FastQCFastQC Report
Thu 14 Sept 2023
SRR15228871_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR15228871_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41091998
Total Bases2 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAAACCATTCGTAGACGACCTGCTTAGATCGGAAGAGCACACGTCTGAA10777302.6227247455818525Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATG9146232.2257934501018912TruSeq Adapter, Index 1 (100% over 49bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTC8579642.0879101571065006No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAGAGATCGGAAGAGCACACGTCTG7400161.8008761705867893Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTGGGAAATGTGGCGTACGGAAGACCCACTCCCCGGCGAGATCGGAAGAG7303251.7772925035185683No Hit
TTTCGCCGAATCCCGGGGCCGAGGAAGAGATCGGAAGAGCACACGTCTGA6895631.6780955747150577Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGT6306841.5348097700189707No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT5663971.3783632521348803No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAGATCGGAAGAGCACAC5539551.3480848509726882No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGT4592141.1175265802358894No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACG4433071.078815880405718No Hit
TTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAGATCGGAAGAGCACACGT3866690.9409836922507395No Hit
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAGATCGGAAGAGCACACG3299930.8030590286702536No Hit
TTTTCGCCGAATCCCGGGGCCGAGGAAGAGATCGGAAGAGCACACGTCTG3276420.7973377201079392Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACAC3115090.7580770348523818No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAGCAGATCGGAAGAGCACACGTCT2995970.7290884225196351Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCAGATCGGAAGAGCACAC2813520.6846880504569284No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAGATCGGAAGAGCACACG2671600.6501509125937366No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGATCGGAAGAGCACACGTC2657750.646780426690374No Hit
TTTCGCCGAATCCCGGGGCCGAGGAAGCAGATCGGAAGAGCACACGTCTG2469940.601075664415247Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTTTCGCCGAATCCCGGGGCCGAGGAAAGATCGGAAGAGCACACGTCTGA2279740.5547892803849547Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGTC2169870.528051714594165No Hit
TTTCGCCGAATCCCGGGGCCGAGGAAGCCAAGATCGGAAGAGCACACGTC2094760.509773216673475No Hit
TTTCGCCGAATCCCGGGGCCGAGGAAGCCAGATCGGAAGAGCACACGTCT1988530.48392146811649317Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCCGCCGGTGAAATACCACTACTCTCATAGATCGGAAGAGCACACGTCTG1948990.47429915673606327Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGT1810430.4405796963194634No Hit
CTAAACCATTCGTAGACGACCTGCTTCAGATCGGAAGAGCACACGTCTGA1798070.4375718114266432Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTTTCGCCGAATCCCGGGGCCGAGGAAGCCAAGATCGGAAGAGCACACGT1601370.3897036109074083No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAAAGATCGGAAGAGCACACG1500950.36526576293515833No Hit
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCACAC1465040.35652683522470724No Hit
GCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGAGATCGGAAGAGCAC1451940.3533388666085305No Hit
GCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCAGATCGGAAGAGCACA1421610.34595786751474095No Hit
TGGGAAATGTGGCGTACGGAAGACCCACTCCCCGGCGAGATCGGAAGAGC1215400.29577534779399145No Hit
ACGTACGCAGTTTTATCCGGTAAAGCAGATCGGAAGAGCACACGTCTGAA1066430.25952254743125414Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CCGCCTGGATACCGCAGCTAGGAATAATGGAGATCGGAAGAGCACACGTC1035590.25201743658217834No Hit
GCCGCCGGTGAAATACCACTACTCTCAAGATCGGAAGAGCACACGTCTGA993070.24166992318066402Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGCCCGTGGACGGTGTGAGGCCGGTAGCAGATCGGAAGAGCACACGTCTG979020.23825076600071868Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTCGCGGCGTGGGAAATGTGGCGTACGAGATCGGAAGAGCACACGTCTGA933090.22707340733346673Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCTTTCGCCGAATCCCGGGGCCGAGGAAGAGATCGGAAGAGCACACGTCT876740.2133602751562482Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGCCTGGATACCGCAGCTAGGAATAATGGAATAGATCGGAAGAGCACACG866620.21089750856115586No Hit
CGCCTGGATACCGCAGCTAGGAATAATGGAAAGATCGGAAGAGCACACGT853460.20769493856200422No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCAGATCGGAAGAGCAC850440.20696000228560313No Hit
TTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAGATCGGAAGAGCACACG849460.2067215130303472No Hit
TTTTCGCCGAATCCCGGGGCCGAGGAAGCAGATCGGAAGAGCACACGTCT832320.20255038462719677Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTGGTGACTCTAGATAACCTCGGGCCGATCGCAGATCGGAAGAGCACACG815790.19852770361762406No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCCGGGAGATCGGAAGAGCACACGT807930.19661492244791795No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTC806400.19624258718205911No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAATAGATCGGAAGAGCACAC780480.18993478973692152No Hit
TTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAAGATCGGAAGAGCACACG776810.1890416718116262No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGAGATCGGAAGAGCACA747200.18183588931353498No Hit
GCTTTCGCCGAATCCCGGGGCCGAGGAAGCAGATCGGAAGAGCACACGTC745050.1813126730902693No Hit
CTAGACCATTCGTAGACGACCTGCTTAGATCGGAAGAGCACACGTCTGAA741140.18036114963307454Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGCTGCGATCTATTGAAAGTCAGCCCTCGAAGATCGGAAGAGCACACGTC736240.17916870335679466No Hit
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGAGATCGGAAGAGCACACGTC686620.1670933596365891No Hit
GAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTCAGATCGGAAGAGCA681550.1658595427752138No Hit
TTTCGCCGAATCCCGGGGCCGAGGAAAGATCGGAAGAGCACACGTCTGAA678590.1651392078817876Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACGTACGCAGTTTTATCCGGTAAAGCGAGATCGGAAGAGCACACGTCTGA678500.1651173058073253Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTGGGAAATGTGGCGTACGGAAGACCCACTCCCCGGCGCAGATCGGAAGA639670.1556677774587646No Hit
GTCGCGGCGTGGGAAATGTGGCGTAAGATCGGAAGAGCACACGTCTGAAC608080.14798014932250314Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGGAAATGTGGCGTACGGAAGACCCACTCCCCGGCGCAGATCGGAAGAGC601520.14638373145058559No Hit
GTCGCGGCGTGGGAAATGTGGCGTACAGATCGGAAGAGCACACGTCTGAA561060.13653753219787462Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTCT553590.1347196600175051Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGT541250.13171664225234314No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGCGAGATCGGAAGAGCACACG523820.12747494049814762No Hit
AGCCGACTTAGAACTGGTGCGGACCAGGAGATCGGAAGAGCACACGTCTG522860.1272413183705499Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GACGTACGCAGTTTTATCCGGTAAAGCAGATCGGAAGAGCACACGTCTGA515310.12540397767954725Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGCCGCCGGTGAAATACCACTACTCTCATAGATCGGAAGAGCACACGTCT512400.12469581060526676Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCCCGTGGACGGTGTGAGGCCGGTAGCGAGATCGGAAGAGCACACGTCT493950.12020588534049867Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCCGACTTAGAACTGGTGCGGACCAGGGGAAGATCGGAAGAGCACACGT478430.11642899427766935No Hit
TTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGATCGGAAGAGCACACGTC470840.1145819193313501No Hit
GCTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGATCGGAAGAGCACACGT469780.11432396156546099No Hit
TTTTCGCCGAATCCCGGGGCCGAGGAAGCCAAGATCGGAAGAGCACACGT467360.11373503911880849No Hit
GGGAAATGTGGCGTACGGAAGACCCACTCCCCGGCGAGATCGGAAGAGCA446770.10872433119460388No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGCGTCCGGAGATCGGAAGAGC444410.10815001013092622No Hit
AGCCGACTTAGAACTGGTGCGGACCAGGGGAGATCGGAAGAGCACACGTC440620.10722768943968118No Hit
TTTTCGCCGAATCCCGGGGCCGAGGAAAGATCGGAAGAGCACACGTCTGA439130.10686508842913892Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGCCGCCGGTGAAATACCACTACTCTCAAGATCGGAAGAGCACACGTCTG428510.10428064364258949Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCGTGGCGCAATGAAGGTGAAGGGCAGATCGGAAGAGCACACGTCTGAAC427770.10410055991923295Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTC422320.10277426763235023No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGT421850.10265989013238051No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)