FastQCFastQC Report
Thu 14 Sept 2023
SRR15228873_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR15228873_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27302727
Total Bases2.3 Gbp
Sequences flagged as poor quality0
Sequence length85
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACAC13761475.040328022911411No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACA12786454.683213511968969No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACG12007014.397732871152395No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCAGATCGGAAGAGCACAC11920604.366084017907808No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACACG4790661.7546452411145597No Hit
GTGGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACG4686051.7163303870708593No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT4646761.7019398831479362No Hit
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCCGAGATCGGAAGAGCAC3925581.4377977701641305No Hit
CTCGCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCACACGTCT3556291.302540218784739Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTGGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACAC3457601.2663936463196515No Hit
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCAGATCGGAAGAGCAC3307371.2113698386245448No Hit
GGGCCCGGTGGGGGGCGGGGCGGACTGTAGATCGGAAGAGCACACGTCTG2723130.9973838876973719Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACG2700030.988923194375419No Hit
CCGTGGCGCAATGAAGGTGAAGGGCCAGATCGGAAGAGCACACGTCTGAA2688700.9847734257460803Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATG2577680.9441108208714829TruSeq Adapter, Index 6 (100% over 49bp)
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACAC2552560.9349102747135845No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCAGATCGGAAGAGCACA2375530.8700705977098917No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGT2364520.8660380334902077No Hit
GGGGCCCGGTGGGGGGCGGGGCGGACTGTAGATCGGAAGAGCACACGTCT2293640.8400772567516791Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCAGATCGGAAGAGCACACG2047970.7500972338770409No Hit
CTCGCTTCTGGCGCCAAGCGTCCGTCCCAGATCGGAAGAGCACACGTCTG1736860.6361489092280049Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGTGGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACA1729360.6334019308767216No Hit
TGCGTGCATTTATCAGATCAAAACCAAAGATCGGAAGAGCACACGTCTGA1668830.611231984262964Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACG1648870.6039213592107484No Hit
CCGTGGCGCAATGAAGGTGAAGGGCCCAGATCGGAAGAGCACACGTCTGA1633670.5983541497521475Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCAGATCGGAAGAGCACAC1438560.526892423602961No Hit
TCTCGCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCACACGTC1349670.4943352361835505No Hit
GCCTCGGTTGGCCCCGGATAGCCGGGTCCCCAGATCGGAAGAGCACACGT1317400.4825159039974285No Hit
TCCCGTGGATCGCCTCAGCTGCGGCGGGCGTCGCGAGATCGGAAGAGCAC1295140.47436287225081947No Hit
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACGT1294090.4739782952816398No Hit
ATGCGTGCATTTATCAGATCAAAACCAAAGATCGGAAGAGCACACGTCTG1234340.45209403441641566Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGCGTGCATTTATCAGATCAAAACCAACCAGATCGGAAGAGCACACGTCT1207290.4421865991627869Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGGGGGGGGATGCGTGCATTTATCAGAAGATCGGAAGAGCACACGTCTGA1158770.4244154805488844Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGGGGGGGGATGCGTGCATTTATCAGATAGATCGGAAGAGCACACGTCTG1140900.4178703467972265Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCGTGGCGCAATGAAGGTGAAGGGCCCCAGATCGGAAGAGCACACGTCTG1019920.3735597546721249Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGCCCGTGGACGGTGTGAGGCCGGTAGCAGATCGGAAGAGCACACGTCTG1004930.36806946060735984Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCCCGCCCCTTGCCTCTCGGCGCCCCAGATCGGAAGAGCACACGTCTGAA962000.3523457565246138Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATGCGTGCATTTATCAGATCAAAACCAACCAGATCGGAAGAGCACACGTC921560.3375340492544939No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCAC917280.33596644027536154No Hit
CGTGGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACAC896290.3282785635295698No Hit
CGGTGGGGGGCGGGGCGGACTGTAGATCGGAAGAGCACACGTCTGAACTC853970.31277828035272814Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCAGATCGGAAGAGCACACGTC850300.31143409227950014No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCAGATCGGAAGAGCACA837370.3066983016018876No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGAGATCGGAAGAGCACACGTC825920.3025045813189283No Hit
CGGGGCCCGGTGGGGGGCGGGGCGGACTGTAGATCGGAAGAGCACACGTC816200.2989444973756651No Hit
TGCGTGCATTTATCAGATCAAAACCAACAGATCGGAAGAGCACACGTCTG808160.2959997365830893Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATGCGTGCATTTATCAGATCAAAACCAAGATCGGAAGAGCACACGTCTGA792170.29014317873815315Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCAGATCGGAAGAGCACACG790670.28959378306789646No Hit
GGGCCCGGTGGGGGGCGGGGCGGACTGTCAGATCGGAAGAGCACACGTCT731180.2678047507855168Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTAGATCGGAAGAGCACA716470.26241701057919964No Hit
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCAGATCGGAAGAGCAC703870.25780208694904355No Hit
TGCGTGCATTTATCAGATCAAAACCAAGATCGGAAGAGCACACGTCTGAA702850.25742849789326905Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGT679940.2490373946895488No Hit
ATGCGTGCATTTATCAGATCAAAACCAACAGATCGGAAGAGCACACGTCT640110.23444910832533325Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGCCCGGTGGGGGGCGGGGCGGACTGTAGATCGGAAGAGCACACGTCTGA610620.223647989448087Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTCGCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGT592320.21694536227095557No Hit
AGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCAGATCGGAAGAGCACA590250.21618719624600136No Hit
GGGGATGCGTGCATTTATCAGATCAAAACCAGATCGGAAGAGCACACGTC588280.21546565659906428No Hit
TCTCGCTTCTGGCGCCAAGCGTCCGTCCCAGATCGGAAGAGCACACGTCT577620.21156128470244018Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCCGAGATCGGAAGAGCA552420.20233143744212806No Hit
CCCGTGGATCGCCTCAGCTGCGGCGGGCGTCGCGAGATCGGAAGAGCACA542390.19865781172701175No Hit
CCAACTTCTTAGAGGGACAAGTGGCGTTAGATCGGAAGAGCACACGTCTG538950.1973978643232231Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCGCAGATCGGAAGAGCA519770.1903729250195411No Hit
CTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCCGAGATCGGAAGAGCACA517010.18936203698626883No Hit
CCAACTTCTTAGAGGGACAAGTGGCGTAGATCGGAAGAGCACACGTCTGA505070.1849888474510257Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCAC480400.17595311999420424No Hit
TCTCGCTTCTGGCGCCAAGCGTCCGTCCAGATCGGAAGAGCACACGTCTG476530.17453567916494203Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACG450010.16482236371480402No Hit
ACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGAGATCGGAAGAGCACACGT438430.1605810291404225No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGT429120.1571711133470294No Hit
GGGGCCCGGTGGGGGGCGGGGCGGACTGTCAGATCGGAAGAGCACACGTC415090.15203243251122864No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTAGATCGGAAGAGCACAC406830.14900709368701523No Hit
AGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACAC398850.1460843087212497No Hit
GGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCAGATCGGAAGAGCACAC393300.14405154474130002No Hit
GGCCCGGTGGGGGGCGGGGCGGACTGTCAGATCGGAAGAGCACACGTCTG387900.14207372032837598Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGGGCGGGCGTCCCGCGCGTGTGGGGGGAAGATCGGAAGAGCACACGTCT386530.14157193894954156Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCT386440.14153897520932615Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCGAGATCGGAAGAGCA375050.13736723075317714No Hit
TGGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACACG374850.1372939779971429No Hit
TCCCGTGGATCGCCTCAGCTGCGGCGGGCGTCAGATCGGAAGAGCACACG374240.13707055709123853No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTAGATCGGAAGAGCACACGT373410.1367665581536965No Hit
AGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTAGATCGGAAGAGCACACG368750.13505976893809912No Hit
CCCCGCCCCTTGCCTCTCGGCGCCCAGATCGGAAGAGCACACGTCTGAAC365260.13378150834530192Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACCGCCCGTCCCCGCCCCTTGCCTCTCGGCAGATCGGAAGAGCACACGTC354960.13000899140953942No Hit
GGGGATGCGTGCATTTATCAGATCAAAACCAAGATCGGAAGAGCACACGT349990.12818866042208898No Hit
GTGCATTTATCAGATCAAAACCAACCAGATCGGAAGAGCACACGTCTGAA349020.12783338455532298Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCAGATCGGAAGAGCACAC343410.125778644748563No Hit
ATGCGTGCATTTATCAGATCAAAACCAGATCGGAAGAGCACACGTCTGAA335770.12298038946805571Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGCGGGCGGGCGTCCCGCGCGTGTGGGGGGAAGATCGGAAGAGCACACGT333330.12208670584443818No Hit
AGCCTCGGTTGGCCCCGGATAGCCGGGTCCCAGATCGGAAGAGCACACGT330110.12090733647228719No Hit
TGGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT328630.12036526607763394No Hit
CTCGCCCGTCACGTTGAACGCACGTTCGTGAGATCGGAAGAGCACACGTC317830.11640961725178588No Hit
GAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCAGATCGGAAGAGCACACG316290.11584557103032236No Hit
AGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCAGATCGGAAGAGCACACG312170.11433656425601736No Hit
GGGAAATGTGGCGTACGGAAGACCCACTCCCCGGCGCAGATCGGAAGAGC307800.11273599153666958No Hit
CCGACGAAGCCGAGCGCACGGGGTCGGCGGCGATAGATCGGAAGAGCACA296100.10845070530866752No Hit
CCCGACGTACGCAGTTTTATCCGGTAAAGCAGATCGGAAGAGCACACGTC292960.10730063703893022No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCCAGATCGGAAGAGCACAC282280.1033889398667027No Hit
GGGGCGGGCGGGCGTCCCGCGCGTGTGGGGGGAAGATCGGAAGAGCACAC273360.10012186694757634No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)