FastQCFastQC Report
Thu 14 Sept 2023
SRR15283612_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR15283612_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47775208
Total Bases2.4 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC1668640.349269018357806Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TAACGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC1338530.28017251123218556Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ACACGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC1212320.2537550438294272Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AACCGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC1201710.2515342267060355Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AATCGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC1125200.23551964441473494Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CAACGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC1111460.2326436757742635Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ATACGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC1100180.23028261854977167Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GAACGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC1022910.21410895793483514Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TTACGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC1013790.21220001805120348Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TACCGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC974240.20392166581462084Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TATCGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC865320.1811232302745809Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCACGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC831770.17410075954038756Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CACCGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC790780.1655209957432315Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CTACGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC786990.16472769726088896Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GTACGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC784440.16419394762237352Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGACGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC771760.16153985138065752Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GACCGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC767710.16069213136654475Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CCACGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC763000.15970626438716917Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AAGCGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC755660.15816990268257963Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CATCGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC743840.15569581612287275Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TTTCGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC741890.15528765463459626Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GCACGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC739000.1546827383776121Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ACCCGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC725650.15188840203479595Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ACTCGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC713920.14943315369762492Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGACGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC708350.1482672770362402Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GATCGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC651750.13642012819703475Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ACGCGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC645580.1351286633854111Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ATTCGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC639980.13395650731651446Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TAGCGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC631270.1321333860022127Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ATCCGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC612760.1282589915673418Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ATGCGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC591190.12374409756625235Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGACGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC589350.1233589605721863Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TTCCGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC580060.12141443737932024Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGCCGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC548130.114731054650772Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGTCGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC538540.11272373738278649Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CAGCGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC520660.10898121050566645Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CCTCGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC520600.10896865169064256Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGACGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC513220.10742391744270376Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCTCGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC510260.10680434923485839Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCCCGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC494140.10343021426510587Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CTTCGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC490060.10257621484348117Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GTTCGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC482390.1009707796562602Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TTGCGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC478950.1002507409282237Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)