FastQCFastQC Report
Sat 30 Sept 2023
SRR15283627_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR15283627_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47661888
Total Bases2.4 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAGCTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC2907560.6100387798318019Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AACGCTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC2677310.561729740962003Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CCAGCTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC2604260.54640302960722Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TAAGCTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC2554180.5358956825210114Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ACCGCTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC2462910.5167462103053913Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ACAGCTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC2396400.5027916644846298Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CAAGCTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC2322050.48719219851299217Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CCCGCTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC2305820.48378696202718613Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CACGCTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC2235920.4691211560901658Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ATAGCTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC2193150.4601475291956542Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TACGCTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC2189070.4592914993212187Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CTAGCTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC2096080.4397811517663757Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GAAGCTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC2016560.42309696166463234Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AATGCTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC1963640.4119937506462186Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCAGCTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC1955650.4103173588087824Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGAGCTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC1934420.40586306610430545Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TTAGCTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC1919690.4027725464841007Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GACGCTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC1827390.3834069686874343Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AAGGCTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC1816890.38120395062822526Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCCGCTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC1732530.36350427410680836Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CCTGCTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC1730290.36303429692084377Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGAGCTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC1730050.36298394222234753Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ACTGCTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC1716930.3602312187045549Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TATGCTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC1701320.35695606518986406Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CATGCTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC1617980.3394703961370561Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ATCGCTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC1594310.33450416399786764Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GTAGCTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC1553480.3259375709162004Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGCGCTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC1548470.32488641658509204Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GCCGCTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC1544640.32408283952159006Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GCAGCTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC1540480.3232100247476558Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGAGCTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC1473870.309234497802521Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TAGGCTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC1453450.3049501522054686Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGTGCTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC1450980.3044319184334452Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GATGCTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC1438920.3019015948340108Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ACGGCTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC1437910.30168968547783925Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CTCGCTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC1425900.2991698524405915Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ATGGCTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC1420810.29810191320998447Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGAGCTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC1390840.29181387023527056Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TTCGCTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC1343620.2819065833061418Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGGGCTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC1338020.28073164034123027Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GAGGCTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC1335330.2801672480955853Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ATTGCTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC1298840.2725112358117245Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CTTGCTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC1297370.27220281328343515Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCTGCTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC1292380.27115585517720153Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGTGCTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC1280020.26856258820464685Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGCGCTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC1218930.2557452193249248Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TTTGCTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC1194450.25060904007831164Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CCGGCTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC1190760.24983483658893246Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGTGCTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC1179390.2474492827476746Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGGGCTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC1166450.2447343252537541Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGCGCTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC1158400.24304534474169381Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CAGGCTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC1146830.24061782865168915Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TTGGCTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC1062090.2228384238576533Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGCGCTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC1059940.2223873296836248Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGTGCTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC1054320.22120819049383858Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GCTGCTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC1043960.2190345460087523Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GTCGCTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC1037690.21771902951053893Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CTGGCTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC1036720.21751551260411672Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGGGCTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC1029870.21607830558453747Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GTTGCTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC1008990.21169744681536745Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GTGGCTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC1007590.21140371107413958Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCGGCTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC969540.2034203932500534Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGGGCTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC882180.1850912829974339Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GCGGCTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC846140.17752968577325348Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGAGCTTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCC730410.15324823053589484Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AAAGCTTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCC682950.14329058890826984Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGCGCTTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCC629300.1320342156819302Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGAGCTTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCC622450.13059700866235094Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CAAGCTTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCC576860.1210317140605089Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATAGCTTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCC562140.11794329255274151Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TAAGCTTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCC544680.11427998823714243Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AACGCTTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCC538480.11297915852599041Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ACAGCTTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCC519040.10890042794779761Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATCGCTTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCC515740.10820805084347476Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCGCTTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCC512450.10751777185158927Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGAGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCA501220.10516159158445422Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AATGCTTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCC486820.1021403096746818Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CCAGCTTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCC485550.10187384939513935Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CTAGCTTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCC484440.1016409589145944Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGAGCTTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCC483340.10141016654648678Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TACGCTTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCC482570.10124861188881146Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AAAGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCA482430.10121923831468867Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ACCGCTTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCC481220.1009653667097703Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)