FastQCFastQC Report
Thu 14 Sept 2023
SRR15283622_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR15283622_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36139419
Total Bases1.8 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAATAGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC1562460.43234231297409625Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AACTAGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC1225590.3391283075137428Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ATATAGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC1199390.3318786060174349Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TAATAGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC1155070.3196149888297872Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ACATAGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC1099970.30436847919442206Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AATTAGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC1097300.30362967373659216Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CAATAGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC967780.26779069137774464Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TTATAGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC917020.2537450864940579Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ATTTAGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC910630.2519769341062179Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ACCTAGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC907860.25121045803198994Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AAGTAGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC902130.24962493171237754Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GAATAGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC873410.2416779306828369Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TACTAGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC868410.24029439986293083Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ATCTAGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC858080.2374360251890048Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ACTTAGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC828300.22919571562564411Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CTATAGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC796160.22030237951528772Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TATTAGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC787670.21795314418308714Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ATGTAGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC777550.2151528778035972Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CACTAGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC762080.21087223344680778Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGATAGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC751360.2079059433689291Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCATAGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC746500.20656115141198036Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TTTTAGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC734020.20310785848549473Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CCATAGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC730840.20222793288403448Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CATTAGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC714500.19770655416458133Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GACTAGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC699590.19358086525962137Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GTATAGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC692230.1915443078927196Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ACGTAGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC678270.18768148984354174Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TAGTAGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC638190.17659110679117448Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TTCTAGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC636630.17615944517536378Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCCTAGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC624870.17290538068694464Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GATTAGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC621330.17192584086645113Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGATAGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC618010.17100717640203347Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CTTTAGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC588960.16296886233837904Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGCTAGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC575570.15926376680267051Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GCATAGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC573020.15855816608451842Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CCCTAGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC560520.15509933903475315Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGTTAGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC560420.15507166841835504Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CAGTAGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC556650.15402848618014583Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CCTTAGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC556160.15389290015979504Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TTGTAGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC555030.15358022219449627Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCTTAGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC552170.15278884256550998Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CTCTAGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC543920.15050601671266492Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GTTTAGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC535950.1483006685857346Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GCCTAGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC533650.14766424440857778Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GAGTAGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC517420.1431733033671626Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGATAGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC502760.13911679100319793Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CTGTAGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC497800.13774432842985107Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GTCTAGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC491930.13612006324728132Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGGTAGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC454220.12568547380354952Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGCTAGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC451910.1250462825647529Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CCGTAGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC449510.12438218777119799Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGTTAGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC440990.12202465125407799Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCGTAGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC440380.12185586049404944Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGATAGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC434390.12019839057180194Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GCTTAGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC426860.11811479315702335Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GTGTAGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC423360.11714632158308909Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGTTAGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC385860.10676984043379335Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGCTAGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC370110.10241171835108916Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)