FastQCFastQC Report
Sat 30 Sept 2023
SRR15283629_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR15283629_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33405741
Total Bases1.7 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACGGTCGGGGAGGTAGTGACGAAAAATAACAAGTCACTGTAGGCACCA719250.215307303017167No Hit
AAACGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC602290.18029535701662777Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ATACGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC502850.15052801852232525Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AACCGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC494760.14810627909735635Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AAACGGTCGGGGAGGTAGTGACGAAAAATAACAACTCCCTGTAGGCACCA482320.14438236828813347No Hit
ACACGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC481920.1442626283907308Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TAACGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC463350.1387037036538121Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ACCCGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC430780.12895388250780007Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CAACGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC425620.1274092378313057Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ATACGGTCGGGGAGGTAGTGACGAAAAATAACAAGTCACTGTAGGCACCA416680.12473305112435615No Hit
CCACGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC389960.1167344259778581Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AATCGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC375320.1123519457329206Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AAGCGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC372150.11140300704600445Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CACCGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC371090.11108569631788741Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGCCGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC370630.11094799543587433Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TACCGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC368090.11018764708736742Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ATCCGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC364250.10903814407230182Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GAACGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC363920.10893935865694464Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ATTCGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC357550.10703250079080717Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CAACGGTCGGGGAGGTAGTGACGAAAAATAACAAGTCACTGTAGGCACCA357080.10689180641135906No Hit
CCCCGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC355960.10655653469863159Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CTACGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC353610.10585306280139094Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TTACGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC353310.10576325787833894Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ACACGGTCGGGGAGGTAGTGACGAAAAATAACAAGTCACTGTAGGCACCA350260.10485024116064362No Hit
TCACGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC342590.10255422862794751Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TAACGGTCGGGGAGGTAGTGACGAAAAATAACAAGTCACTGTAGGCACCA336510.100734182187427No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)