FastQCFastQC Report
Sat 30 Sept 2023
SRR15283631_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR15283631_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44069521
Total Bases2.2 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC6593521.496163300708442Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ACACGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC5796601.315330838290709Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TAACGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC5581581.2665397475048572Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AACCGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC5522321.253092812150148Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CAACGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC5390621.2232082123152643Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ATACGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC5120991.1620253371939304Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CCACGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC4828601.095677894933326Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GAACGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC4509291.0232219224710883Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CACCGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC4446821.009046592541816Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TTACGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC4312710.9786151294905158Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ACCCGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC4304830.9768270456127717Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CTACGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC4291900.9738930450367272Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TACCGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC4256540.9658693590066478Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCACGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC4233800.9607093301513306Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AATCGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC4213360.9560712039506851Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AAGCGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC4043890.9176160548693052Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GACCGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC3776890.8570299640878785Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ATCCGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC3659020.830283587606954Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGACGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC3636170.8250985981898918Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ACTCGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC3543950.8041725708795429Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CATCGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC3496400.7933828007797045Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GCACGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC3478690.7893641503387341Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TATCGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC3426030.7774148486887343Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GTACGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC3419490.7759308298358859Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGACGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC3322830.7539973034878232Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CCCCGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC3304110.7497494697071928Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ATGCGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC3298750.7485332096076107Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ATTCGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC3281600.7446416311173429Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CCTCGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC3269970.7420026189982869Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGCCGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC3269680.7419368138809587Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TAGCGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC3267970.7415487906029203Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCCCGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC3099450.7033092100093395Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ACGCGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC3052280.6926056673046208Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGACGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC3051760.6924876719218255Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CAGCGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC2976810.6754804528054661Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TTTCGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC2975770.6752444620398755Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CTTCGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC2969320.6737808654648186Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GCCCGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC2964250.6726304104825647Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CTCCGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC2915280.661518422222016Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GATCGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC2878380.6531452883275042Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TTCCGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC2868470.6508965686284631Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGACGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC2760260.6263421832971591Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GAGCGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC2698060.612228120201261Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGTCGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC2612410.592792919169691Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCTCGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC2605190.5911545986624179Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGCCGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC2605030.5911182923907886Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CTGCGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC2570190.5832126017435043Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGGCGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC2569700.5831014137866395Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TTGCGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC2486250.5641654239899725Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGCCGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC2461250.5584925690478914Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GTTCGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC2451230.5562188887871052Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CCGCGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC2448260.555544953619986Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GTCCGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC2415260.5480567850964388Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GTGCGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC2386550.5415420784809529Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGTCGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC2306330.5233390215428028Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GCTCGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC2235870.5073506471740412Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGCCGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC2234310.5069966610256553Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGTCGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC2184130.49561010658591004Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGGCGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC2150360.4879472141301468Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCGCGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC2093310.47500175915231757Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGTCGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC1884660.42765611180570806Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGGCGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC1845900.41886091750350546Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GCGCGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC1711550.38837499504476125Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGGCGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC1647950.3739432520721067Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AAACGGTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCC696830.15812062037161692Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TAACGGTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCC610000.1384176605867806Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ACACGGTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCC555610.1260757973747888Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CAACGGTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCC541850.12295345801466734Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATACGGTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCC535220.1214490168840274Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AACCGGTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCC516240.11714218541199936Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTACGGTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCC491040.11142394763038156Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CTACGGTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCC483820.1097856271231085Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AAGCGGTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCC463350.10514069349653245Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AATCGGTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCC449990.10210911981548426Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGTCGGTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCC444830.10093824255543872Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)