FastQCFastQC Report
Sat 30 Sept 2023
SRR15283625_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR15283625_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45179639
Total Bases2.3 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AACTAGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC2408190.5330255073529915Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AAATAGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC2372310.5250838768322164Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ACCTAGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC2262830.5008517221662617Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ACATAGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC2100650.46495502099961444Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TAATAGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC1941850.4298064444472432Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ATATAGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC1931280.4274668949878063Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CAATAGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC1912250.42325482060624703Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AAGTAGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC1880570.41624281238723493Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CCATAGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC1862610.41226757035398176Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AATTAGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC1820810.4030156150650075Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ATCTAGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC1797820.3979270396560717Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ACTTAGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC1778780.39371275188807947Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CACTAGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC1778580.3936684841594241Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CTATAGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC1760330.3896290539196207Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCCTAGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC1732760.3835267475244767Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TACTAGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC1699700.37620929197774244Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CCCTAGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC1697880.3758064556469785Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ATGTAGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC1653870.36606534195636226Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CCTTAGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC1607870.35588376436562497Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ATTTAGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC1575460.3487101789370207Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCATAGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC1559340.34514220000739715Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TTATAGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC1549080.3428712655273762Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CATTAGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC1519780.33638604327936306Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GAATAGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC1517280.3358326966711708Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ACGTAGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC1511110.3344670372421524Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GCCTAGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC1482400.32811240479367265Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CTTTAGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC1411340.3123840808024163Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GACTAGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC1409910.3120675665425304Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGATAGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC1392370.3081852867394536Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TATTAGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC1371370.3035371752306388Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GTATAGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC1368640.3029329207344928Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TAGTAGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC1353560.2995951339938772Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TTCTAGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC1345760.2978686925763174Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CTCTAGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC1322010.2926118997984911Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCTTAGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC1315710.2912174663458466Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CTGTAGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC1262170.2793669953848016Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CAGTAGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC1238620.2741544703356306Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GATTAGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC1236400.2736630985475559Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CCGTAGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC1224740.2710822899669473Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TTTTAGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC1215610.2690614681538292Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGTTAGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC1186930.26271347586464777Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GTTTAGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC1177510.2606284658449794Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GAGTAGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC1163620.2575540720898633Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TTGTAGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC1162560.2573194531279898Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGCTAGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC1147390.2539617459094793Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGATAGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC1124000.24878463504323264Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GTGTAGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC1116230.24706483378497116Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GCATAGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC1105250.24463453548179082Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGGTAGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC1064920.23570794799843356Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GTCTAGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC1055260.2335698167043787Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGATAGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC1044440.2311749325841227Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCGTAGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC1009770.22350112182171264Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGTTAGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC1008750.2232753564055702Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGTTAGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC961180.21274627714488822Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GCTTAGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC959760.212431976271435Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGATAGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC928710.20555941139768735Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGGTAGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC896500.1984300936977385Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGCTAGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC882340.19529593850893762Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGCTAGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC855920.18944817155356197Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GCGTAGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC808090.17886154424562797Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGGTAGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC807340.1786955402631703Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGTTAGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC765690.16947678577068753Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGCTAGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC761780.16861135167547486Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGGTAGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC659600.1459949691054415Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AAATAGTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCC476850.1055453320465885Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)