FastQCFastQC Report
Sat 30 Sept 2023
SRR15283630_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR15283630_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48305255
Total Bases2.4 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC6029871.2482844775376924Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CACAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC5094501.054647160024308Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ACCAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC5033011.0419176961181553Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ACAAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC4897521.0138689879600056Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AAAAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC4875981.0094098457817893Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TACAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC4871571.0084969016310958Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CAAAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC4707770.9745875474624863Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CCAAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC4289190.8879344493678794Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GACAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC4124470.8538346397301909Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TAAAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC4059820.8404510026911151Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CCCAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC3856290.7983168704936969Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GAAAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC3744870.7752510570537304Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AATAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC3541260.7331003635111749Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CATAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC3508820.7263847380580022Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ACGAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC3447910.7137753439040949Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CTAAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC3441860.7125228921780871Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ACTAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC3432450.7105748639563129Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCCAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC3418750.7077387336015513Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCAAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC3361310.6958476877929741Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ATAAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC3356040.6947567091820549Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AAGAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC3338460.6911173535881345Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GCCAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC3326800.6887035375343739Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGAAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC3326700.6886828358529522Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ATCAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC3301280.6834204684355771Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGCAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC3280450.6791083081954541Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CCTAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC3210060.6645363946427775Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGAAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC3200350.6625262613767384Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GCAAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC3059570.6333824342713852Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGAAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC2913650.6031745407409608Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CTCAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC2899760.6002990771914981Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GTAAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC2888310.5979287346687229Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GATAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC2833530.5865883535859608Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TTAAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC2824980.5848183598244125Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TATAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC2815190.5827916652132361Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CAGAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC2708620.5607298833222183Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGCAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC2687520.5563618285422569Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TTCAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC2672830.5533207515414214Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGCAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC2631690.5448040798045678Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGTAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC2630750.5446094839992046Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GTCAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC2555380.5290066267117315Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TAGAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC2551810.5282675766849797Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CCGAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC2520780.5218438449398518Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ATGAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC2470990.5115364777600283Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGTAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC2469540.5112363033794148Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCTAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC2443140.5057710594841079Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCGAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC2287390.4735281906699385Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GAGAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC2286550.4733542965459969Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CTTAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC2261330.46813333249146494Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGAAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC2208630.4572235463822725Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGGAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC2191460.45366906768218074Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GCTAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC2170120.4492513288668076Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CTGAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC2133910.44175525002403987Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGTAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC2109390.4366791977394592Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGCAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC2106950.43617407671277175Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GTTAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC2024750.41915729458420203Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ATTAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC1966760.4071523895278061Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GCGAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC1867880.38668256693811054Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GTGAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC1843250.3815837428039661Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TTTAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC1786020.3697361705263744Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TTGAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC1737560.35970413570945853Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGTAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC1727800.35768365160270865Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGGAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC1708900.3537710338140229Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGGAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC1676510.347065759201561Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGGAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC1234910.25564713404369777Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AACAATTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCC727470.15059852183784145Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AAAAATTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCC644850.133494792647301Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ACAAATTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCC606690.12559503101681174Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TACAATTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCC580100.1200904539268036Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CAAAATTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCC564210.11680095674890859Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CACAATTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCC551360.11414079068623072Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TAAAATTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCC527850.10927382538400844Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ACCAATTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCC518320.10730095514452827Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AATAATTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCC508460.10525976935635678Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)

[FAIL]Adapter Content

Adapter graph