FastQCFastQC Report
Tue 5 Sept 2023
SRR15324685_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR15324685_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42945154
Total Bases1.2 Gbp
Sequences flagged as poor quality0
Sequence length20-44
%GC65

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGCCGAGGGCGCACCACCGGCCCGTCTCGC25801966.008119100003693No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCAC10806752.516407322698156No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCG10427242.428036467164607No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCAC4227720.9844463475436599No Hit
AGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGC3619220.8427539926856473No Hit
CGGCCGAGGTGGGATCCCGAGGCCTCTCC3599350.838127160983053No Hit
ACGCGGCGCTCCCCCGGGGAGGGGGGAGGAC3134170.7298076053004724No Hit
CCGGCCGAGGTGGGATCCCGAGGCCTCTCC3049700.7101383313237158No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGC2803070.6527092672668027No Hit
CGACTGCATAATTTGTGGTAGTGGGGG2716270.6324974408055447No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCT2299520.5354550597257144No Hit
AGTCCGCCGAGGGCGCACCACCGGCCCGTCTCG2133090.49670097818254416No Hit
CGCCGGCCGAGGTGGGATCCCGAGGCCT2045180.4762306825119314No Hit
CAGAGTTCTACAGTCCGACGATC1882520.4383544648599933Illumina DpnII expression Sequencing Primer (100% over 23bp)
CGACTGCATAATTTGTGGTAGTGGGG1880060.4377816412068286No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGC1855150.43198121958067726No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCA1664550.3875990292175923No Hit
CGACTGCATAATTTGTGGTAGTGGGGGA1637500.3813002975842164No Hit
ACCTCGGCCGGCGAGCGCGCCGGCCTTC1586380.36939674264528194No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCG1541360.35891360408208106No Hit
ACTGGACGCCTCGCGGCGCCCATCTCCGCC1529710.3562008416595735No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCC1456140.3390696887476524No Hit
ACCTCGGCCGGCGAGCGCGCCGGCCTTCACCTTC1360150.3167179235170515No Hit
TCGCTTCTGGCGCCAAGCGCCCGGCCG1356850.31594950154329404No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTT1354290.31535339237577303No Hit
GAACGGAAGGACGGACGGCGCCGGACGCGC1352840.3150157524176069No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGC1345600.31332988117821164No Hit
ACCGGGGCCCGCGCGGCCAACCCCCGGGACGGGGAC1315760.30638148369429524No Hit
AGAGTTCTACAGTCCGACGATC1237560.288172211467678Illumina DpnII expression Sequencing Primer (100% over 22bp)
GCCGGCCGAGGTGGGATCCCGAGGCCTCTCC1221460.28442324365631566No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCCG1181090.2750228815106822No Hit
TCGACTGCATAATTTGTGGTAGTGGGGG1151400.26810941229830026No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATT1134310.26412991789481066No Hit
TCGCTTCTGGCGCCAAGCGCCCGGCC1112560.259065318522318No Hit
CGCCGCTGGTGTAGTGGTATCATGCAAGATT1062930.24750871774729227No Hit
AGGTTCCCCACGAACGTGCGGTGCGTGACGGGC1034150.2408071467155526No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGC1009740.23512315266118267No Hit
CGCTTCTGGCGCCAAGCGCCCGGCCG993620.23136952774694908No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGG957260.22290291472700274No Hit
GCCGGCCGAGGTGGGATCCCGAGGCCT955780.22255828911452968No Hit
ACCGGACGCCGCCGGAACCGCGACGCTTTCC944780.219996882535338No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCAC943160.2196196572027661No Hit
AACGATGCCGACTGGCGATGCGGCGGCGTT933350.21733534824441425No Hit
ACCGGACGCCGCCGGAACCGCGACGCTTTCCAAGGC930470.21666472543095316No Hit
AACGATGCCGACTGGCGATGCGGCGGCG866990.20188308091758153No Hit
GTGGGGGGCCGGGCCACCCCTCCCACGGCG854800.19904457671755002No Hit
CGCCGGCCGAGGTGGGATCCCGAGGCCTCTCC830390.19336058266318012No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGGG825830.1922987632085334No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCG816610.19015183878488362No Hit
AGTTCTACAGTCCGACGATC774110.1802554951834612No Hit
AAGCTTGGGCTGCAGGTCGACCCGTGC770530.1794218737695061No Hit
TCGACTGCATAATTTGTGGTAGTGGGG759280.17680225340442368No Hit
GAGAGCCCCTCGGGGCTCGCCCCCCCGCCTC739090.17210090805588915No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGA715640.1666404549393396No Hit
TCTTGCGACCCGGGTTCGTTTCCCGGGCGGCG703690.1638578359737632No Hit
GGACGGCGCCGGACGCGCACGCCCCGCCGGGC683830.15923333282260438No Hit
GGAAGGACGGACGGCGCCGGACGCGC669210.15582899062371508No Hit
ACCACCACGCCCGCGGTCGGCGGGAGAGGC666160.15511878243584828No Hit
ACCACGCCCGCGGTCGGCGGGAGAGGC663510.15450171630540666No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCA645550.15031963792701733No Hit
GACTGCATAATTTGTGGTAGTGGGGG620540.14449593078650969No Hit
AACGATGCCGACTGGCGATGCGGCGGCGTTA619580.14427238984868934No Hit
TCTTGCGACCCGGGTTCGTTTCCCGGGCGG552710.12870136639863952No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAAT552690.12869670929576826No Hit
AAGGCACGGGCCCCTCTCTCGGGGCGAACCC548360.12768844652414102No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTATGG547400.12746490558632062No Hit
AACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGC514810.11987615645760637No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAA512320.11929634715013479No Hit
AATCCCCGATCCCCATCACGAATGGGGTTC507250.118115771572271No Hit
GACTGCATAATTTGTGGTAGTGGGG505510.11771060362247157No Hit
AGATCCCCGAATCCGGAGTGGCGGAGATGGGCGC503590.11726352174683086No Hit
CCCGGGGCTACGCCTGTCTGAGCGTCGCT496950.11571736359357333No Hit
GAGTTCTACAGTCCGACGATC489850.11406409207427688Illumina DpnII expression Sequencing Primer (100% over 21bp)
TCTAAGTCAGAATCCCGCCCAGGCGGAACGATA489610.11400820683982178No Hit
ACCCGGGCCCGCGCCCTAGGCTTCAAGGCTCACCGC488140.11366590977878435No Hit
CCCTGGTGGTCTAGTGGTTAGGATTCGGCG484360.11278571733611667No Hit
ACGGCGGCTTTCGTGCGAGCCCCCGACTCGCGC482910.11244807737795048No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTATGGG479250.11159582755251034No Hit
CTCGCTTCTGGCGCCAAGCGCCCGG476070.11085534819598039No Hit
CCCTGGTGGTCTAGTGGTTAGGATTCG475780.11078782020434715No Hit
ACGGGCCCCTCTCTCGGGGCGAACCCATTCC475340.11068536394117949No Hit
GCCGGCCGAGGTGGGATCCCGAGGCCTC474720.1105409937521705No Hit
ACACGCTGAGCCAGTCAGTGTAGCGCGCGTGC448040.10432841852191287No Hit
CCCTGGTGGTCTAGTGGTTAGGATTCGG446230.10390695071206404No Hit
ATCGCCCGTCCCTTCGGAACGGCGCTCGCCC445750.10379518024315386No Hit
ACTCTCGACTGCCGGCGACGGCCGGGT445300.10369039542855057No Hit
GACTGCATAATTTGTGGTAGTGGGGGA433300.10089613370579599No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)