGATCGGAAGAGCAC1617360.42648262609888776No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTCT1566750.41313724491791093Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCAGATCGGAAGAGCAC1405680.3706646002465033No Hit
CTAAACCATTCGTAGACGACCTGCTTCAGATCGGAAGAGCACACGTCTGA1399960.36915629002411265Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCTTTCGCCGAATCCCGGGGCCGAGGAAAGATCGGAAGAGCACACGTCTG1261820.33273007077218336Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGTC1185800.3126843114878945No Hit
TTTCGCCGAATCCCGGGGCCGAGGAAGCCAAGATCGGAAGAGCACACGTC1181290.31149506688947115No Hit
GTCGCGGCGTGGGAAATGTGGCGTACGAGATCGGAAGAGCACACGTCTGA1178890.31086220945350307Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTTTCGCCGAATCCCGGGGCCGAGGAAGAGATCGGAAGAGCACACGTCTG1162550.3065535050769537Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGGATAACTGTGGTAATTCTAGAGCTAATACAAGATCGGAAGAGCACACG1161010.30614742155554087No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCACA1147650.3026245151619853No Hit
TTTCGCCGAATCCCGGGGCCGAGGAAAGATCGGAAGAGCACACGTCTGAA1095780.2889468838271252Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCACAC1080310.2848675902711143No Hit
GCTTTCGCCGAATCCCGGGGCCGAGGAAGCAGATCGGAAGAGCACACGTC1043820.27524552033841626No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGT1017610.26833418975644824No Hit
GTGGGAAATGTGGCGTACGGAAGACCCACTCCCCGGCGCAGATCGGAAGA999730.2636194018584861No Hit
GGAATGAGTCCACTTTAAATCCTTTAACGAAGATCGGAAGAGCACACGTC949530.25038213382282043No Hit
GCCGCCGGTGAAATACCACTACTCTCATAGATCGGAAGAGCACACGTCTG943820.24887646050641304Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTTTCGCCGAATCCCGGGGCCGAGGAAGCCAAGATCGGAAGAGCACACGT920970.2428511303348003No Hit
TTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAAGATCGGAAGAGCACACG846350.22317453788815947No Hit
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGAGATCGGAAGAGCACACGTC843730.222483668520561No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGAGATCGGAAGAGCACACGTC842340.22211713858889615No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAGATCGGAAGAGCACACGTC823740.21721249346014354No Hit
GCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCAGATCGGAAGAGCACA804290.2120837113228189No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGAAGATCGGAAGAGCACACGTC790550.20846060250190165No Hit
GGGCTCTCGCTTCTGGCGCCAAGCGTCCGAGATCGGAAGAGCACACGTCT778710.20533850581779248Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGATCGGAAGAGCACACGT766780.2021926769798345No Hit
GGCCGCCGGTGAAATACCACTACTCTCATAGATCGGAAGAGCACACGTCT752650.19846672882557242Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGCCGCCGGTGAAATACCACTACTCTCAAGATCGGAAGAGCACACGTCTG709730.18714912834567665Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTGCGCCGCGACCGGCTCCGGGACGGCCGGGAGATCGGAAGAGCACACGT702400.1852162762599908No Hit
GTCGCGGCGTGGGAAATGTGGCGTACAGATCGGAAGAGCACACGTCTGAA685090.18065179200307105Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAGATCGGAAGAGCACACG671450.1770550522419858No Hit
GACGTACGCAGTTTTATCCGGTAAAGCAGATCGGAAGAGCACACGTCTGA641510.16916015572828402Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTTTCGCCGAATCCCGGGGCCGAGGAAGCAGATCGGAAGAGCACACGTCT628400.1657031719843084Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTCAGATCGGAAGAGCA612630.16154477124880148No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCAAGATCGGAAGAGCACAC520920.13736170647687132No Hit
GGGAAATGTGGCGTACGGAAGACCCACTCCCCGGCGCAGATCGGAAGAGC510910.1347221635876878No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAAGATCGGAAGAGCACAC510480.13460877663041018No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTAGATCGGAAGAGCACACGT494880.13049520329661768No Hit
GTCGCGGCGTGGGAAATGTGGCGTAAGATCGGAAGAGCACACGTCTGAAC477530.12592017141576511Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGAGATCGGAAGAGCACA476940.12576459396275627No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCAGATCGGAAGAGCACAC475030.125260944919965No Hit
GCTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAGATCGGAAGAGCACAC451370.11902202536371305No Hit
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGAGATCGGAAGAGCACACGT443590.11697051250878321No Hit
CGTCGCGGCGTGGGAAATGTGGCGTACGAGATCGGAAGAGCACACGTCTG432590.11406991592726286Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGGCCGCCGGTGAAATACCACTACTCTCATAGATCGGAAGAGCACACGTC432570.11406464211529645No Hit
CGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCAGATCGGAAGAGCACAC432330.11400135637169963No Hit
CTAGACCATTCGTAGACGACCTGCTTAGATCGGAAGAGCACACGTCTGAA420060.11076587273031284Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TGTGGGAAATGTGGCGTACGGAAGACCCACTCCCCGGCGAGATCGGAAGA418420.110333420149068No Hit
AGCTTTCGCCGAATCCCGGGGCCGAGGAAAGATCGGAAGAGCACACGTCT408430.10769915107185088Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGCAGATCGGAAGAGCACACGT390650.10301073223372068No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACAAGATCGGAAGAGCACACG385070.1015393386950949No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCAGATCGGAAGAGCACACGTCTG382200.1007825466779164Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)