FastQCFastQC Report
Thu 14 Sept 2023
SRR15228874_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR15228874_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25907402
Total Bases2.2 Gbp
Sequences flagged as poor quality0
Sequence length85
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACG17879426.901278638437No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATG15426015.954286732417245TruSeq Adapter, Index 7 (100% over 49bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACAC6393532.467839114087935No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT5872562.266749865540358No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCAGATCGGAAGAGCACAC4441831.7145022878017642No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACA4402271.699232520497424No Hit
CTCGCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCACACGTCT4060141.567173736679579Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCCGTGGATCGCCTCAGCTGCGGCGGGCGTCGCGAGATCGGAAGAGCAC3578141.3811265212930266No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACAC3406191.314755528169131No Hit
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCCGAGATCGGAAGAGCAC3365071.2988836163502615No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGT3127381.2071376357999926No Hit
GTGGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACG2675011.0325273062887588No Hit
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCAGATCGGAAGAGCAC2041560.7880218942833404No Hit
TGCGTGCATTTATCAGATCAAAACCAAAGATCGGAAGAGCACACGTCTGA1799480.6945814173107747Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACACG1789740.690821873995702No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACG1769270.6829206571928749No Hit
TGCGTGCATTTATCAGATCAAAACCAACCAGATCGGAAGAGCACACGTCT1733260.6690211546491617Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCTCGCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCACACGTC1635510.6312906249727395No Hit
GTGGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACAC1453600.5610751707176196No Hit
CCGTGGCGCAATGAAGGTGAAGGGCCAGATCGGAAGAGCACACGTCTGAA1429930.5519387856798609Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCCGAGATCGGAAGAGCACA1282800.4951480661781525No Hit
CTCGCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGT1224800.4727606419200196No Hit
CGGGGGGGGATGCGTGCATTTATCAGAAGATCGGAAGAGCACACGTCTGA1216850.46969202083636175Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGCGTGCATTTATCAGATCAAAACCAACAGATCGGAAGAGCACACGTCTG1172990.4527624962163323Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTCGCCCGTCACGTTGAACGCACGTTCGTGAGATCGGAAGAGCACACGTC1155580.4460424090381583No Hit
GGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCAGATCGGAAGAGCACACG1143140.44124069252486225No Hit
GCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACG1113630.42985012545835355No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCAGATCGGAAGAGCACA980630.3785134456940144No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGT946470.365328024786121No Hit
GGGGCCCGGTGGGGGGCGGGGCGGACTGTAGATCGGAAGAGCACACGTCT943490.36417777436734106Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTCGCCCGTCACGTTGAACGCACGTTCGTAGATCGGAAGAGCACACGTCT916200.3536441052638161Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCAGATCGGAAGAGCAC870770.3361085762285234No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGAGATCGGAAGAGCACACGTC844400.3259300179925413No Hit
CGTGGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACAC822420.31744595617885574No Hit
GCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCCGAGATCGGAAGAGCA819250.31622236764612677No Hit
GGAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCAGATCGGAAGAGCACAC725340.27997403985162234No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCAGATCGGAAGAGCACA723170.2791364413923094No Hit
CTCGCCCGTCACGTTGAACGCACGTTCGAGATCGGAAGAGCACACGTCTG694640.2681241445977485Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGTGGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACA683230.263719997860071No Hit
ATGCGTGCATTTATCAGATCAAAACCAAAGATCGGAAGAGCACACGTCTG668390.25799190517057635Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCCGTGGATCGCCTCAGCTGCGGCGGGCGTCGCGAGATCGGAAGAGCACA652500.2518585229039948No Hit
TCCCGTGGATCGCCTCAGCTGCGGCGGGCGTCAGATCGGAAGAGCACACG626870.24196559732234052No Hit
ACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGAGATCGGAAGAGCACACGT610570.23567395912565836No Hit
CGGGGGGGGATGCGTGCATTTATCAGATAGATCGGAAGAGCACACGTCTG599390.23135859010486656Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATGCGTGCATTTATCAGATCAAAACCAACCAGATCGGAAGAGCACACGTC581960.22463078312522422No Hit
GGAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCAC545340.2104958266367272No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGT541040.20883606932103807No Hit
CGGGGCCCGGTGGGGGGCGGGGCGGACTGTAGATCGGAAGAGCACACGTC524720.20253671132288756No Hit
ACCGCCCGTCCCCGCCCCTTGCCTCTCGGCAGATCGGAAGAGCACACGTC517100.19959546696345698No Hit
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACGT505920.1952800979426652No Hit
TCTCGCTTCTGGCGCCAAGCGTCCGTCCCAGATCGGAAGAGCACACGTCT503680.19441548017821317Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACG492800.19021590817944617No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCAGATCGGAAGAGCACACG486750.18788066823527885No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCT473240.18266594234342756Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCGTGGCGCAATGAAGGTGAAGGGCCCAGATCGGAAGAGCACACGTCTGA461880.17828109510942086Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGGCCCGGTGGGGGGCGGGGCGGACTGTAGATCGGAAGAGCACACGTCTG451330.17420889983488116Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGGTGGGGGGCGGGGCGGACTGTAGATCGGAAGAGCACACGTCTGAACTC437110.16872012099090444Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CCCCGCCCCTTGCCTCTCGGCGCCCCAGATCGGAAGAGCACACGTCTGAA430380.16612240779681423Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATGCGTGCATTTATCAGATCAAAACCAACAGATCGGAAGAGCACACGTCT414920.16015500126180154Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTCCCGGGGCTACGCCTGTCTGAGAGATCGGAAGAGCACACGTCTGAACT404290.1560519267813886Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CCGTGGCGCAATGAAGGTGAAGGGCCCCAGATCGGAAGAGCACACGTCTG401570.15500203378169683Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGCGTGCATTTATCAGATCAAAACCAACCCGGAGATCGGAAGAGCACACG394230.15216886664282278No Hit
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTAGATCGGAAGAGCACA386890.1493356995039487No Hit
GTGCATTTATCAGATCAAAACCAACCCGGTAGATCGGAAGAGCACACGTC372220.14367322512693476No Hit
CGCGTCTCTCGGGGTCGGGGGTGCGTGGCGGGGGAGATCGGAAGAGCACA356070.13743948544126502No Hit
CCTCGGTTGGCCCCGGATAGCCGTGTCCCCGAGATCGGAAGAGCACACGT349100.1347491346295549No Hit
CTCGCTTCTGGCGCCAAGCGTCCGTCCCAGATCGGAAGAGCACACGTCTG345570.13338658967039613Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCAGATCGGAAGAGCACA343480.1325798704169565No Hit
CCAACTTCTTAGAGGGACAAGTGGCGTAGATCGGAAGAGCACACGTCTGA342720.13228651796116028Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGGAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCAGATCGGAAGAGCACA336750.12998215722286627No Hit
CTAAACCATTCGTAGACGACCTGCTTCAGATCGGAAGAGCACACGTCTGA331760.12805606675652Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCTCGCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACG321430.12406878929813185No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCAC314170.12126650136513109No Hit
GGGGATGCGTGCATTTATCAGATCAAAACCAGATCGGAAGAGCACACGTC310990.12003905293166794No Hit
TCTCGCCCGTCACGTTGAACGCACGTTCGAGATCGGAAGAGCACACGTCT310960.12002747322946547Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTC309680.11953340593549287No Hit
CCAACTTCTTAGAGGGACAAGTGGCGTTAGATCGGAAGAGCACACGTCTG308870.11922075397602584Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTAGATCGGAAGAGCACAC292890.11305263260283684No Hit
ATGCGTGCATTTATCAGATCAAAACCAAGATCGGAAGAGCACACGTCTGA278650.10755613395739179Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCGAGATCGGAAGAGCA271170.10466892820823949No Hit
TGGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT270260.10431767724143086No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGTC263140.10156942791870834No Hit
CCGACGTACGCAGTTTTATCCGGTAAAGCAGATCGGAAGAGCACACGTCT262080.10116027844088728Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)