FastQCFastQC Report
Thu 14 Sept 2023
SRR15283618_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR15283618_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40677859
Total Bases2 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AACTAGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC904510.22235929378682392Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ACCTAGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC765060.18807774519302994Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AAATAGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC764410.18791795310564402Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ACATAGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC746930.18362077512486585Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CACTAGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC674350.1657781448133738Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TACTAGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC656760.16145392509473028Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AAGTAGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC653320.1606082562014879Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CCATAGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC644440.15842525045381567Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CAATAGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC642790.15801962438583605Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ACTTAGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC633370.15570386828864322Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CCCTAGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC632120.1553965758129011Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TAATAGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC613170.1507380218806501Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ACGTAGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC607300.149294976414565Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AATTAGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC582420.14317862697739328Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ATCTAGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC563600.1385520314626195Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CCTTAGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC560680.13783419623928586Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCCTAGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC556440.1367918601615685Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GACTAGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC552430.13580606589938768Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ATATAGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC537730.13219230638466Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CATTAGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC536510.13189238892833569Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CTATAGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC532520.13091151134576676Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GAATAGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC497500.12230240534537473Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCATAGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC496440.12204182132594539Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ATGTAGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC481920.11847231192772462Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TAGTAGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC467350.11489051083047415Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGATAGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC461980.11357038235468588Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CCGTAGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC460150.11312050617019936Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GTATAGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC459410.11293858902456003Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TATTAGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC456300.11217404534491357Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GCCTAGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC453650.1115225852963402Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TTATAGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC452330.11119808444195649Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CTCTAGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC447320.10996645619918197Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GATTAGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC445510.10952149669430733Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TTCTAGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC440280.10823578497580219Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CTTTAGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC429630.10561765308247911Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCTTAGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC429300.10553652786888318Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CTGTAGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC427460.10508419334459072Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGCTAGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC414250.10183672646094771Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGTTAGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC412430.10138930861626715Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ATTTAGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC409020.10055101474244256Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CAGTAGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC408500.10042318107253384Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GAGTAGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC407030.10006180512106107Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)