FastQCFastQC Report
Thu 14 Sept 2023
SRR15283623_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR15283623_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42363116
Total Bases3.5 Gbp
Sequences flagged as poor quality0
Sequence length84
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGGTGACTCTAGATAACCTCGGGCCGATCGCAAAAAAAAAAAAAGATCG12666632.9900137657484875No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAGATCGG12130992.8635735860412157No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAAAAAAAAAAAAAGATCGGA7039981.6618182666260906No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACAAAAAAAAAAAAAGAT6900221.6288273034495384No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCAAAAAAAAAAAAAGATC5648541.3333627299748205No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAAAAAGATCGG5391851.2727699256117042No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAAAAAGATCG4510991.0648390453620078No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAAAAAAAAAAAAAGATCGGA4154380.980659685184631No Hit
TTGGATAACTGTGGTAATTCTAGAGCTAATACATGCAAAAAAAAAAAAAG3451530.8147488489751321No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAATAAAAAAAAAAAAAGATC3428370.8092818290325953No Hit
TTTCGCCGAATCCCGGGGCCGAGGAAGCCAAAAAAAAAAAAAGATCGGAA3177490.7500605007431465No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAGATCGGA3058880.7220620881617867No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAAAAAAGATCGG3021390.7132124086434057No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAAAAAAAAAAAAAGA2998440.7077949601252184No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAGATCGGA2976370.7025852394804952No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAAAAAAGATCGGA2825970.667082657470239No Hit
TTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAAAAAAAAAAAAAGATCGG2541560.5999464250929983No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAGATCG2225240.5252776967586614No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCAAAAAAAAAAAAGATCGG2199100.5191072346991662No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCAAAAAAAAAAAAAAGATC2177140.513923480038626No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACAAAAAAAAAAAAAAGA1813620.4281129839457513No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCAAAAAAAAAAAAAGATCGGAA1784160.42115882127273174No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGAAAAAAAAAAAAAGATCGGAA1734890.40952842090274943No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGAAAAAAAAAAAAAGATCGGAA1346000.3177292246396606No Hit
CGTTTTTTCACTGACCCGGTGAGGCGGGGAAAAAAAAAAAAAGATCGGAA1306520.30840979686196834No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGAAAAAAAAAAAAAG1284120.3031221782646961No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAAAAAAAAAAAAAAGATCGG1253920.29599333533444516No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAAAAGATCGGA1224010.28893294818067683No Hit
GCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGAAAAAAAAAAAAAGA1216660.28719794832844686No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAATAAAAAAAAAAAAAAGAT1193060.2816270644491779No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAAAAAAAAAAAAGATCGGAA1113590.26286782114894475No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAAAAAAGATCG1109040.26179377362137385No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAAAAGATCGG1094880.25845124329381247No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACAAAAAAAAAAAAGATC1025700.24212099978670124No Hit
GTCGCGGCGTGGGAAATGTGGCGTACGAAAAAAAAAAAAAGATCGGAAGA1011430.23875250347495683No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCCAAAAAAAAAAAAAGATCGG1010820.23860851028994187No Hit
ACGGTGGCCATGGAAGTCGGAATCCGCTAAAAAAAAAAAAAGATCGGAAG997320.23542177586747867No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCAAAAAAAAAAAAGATCG992740.23434064670785787No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCAAAAAAAAAAAAAAGAT979620.23124361295802698No Hit
GGGCCGCCGGTGAAATACCACTACTCTCAAAAAAAAAAAAAGATCGGAAG919730.21710631484237372No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAAAAAAGATC894990.2112663289452079No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACGAAAAAAAAAAAAAGA866110.2044490778251534No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCAAAATAAAAAAAAGATCG848410.20027091491570168No Hit
TTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAAAAAAAAAAAAAAGATCG844530.1993550238372456No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAAAAAGATCGGA833930.19685284717960785No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCAAAAATAAAAAAAGATCG785080.1853215896583245No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAAAAAAAAAAAAAAG754810.17817622291995708No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAGATCGGAA754210.1780345902789587No Hit
TTTCGCCGAATCCCGGGGCCGAGGAAGCCAAAAAAAAAAAAGATCGGAAG750990.1772744951056008No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAAAAAAAAAAAAAAGATCGG726770.17155725749730025No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAAAAAAAGATCG724280.17096948203715706No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCAAAAAAAAAAAGATCGGA719120.16975144132457112No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAGATCGGAA712620.16821708771375551No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAAAAAAAGATCGG703180.16598873416204793No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAAAAAAAAAAAAGATC697520.16465266624863006No Hit
TTGGATAACTGTGGTAATTCTAGAGCTAATACATGCAAAAAAAAAAAAGA647760.1529065992218325No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCGAAAAAAAAAAAAAGATC631330.149028225402494No Hit
TTGGATAACTGTGGTAATTCTAGAGCTAATACATGCAAAAAAAAAAAAAA631260.14901170159437752No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAGCCAAAAAAAAAAAAAGATCGGA617810.14583676989199756No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAATAAAAAAGATCGG610640.14414425983206713No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAATAAAAAAAAAAAAGATCG608590.14366034830865604No Hit
GTCGCGGCGTGGGAAATGTGGCGTACAAAAAAAAAAAAAGATCGGAAGAG606520.1431717156972117No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCAAAAAAAAAAAAAGA599380.14148628726933118No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGGAAAAAAAAAAAAAGATCGG595310.14052554585455895No Hit
GTCGCGGCGTGGGAAATGTGGCGTACGGAAAAAAAAAAAAAGATCGGAAG585950.1383160766549845No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAGATCGG576430.13606883875114378No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACAATAAAAAAAAAAGAT565050.1333825396602082No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCAAAAAATAAAAAAGATCG549310.1296670433780178No Hit
TTTCGCCGAATCCCGGGGCCGAGGAAGCCAAAAAAAAAAAAAAGATCGGA548760.12953721345710265No Hit
TTTCGCCGAATCCCGGGGCCGAGGAAGCCAGGAAAAAAAAAAAAAGATCG543840.12837582580091605No Hit
TGGTGACTCTAGATAACCTCGGGCCGATCGCAAAAAAAAAAAAAGATCGG536830.12672108444525185No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCGCAAAAAAAAAAAAAGAT530600.12525046552288555No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTGAAAAAAAAAAAAAGATCG528030.1246438057106092No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAAAAAAAAAAAAGATCGGAA525700.12409379895473223No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAAAAAAAAAAAAAGATCG521750.12316138406815967No Hit
CGCGGCGTGGGAAATGTGGCGTACGAAAAAAAAAAAAAGATCGGAAGAGC483880.11422200387714634No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAAAAAGATCGG481790.1137286501776687No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAATAAAAAGATCGG481360.11362714678495323No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGTAAAAAAAAAAAAAGATCG479320.11314559580555877No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGAAAAAAAAAAAAAAGATCGGA478400.11292842575602795No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCACAAAAAAAAAAAAAGATCG478160.11287177269962861No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTTGAAAAAAAAAAAAAGATCGGA468720.11064341914792104No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAAAAAAAAAAAAGAT450990.10645817460641942No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAAAAAAAAAAAAAGATCGG447240.10557297060017966No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCAAAAAAAAAAAAAGATCGGA446250.10533927674253234No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCAAAATAAAAAAAAGATC443850.1047727461785389No Hit
CGCCTGGATACCGCAGCTAGGAATAATGGAAAAAAAAAAAAAGATCGGAA439100.1036514877706352No Hit
ACGGTGGCCATGGAAGTCGGAATCCGCAAAAAAAAAAAAAGATCGGAAGA431110.10176541310134032No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAATAAAAAAAGATCGG428270.10109501860061473No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)