FastQCFastQC Report
Tue 5 Sept 2023
SRR15324686_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR15324686_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences76021896
Total Bases2.2 Gbp
Sequences flagged as poor quality0
Sequence length20-44
%GC67

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGCCGAGGGCGCACCACCGGCCCGTCTCGC55854387.347143775524883No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCG38079825.009059495174917No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCAC8644081.1370513568880207No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGC8579601.1285695900033854No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCG6597070.8677855127422763No Hit
CGGCCGAGGTGGGATCCCGAGGCCTCTCC6217020.8177933368039123No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCT4951970.6513873318813307No Hit
CGACTGCATAATTTGTGGTAGTGGGG4692030.6171945514223955No Hit
ACGCGGCGCTCCCCCGGGGAGGGGGGAGGAC4559300.5997351078957568No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCAC4361860.5737636430430517No Hit
CGACTGCATAATTTGTGGTAGTGGGGG4119950.5419425477102018No Hit
AACGATGCCGACTGGCGATGCGGCGGCG3753870.4937880002361425No Hit
AGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGC3551290.46714041438798104No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTT3225520.4242882866273159No Hit
GCCGGCCGAGGTGGGATCCCGAGGCCT3118920.41026601072932983No Hit
CGCCGGCCGAGGTGGGATCCCGAGGCCT3086110.4059501488886833No Hit
CCGGCCGAGGTGGGATCCCGAGGCCTCTCC3073820.40433350938787427No Hit
TCGCTTCTGGCGCCAAGCGCCCGGCC2913740.3832764181519493No Hit
ACCTCGGCCGGCGAGCGCGCCGGCCTTC2741830.3606631962980771No Hit
AACGATGCCGACTGGCGATGCGGCGGCGTT2654850.3492217557951988No Hit
ACCTCGGCCGGCGAGCGCGCCGGCCTTCACCTTC2535540.3335275931555298No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCC2533870.33330791960253137No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGC2241330.2948269009233866No Hit
AGTCCGCCGAGGGCGCACCACCGGCCCGTCTCG2175610.28618202313712354No Hit
GACTGCATAATTTGTGGTAGTGGGG2157380.28378402980109835No Hit
ACTGGACGCCTCGCGGCGCCCATCTCCGCC2048470.26945789407830606No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGC2042640.2686910097585569No Hit
CGCTTCTGGCGCCAAGCGCCCGGCCG1991710.26199162409735216No Hit
TCGCTTCTGGCGCCAAGCGCCCGGCCG1929970.25387027968889386No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGG1896880.24951758635433138No Hit
CGCCGCTGGTGTAGTGGTATCATGCAAGATT1878570.24710906973433025No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATT1828440.2405149169128852No Hit
CGCTTCTGGCGCCAAGCGCCCGGCC1770900.23294604491316553No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCA1742040.22914977021883273No Hit
GAACGGAAGGACGGACGGCGCCGGACGCGC1715780.2256955022537191No Hit
TCGCTTCTGGCGCCAAGCGCCCGGC1709180.2248273313257012No Hit
GACTGCATAATTTGTGGTAGTGGGGG1659460.21828711033463305No Hit
CGACTGCATAATTTGTGGTAGTGGGGGA1658670.21818319290537033No Hit
CCCGGGGCTACGCCTGTCTGAGCGTCGCT1618240.2128649882660122No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCT1582600.20817686525471554No Hit
AATCCCCGATCCCCATCACGAATGGGGTTC1508040.19836916458910733No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTC1493290.196428934106037No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCG1489660.1959514400956272No Hit
CGAGGGCGCACCACCGGCCCGTCTCGC1487930.19572387407964673No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGGG1487270.19563705698684494No Hit
TCTTGCGACCCGGGTTCGTTTCCCGGGCGGCG1474110.19390597677279714No Hit
CTCGCTTCTGGCGCCAAGCGCCCGG1441780.18965325463600644No Hit
TCGACTGCATAATTTGTGGTAGTGGGG1391900.18309198707698635No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGC1382100.18180288478992948No Hit
CCCTGGTGGTCTAGTGGTTAGGATTCGG1377120.18114781036242505No Hit
CCCGGGGCTACGCCTGTCTGAGCGTCGCTT1351350.17775799751166427No Hit
ACGCACGCGCGCGCGCGCGCGCGGAC1331890.17519820868450847No Hit
AACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGC1314900.1729633262501109No Hit
AAGCTTGGGCTGCAGGTCGACCCGTGC1298990.17087050814938895No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCCG1295610.17042589940140404No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCT1216990.1600841420740151No Hit
ACCGGGGCCCGCGCGGCCAACCCCCGGGACGGGGAC1181680.15543942760911936No Hit
ACCGGACGCCGCCGGAACCGCGACGCTTTCC1162420.1529059469919035No Hit
ACCACGCCCGCGGTCGGCGGGAGAGGC1135930.14942142458535895No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTATGG1133540.1491070414765767No Hit
GAGAGCCCCTCGGGGCTCGCCCCCCCGCCTC1120000.14732597566364303No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAA1110920.1461315829323699No Hit
TCGACTGCATAATTTGTGGTAGTGGGGG1090670.14346787667595134No Hit
CCCTGGTGGTCTAGTGGTTAGGATTCG1090280.14341657566656849No Hit
ACCGGCGGCCCGCAGGGCCGGCGGACCC1079490.14199724774030892No Hit
GAGGGCGCACCACCGGCCCGTCTCGC1067720.14044900958534368No Hit
GGACGGCGCCGGACGCGCACGCCCCGCCGGGC1047570.1377984574338951No Hit
ACGATGCCGACTGGCGATGCGGCGGCG1023230.13459674828420484No Hit
AAACGATGCCGACTGGCGATGCGGCGGCG1004670.13215534640177878No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTT994210.1307794270219201No Hit
GGAAGGACGGACGGCGCCGGACGCGC974140.1281393981544475No Hit
CCGGCCGAGGTGGGATCCCGAGGCCT960640.1263635939835018No Hit
AACGATGCCGACTGGCGATGCGGCGGCGT954200.12551646962343585No Hit
AGGTTCCCCACGAACGTGCGGTGCGTGACGGGC934120.12287512534546625No Hit
AACGATGCCGACTGGCGATGCGGCGGCGTTA924070.12155313779598445No Hit
AGCCCGTGGACGGTGTGAGGCCGGT916560.12056526451273986No Hit
CGATGCCGACTGGCGATGCGGCGGCG906700.119268269762701No Hit
TTGCGACCCGGGTTCGTTTCCCGGGCGGCG898480.11818700233416962No Hit
TCTAAGTCAGAATCCCGCCCAGGCGGAACGATA891980.1173319855111217No Hit
TCTTGCGACCCGGGTTCGTTTCCCGGGCGG888930.11693078530953765No Hit
GCCGGCCGAGGTGGGATCCCGAGGCCTCTCC884720.11637699749030202No Hit
CGCCGCTGGTGTAGTGGTATCATGCAAG881130.11590476512188014No Hit
ACGGGCCCCTCTCTCGGGGCGAACCCATTCC879920.11574560045174354No Hit
ACCACCACGCCCGCGGTCGGCGGGAGAGGC873170.11485769836627069No Hit
CGCCGGCCGAGGTGGGATCCCGAGGCC859250.11302664695445111No Hit
TCGTTTTTTCACTGACCCGGTGAGGCGGGG849990.11180857683423208No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTATGGG827430.10884101075300727No Hit
GTGGGGGGCCGGGCCACCCCTCCCACGGCG827070.10879365597511537No Hit
AAGGCACGGGCCCCTCTCTCGGGGCGAACCC825690.1086121293265298No Hit
AGCGAGCGCACGGGGTCGGCGGCGA816090.10734933524941287No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAT814760.1071743856533123No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCA810010.10654956566723882No Hit
ACGCTGAGCCAGTCAGTGTAGCGCGCGTGC807110.10616809662310975No Hit
TAGTTCAACCTTGAGCTTAC784530.10319789972089094No Hit
ATCGCCCGTCCCTTCGGAACGGCGCTCGCCC783880.10311239803858614No Hit
TTCTTGCGACCCGGGTTCGTTTCCCGGGCGGCG783850.10310845180709516No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATT783310.10303741964025734No Hit
AGGCGTAGCCCCGGGAGGAACCCGGGGCCGC766470.10082226836331469No Hit
ACGCCCGCGGTCGGCGGGAGAGGC765860.10074202832299789No Hit
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGG764370.10054603215894538No Hit
CAGGCGGCCCGGGTTCGACTCCCGGTGTGG760460.10003170665461962No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)