FastQCFastQC Report
Tue 5 Sept 2023
SRR15324684_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR15324684_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26052634
Total Bases798.8 Mbp
Sequences flagged as poor quality0
Sequence length20-44
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCAC13770735.285734256275201No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCT5156201.9791472908267165No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTT4579831.757914382092805No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCAC4236571.626158030700466No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCAC4123481.5827497519060836No Hit
CGCCGCTGGTGTAGTGGTATCATGCAAGATT2632971.010634855577367No Hit
AGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGC2454050.9419584983230487No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGC1923770.7384166990562259No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAAT1917190.7358910427252768No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAA1870680.7180387211519572No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGC1490880.5722569165175391No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATA1389850.5334777282020697No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAT1359580.5218589414030075No Hit
AGATCCCCGAATCCGGAGTGGCGGAGATGGGCG1326980.5093458112527125No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGG1283630.49270641885960553No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCG1254980.4817094501845764No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATT1243040.47712642030744373No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCA1219160.46796036055317863No Hit
TCGTTTTTTCACTGACCCGGTGAGGCGGGG1190330.4568943009754791No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGA1172970.4502308672512729No Hit
TCGACTGCATAATTTGTGGTAGTGGGGG1115920.42833288948825676No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTT1045840.4014334980486042No Hit
AGATCCCCGAATCCGGAGTGGCGGAGATGGGCGC1039330.3989347104020269No Hit
TACTTGGATAACTGTGGTAATTCTAGAGCT1029880.39530743801183404No Hit
CGACTGCATAATTTGTGGTAGTGGGGGA969080.37197006644318575No Hit
CGACTGCATAATTTGTGGTAGTGGGGG949500.3644545115860454No Hit
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTC933520.35832077478231183No Hit
CTCGGTTGGCCTCGGATAGCCGGTCCCC928160.3562634012361284No Hit
TCTTGCGACCCGGGTTCGTTTCCCGGGCGGCG924710.3549391589349468No Hit
AAATCAGTTATGGTTCCTTTGGTCGCTC886170.3401460289965306No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGA820040.3147627990321439No Hit
AGTCCGCCGAGGGCGCACCACCGGCCCGTCTCG813990.31244057702572414No Hit
TTGCCCTCAGCCGATCGAAAGGGAGTCGGGTTC791970.30398845659905255No Hit
CCTCGGTTGGCCTCGGATAGCCGGTCCCC782280.3002690630052992No Hit
GCCGGCCGAGGTGGGATCCCGAGGCCTCTCC744710.2858482562646065No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCT738340.28340320598677277No Hit
CCGGCCGAGGTGGGATCCCGAGGCCTCTCC736590.28273148887747784No Hit
TTCTTGCGACCCGGGTTCGTTTCCCGGGCGGCG734760.28202906470032935No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCT731270.28068946886522106No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCG727620.27928845889440584No Hit
CGTGATCGTATAGTGGTTAGTACTCTGCGT719740.2762638127108376No Hit
TGCCCTCAGCCGATCGAAAGGGAGTCGGGTTC718230.2756842168051031No Hit
CGGCCGAGGTGGGATCCCGAGGCCTCTCC676830.2597933091909248No Hit
CGACTCTTAGCGGTGGATCACTCGGCTC675830.2594094708427563No Hit
AACGATGCCGACTGGCGATGCGGCGGCGTT670080.257202400340787No Hit
CGTTTTTTCACTGACCCGGTGAGGCGGGG635220.24382179552363115No Hit
GGGAGACCGGGGTTCGATTCCCCGACGGGG633290.2430809875116658No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTT632640.24283149258535627No Hit
ATCGACACTTCGAACGCACTTGCGGCCCCGGGT618670.23746927086144148No Hit
GCCTCGGTTGGCCTCGGATAGCCGGTCCCC603630.23169634210498638No Hit
CCCGGGGCTACGCCTGTCTGAGCGTCGCT589350.2262151304931394No Hit
AACGATGCCGACTGGCGATGCGGCGGCGTTA578910.22220785813825963No Hit
CGCCGGCCGAGGTGGGATCCCGAGGCCTCTCC570440.21895674732927195No Hit
CGCCGGCCGAGGTGGGATCCCGAGGCCT566930.21760947472720032No Hit
CCCTGGTGGTCTAGTGGTTAGGATTCG557700.21406664677360454No Hit
GTACAGTGAAACTGCGAATGGCTCATT554710.21291897011258054No Hit
GGGAGACCGGGGTTCGATTCCCCGACGGG553240.21235472774077277No Hit
CCCTGGTGGTCTAGTGGTTAGGATTCGG544560.2090230108786697No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTC541560.207871495834164No Hit
TCCCACATGGTCTAGCGGTTAGGATTCCTGG540350.20740705143288007No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCG540130.207322606996283No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGG521420.20014099150204925No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGCGT515190.19774967859295914No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTT514540.19750018366664962No Hit
AAATCAGTTATGGTTCCTTTGGTCGCT514260.19739270892916239No Hit
TCTTGCGACCCGGGTTCGTTTCCCGGGCGG513070.1969359412948418No Hit
CTACTTGGATAACTGTGGTAATTCTAGAGCT499340.19166584077448753No Hit
TCCCTGGTGGTCTAGTGGTTAGGATT496130.19043371967686645No Hit
CGTGATCGTATAGTGGTTAGTACTCTGCG489620.18793493203028913No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGG468350.1797706903647439No Hit
TACTTGGATAACTGTGGTAATTCT466570.17908745810500387No Hit
TACCTGGTTGATCCTGCCAGTAGCAT457460.17559069075318834No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTT454910.1746119029653585No Hit
CCCGGGGCTACGCCTGTCTGAGCGTCGCTT447220.1716601860679423No Hit
ACTTCGAACGCACTTGCGGCCCCGGGT446320.1713147315545906No Hit
AACGATGCCGACTGGCGATGCGGCGGCG442990.1700365498551893No Hit
TCCCACATGGTCTAGCGGTTAGGATTCCTGGT440750.16917675195529175No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGGG435960.16733816626756437No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCCG427480.164083217075095No Hit
TCGGTTGGCCTCGGATAGCCGGTCCCC426290.1636264494407744No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGC421420.16175715668519353No Hit
CGGGAGACCGGGGTTCGATTCCCCGACGGG415590.1595193791153708No Hit
CTCGGTTGGCCTCGGATAGCCGGTCCC405660.15570786431805705No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGC405320.15557735927967975No Hit
CGTGATCGTATAGTGGTTAGTACTCTGC397560.15259877369789174No Hit
TCGACTGCATAATTTGTGGTAGTGGGG396860.15233008685417374No Hit
CGGTGGATCACTCGGCTCGTGC392970.15083695567979807No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCA392160.15052604661778154No Hit
CGACTGCATAATTTGTGGTAGTGGGG384520.14759352163777373No Hit
TCCCATATGGTCTAGCGGTTAGGATTCCTGG383060.14703311764944765No Hit
TCTTGCGACCCGGGTTCGTTTCCCGGGCGGC380060.14588160260494198No Hit
CCTCGGTTGGCCTCGGATAGCCGGTCCC374430.14372059270475301No Hit
GCGCCGCTGGTGTAGTGGTATCATGCAAGATT369940.14199715852147618No Hit
GTACAGTGAAACTGCGAATGGCTC369140.14169008784294132No Hit
CTCTAAGTCAGAATCCCGCCCAGGCGGAACGATA360730.13846200733484376No Hit
AGCCCGTGGACGGTGTGAGGCCGGT360470.13836220936431995No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGGG354950.1362434216824295No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCA347690.13345675527472578No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATAG344430.13220544225969627No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGCG338760.13002907882558057No Hit
CGGGAGACCGGGGTTCGATTCCCCGACGGGG335220.12867029107306385No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGC335120.128631907238247No Hit
AGATAACCTCGGGCCGATCGCAC329810.12659372560947196No Hit
CCCTGGTGGTCTAGTGGTTAGGATTCGGCG327830.1258337256800982No Hit
TTCTTGCGACCCGGGTTCGTTTCCCGGGCGG324710.12463615003381233No Hit
CCCTGGTGGTCTAGTGGTTAGGATTC323740.1242638268360888No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGC320630.12307008957328461No Hit
TTCTTGCGACCCGGGTTCGTTTCCCGGGCGGC317430.12184180685914521No Hit
GACCGGGGTCCGGTGCGGAGTGCCCTT316840.12161534223372578No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCG316310.12141190790919644No Hit
CTACTTGGATAACTGTGGTAATTCT315860.1212391806525206No Hit
AAACGATGCCGACTGGCGATGCGGCGGCGTTA312600.11998786763749109No Hit
TTGCGACCCGGGTTCGTTTCCCGGGCGGCG310400.11914342327152026No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGC307790.11814160518280033No Hit
AGTTTTATCCGGTAAAGCGAATGATTAGAGGTC305790.11737392848646322No Hit
CAGAGTTCTACAGTCCGACGATC297100.11403837324087844Illumina DpnII expression Sequencing Primer (100% over 23bp)
CGCCGCTGGTGTAGTGGTATCATGCAAG296500.11380807023197731No Hit
GCCGGCCGAGGTGGGATCCCGAGGCCT295690.11349716116996078No Hit
ACTCCGGATGTGCTGACCCCTGCGA291180.11176605021972058No Hit
TCGCTTCTGGCGCCAAGCGCCCGGCCG290640.11155877751170956No Hit
GCCTCGGTTGGCCTCGGATAGCCGGTCCC284130.10905998986513224No Hit
TCCCATATGGTCTAGCGGTTAGGATTCCTGGT282910.10859170708036661No Hit
TACTTGGATAACTGTGGTAATTCTAGAGCTAATAC280430.1076397879769086No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGC276810.10625029315653842No Hit
TCTAAGTCAGAATCCCGCCCAGGCGGAACGATA269680.1035135257340966No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGG266580.10232362685477407No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAA262580.10078827346209984No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)