FastQCFastQC Report
Sat 16 Sept 2023
SRR15283621_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR15283621_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49915799
Total Bases4.1 Gbp
Sequences flagged as poor quality0
Sequence length84
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAGATCGG16590693.3237352366131616No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCAAAAAAAAAAAAAGATCG12726162.549525451851427No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAAAAAGATCGG7722731.5471514339578136No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAAAAAAAAAAAAAGATCGGA7424251.4873547351210386No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACAAAAAAAAAAAAAGAT6010901.2042079102049434No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAGATCGGA4775480.9567071139139735No Hit
TTTCGCCGAATCCCGGGGCCGAGGAAGCCAAAAAAAAAAAAAGATCGGAA4610590.9236734846215724No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAAAAAAAAAAAAAGATCGGA4540830.909697949540986No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAAAAAGATCG4230050.8474371010268713No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAGATCGGA4113890.8241659118789223No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAAAAAAGATCGGA3919010.7851241647959998No Hit
TTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAAAAAAAAAAAAAGATCGG3522430.70567436975215No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAGATCG3095910.6202264737863857No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAAAAAAGATCGG2959100.5928183179037162No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCAAAAAAAAAAAAAGATC2888980.5787706613691589No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCAAAAAAAAAAAAGATCGG2307470.46227247609519384No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAATAAAAAAAAAAAAAGATC2256630.4520873240955233No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCAAAAAAAAAAAAAAGATC2174870.4357077405492398No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCAAAAAAAAAAAAAGATCGGAA1961320.3929256947284365No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGAAAAAAAAAAAAAGATCGGAA1872580.37514775632460573No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAAAAGATCGGA1828530.3663228950817756No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACAAAAAAAAAAAAAAGA1584800.317494667369744No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAAAAAAGATCG1568490.3142271648301172No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGAAAAAAAAAAAAAGATCGGAA1552460.31101575675469No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGAAAAAAAAAAAAAG1396290.27972906934736236No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAAAAAAAAAAAAAAGATCGG1384860.2774392131837858No Hit
GCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGAAAAAAAAAAAAAGA1301020.26064292790344795No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCCAAAAAAAAAAAAAGATCGG1276360.2557026083064402No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAAAAAAAAAAAAAGA1273440.2551176231797872No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAGATCGGAA1213320.24307334036664424No Hit
TTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAAAAAAAAAAAAAAGATCG1171480.23469122471624668No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAAAAAAAAAAAAGATCGGAA1163140.2330204110325871No Hit
TTTCGCCGAATCCCGGGGCCGAGGAAGCCAAAAAAAAAAAAGATCGGAAG1116200.2236165747842682No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAATAAAAAAGATCGG1092470.218862568943352No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAAAAGATCGG1055660.21148815027482581No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCAAAATAAAAAAAAGATCG1041580.20866740007507442No Hit
GTCGCGGCGTGGGAAATGTGGCGTACGAAAAAAAAAAAAAGATCGGAAGA1003860.20111067439789956No Hit
ACGGTGGCCATGGAAGTCGGAATCCGCTAAAAAAAAAAAAAGATCGGAAG994580.19925154358442707No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAGATCGGAA993760.1990872669392711No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAAAAAAAGATCGG981200.19657102954517466No Hit
TTGGATAACTGTGGTAATTCTAGAGCTAATACATGCAAAAAAAAAAAAAG946190.1895572181465031No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCAAAAATAAAAAAAGATCG935140.1873434901843402No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAGATCGG911680.1826435754339022No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACAAAAAAAAAAAAGATC906900.18168596279506613No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAGCCAAAAAAAAAAAAAGATCGGA830930.16646633263348146No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAAAAAAGATC813500.16297445223705626No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAATAAAAAGATCGG808990.16207093068869838No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTGAAAAAAAAAAAAAGATCG807740.1618205089735216No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAAAAAAAAAAAAAAGATCGG800790.16042816423713863No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACGAAAAAAAAAAAAAGA798910.16005152997751274No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAATAAAAAAAGATCGG797230.15971496319231512No Hit
TTTCGCCGAATCCCGGGGCCGAGGAAGCCAGGAAAAAAAAAAAAAGATCG796240.1595166291938951No Hit
TTTCGCCGAATCCCGGGGCCGAGGAAGCCAAAAAAAAAAAAAAGATCGGA782380.15673995321601483No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCAAAAAAAAAAAGATCGGA773470.15495494723023467No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAATAAAAAAAAAAAAAAGAT763660.1529896376095272No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAAAAAAAGATCG709770.14219345662482533No Hit
TGGTGACTCTAGATAACCTCGGGCCGATCGCAAAAAAAAAAAAAGATCGG699860.14020811326690374No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAAAAAAAAAAAAAGATCG679100.13604910942124757No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAAAAAAAAAAAAGATC667270.13367911830881443No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCGAAAAAAAAAAAAAGATC658950.1320123113725977No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCAAAAAATAAAAAAGATCG645680.12935383444428086No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGGAAAAAAAAAAAAAGATCGG637930.12780121981018475No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAAAAAGATCGGA611660.1225383570440293No Hit
GTCGCGGCGTGGGAAATGTGGCGTACGGAAAAAAAAAAAAAGATCGGAAG608740.12195337191737629No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAATAAAAAAGATCGG608370.12187924708968398No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCAAAAAAAAAAAAAGA601090.12042079102049434No Hit
GTCGCGGCGTGGGAAATGTGGCGTACAAAAAAAAAAAAAGATCGGAAGAG599340.12007020061924684No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACAATAAAAAAAAAAGAT594060.11901241929434005No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAAAAAGATCGG590380.11827517776485957No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAAAAAAAAAAAAGATCGGAA569540.1141001469294321No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCGAAAAAAAAAAAAAGATCGG567630.11371750254864196No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCAAAAAAAAAAAAAGATCGGA566220.11343502685392255No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAAAAAATAAAAAAGATCGGA537520.1076853442734634No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCAAAAAAAAAAAAGATCG527920.10576210550090562No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGAAAAAAAAAAAAAGATC527420.10566193681483492No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAAAAAAATAAAAAGATCGGA520710.1043176730477659No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTTGAAAAAAAAAAAAAGATCGGA519650.10410531543329597No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAATAAAAAAAGATCGG517200.10361448887154948No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCGCAAAAAAAAAAAAAGAT507660.1017032703413202No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGAAAAAAAAAAAAAAGATCGGA507160.10160310165524948No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)