FastQCFastQC Report
Tue 5 Sept 2023
SRR15324687_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR15324687_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences62453066
Total Bases1.8 Gbp
Sequences flagged as poor quality0
Sequence length20-44
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCAC8404041.3456569129848646No Hit
AGTTTTATCCGGTAAAGCGAATGATTAGAGGTC4739710.7589235090555843No Hit
CGCGACCTCAGATCAGACGTGGC4706370.7535851002094917No Hit
ACGCGGCGCTCCCCCGGGGAGGGGGGAGGAC4122440.660086087687032No Hit
AGTAACGGCGAGTGAACAGGGAAGAGCCC4057990.649766338133023No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCG3847070.6159937768307483No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCAC3316510.5310403815883115No Hit
AGGATAGCTGGCGCTCTCGCAAACCC3161620.5062393574080094No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGG3053210.4888807220449353No Hit
TCCCTGGTGGTCTAGTGGTTAGGATT2947350.4719303932972642No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCG2869580.4594778421286794No Hit
AGTTTTATCCGGTAAAGCGAATGATTAGAGGT2787120.4462743270282359No Hit
ATGGAAGTCGGAATCCGCTAAGGAGTGTGTAAC2695880.4316649562088753No Hit
CGACTCTTAGCGGTGGATCACTCGGCTC2679360.4290197698220292No Hit
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTC2584440.41382115651455764No Hit
AGGATTCCCTCAGTAACGGCGAGTGAAC2560500.40998787793700947No Hit
GCCTCGGTTGGCCTCGGATAGCCGGTCCCC2540350.4067614550741192No Hit
GGAAGGACGGACGGCGCCGGACGCGC2498000.3999803628536028No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTC2423810.38810104214899555No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCAC2345350.37553800801389No Hit
GAACGGAAGGACGGACGGCGCCGGACGCGC2321980.3717959979739025No Hit
AAACGATGCCGACTGGCGATGCGGCGGCGTTA2279780.36503892378958624No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGC2265500.3627524067433295No Hit
AGAAAAGGTGTTGGTTGATATAGACAGC2244630.35941069730667824No Hit
ACAGCCAAGGGAACGGGCTTGGCGGAATC2074790.3322158755184253No Hit
TACCTGGTTGATCCTGCCAGTAGC2059220.32972280336084697No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCT2009390.32174401173514844No Hit
AGAAAAGGTGTTGGTTGATATAGAC1960010.3138372742180504No Hit
AGAATCCCGCCCAGGCGGAACGATAC1934210.3097061719916201No Hit
AGAAGGGCAAAAGCTCGCTTGATCTTGATTTTC1854810.29699262482966005No Hit
AGATCTTGGTGGTAGTAGCAAATATTC1810560.28990730415060806No Hit
AGGGGCTCGCACGAAAGCCGCCGTGGCGC1771210.28360657265409517No Hit
CGCCGCTGGTGTAGTGGTATCATGCAAGATT1759510.2817331658304814No Hit
AAACGATGCCGACTGGCGATGCGGCGGCGTT1754450.2809229574093288No Hit
ATCTAGTAGCTGGTTCCCTCCGAAGTTTCCCTC1707140.2733476687917932No Hit
AGTACGAGAGGAACCGCAGGTTCAGAC1683860.2696200695735258No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTT1666570.2668515906008522No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATT1666510.2668419833863721No Hit
GGGAGACCGGGGTTCGATTCCCCGACGGG1619510.2593163320436502No Hit
ACTCTGGTGGAGGTCCGTAGCGGTCCTGACGTGC1603930.2568216586836585No Hit
AGACGTGGCGACCCGCTGAATTTAAGC1574950.25218137408978447No Hit
AGTTATGGTTCCTTTGGTCGCTCG1569100.2512446706779776No Hit
CTCGGTTGGCCTCGGATAGCCGGTCCCC1535880.2459254762608452No Hit
AGAAACCTCCCGTGGAGCAGAAGGGC1519050.24323065259918547No Hit
ATATTAGTCAGCGGAGGAAAAGAAACTAACC1498970.2400154381531885No Hit
CCTCGGTTGGCCTCGGATAGCCGGTCCCC1464480.23449289102956133No Hit
AGCCTCGGTTGGCCTCGGATAGCCGGTCCCC1443180.23108232988913627No Hit
GCCTCGGTTGGCCTCGGATAGCCGGTCCC1417650.22699446012786625No Hit
ACTTCGAACGCACTTGCGGCCCCGGGT1415020.2265733438931565No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTT1402240.22452700720890145No Hit
TCCCACATGGTCTAGCGGTTAGGATTCCTGG1391470.22280251220972883No Hit
ACTCGGCTCGTGCGTCGATGAAGAACGC1369100.21922062241107584No Hit
AAGGAGTCTAACACGTGCGCGAGTC1366380.218785095354646No Hit
AACTTTCGATGGTAGTCGCCGTGCCTACC1347760.21580365646099745No Hit
GTACAGTGAAACTGCGAATGGCTC1331780.213244935004472No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATC1311810.21004733378502186No Hit
AGCGGAGGAAAAGAAACTAACCAGGATTCCCTC1309180.2096262175503121No Hit
AGGATAGCTGGCGCTCTCGCAAACC1305120.208976129370494No Hit
AGGGTCGGGCCTGGTTAGTACTTGGATGGGAGAC1303750.2087567646398657No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGA1296450.20758788687812382No Hit
AGTTAAAAAGCTCGTAGTTGGATCTTGGG1289090.20640940190190185No Hit
AACTTTCGATGGTAGTCGCCGTGCCTACCATGGTGACC1281570.20520529768706633No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGC1279840.20492828966955762No Hit
TACCTGGTTGATCCTGCCAGTAGCAT1261640.20201410127726957No Hit
ATGGTGACCACGGGTGACGGGGAATC1260740.2018699930600685No Hit
CGACTGCATAATTTGTGGTAGTGGGGGA1259180.20162020548358667No Hit
AGACCCCAGAAAAGGTGTTGGTTGATATAGAC1239340.19844341989551No Hit
CGACTGCATAATTTGTGGTAGTGGGGG1234740.19770686678537128No Hit
CTCGCTTCTGGCGCCAAGCGCCCGG1230320.19699913531867277No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAA1223150.1958510731883043No Hit
CGTACGACTCTTAGCGGTGGATC1221080.19551962428874187No Hit
AAAGTAAACGCTTCGGGCCCCGCGG1207410.19333078058969916No Hit
AGGACCCGAAAGATGGTGAACTATGCCTGGGC1203450.1926967044340145No Hit
AGTCAGCGGAGGAAAAGAAACTAACC1202660.19257020944336023No Hit
ATTTCCACCGCGGCGGTGCGCCGCGAC1196460.19157746394708627No Hit
CGGGAGGGAGGAAGACGAACGGAAGGAC1189270.19042619941189118No Hit
CGGGAGACCGGGGTTCGATTCCCCGACGGG1181250.1891420350763884No Hit
AGCTCCAATAGCGTATATTAAAGTTGCTGC1179270.18882499699854607No Hit
AGTTATGGTTCCTTTGGTCGCTC1164630.18648083666540888No Hit
AACGATGCCGACTGGCGATGCGGCGGCGTT1163720.18633512724579449No Hit
ATTGGAGGGCAAGTCTGGTGCCAGC1162330.1861125601103395No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAT1159360.185637002993576No Hit
CGTGATCGTATAGTGGTTAGTACTCTGCG1132550.18134417932339783No Hit
AGGGATAACTGGCTTGTGGCGGCCAAGCGTTC1131350.18115203503379643No Hit
AAGAGGGCGTGAAACCGTTAAGAGGT1131100.1811120049734628No Hit
AAAAGCTCGCTTGATCTTGATTTTC1109120.1775925620689303No Hit
CGATGCCGACTGGCGATGCGGCGGCG1103860.17675032959951076No Hit
GCGCCGCTGGTGTAGTGGTATCATGCAAGATT1101690.1764028686758149No Hit
AGTGAAACTGCGAATGGCTCATTAAATC1092570.17494257207484418No Hit
AACGATGCCGACTGGCGATGCGGCGGCGTTA1070710.17144234359927182No Hit
TCGACTGCATAATTTGTGGTAGTGGGGG1061780.17001246984415463No Hit
GATGGTAGTCGCCGTGCCTACCATGGTGACC1030450.16499590268314449No Hit
AGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGC1015650.16262612311139377No Hit
AGCTAGCTGCGAGAATTAATGTGAATTGCAGGA970580.15540950383444746No Hit
ATTGCACTCCGGATGTGCTGACCCCTGCGA968810.15512609100728536No Hit
CGTTTTTTCACTGACCCGGTGAGGCGGGG940620.1506123014040656No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCCG932510.1493137262468427No Hit
ATGGAAGTCGGAATCCGCTAAGGAGTGTGT930380.1489726701328002No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCC920720.14742590860150886No Hit
GGACGGCGCCGGACGCGCACGCCCCGCCGGGC896890.1436102432505075No Hit
AAATCAGTTATGGTTCCTTTGGTCGCTC896450.14353979034432032No Hit
AGACCCCAGAAAAGGTGTTGGTTGATATAGACAGC894510.14322915707613137No Hit
GGGAGACCGGGGTTCGATTCCCCGACGGGG889680.1424557763104857No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTC875050.14011321717976186No Hit
TTGGATAACTGTGGTAATTCTAGAGCT870160.13933022919963609No Hit
AAACGGTAACGCAGGTGTCCTAAGGCGAGCTC863490.13826222718993492No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTT859690.13765377027286377No Hit
ATGAGAGGTGTAGAATAAGTGGGAGGCCC847360.1356794876972093No Hit
AAAAGCTCGCTTGATCTTGATTTTCAGTACGAATAC841770.1347844155481494No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCT833870.13351946564160677No Hit
AGCCTCGGTTGGCCTCGGATAGCCGGTCCC833120.1333993754606059No Hit
TCCCATATGGTCTAGCGGTTAGGATTCCTGG826440.13232977224849138No Hit
AGGTTAGTTTTACCCTACTGATGATGTGTTGTTGCC815500.13057805680829185No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCA815420.13056524718898507No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAAT812060.13002724317810113No Hit
TGCCCTCAGCCGATCGAAAGGGAGTCGGGTTC808970.12953247163237752No Hit
AGATCAGACGTGGCGACCCGCTGAATTTAAGC808900.12952126321548407No Hit
GACTCTTAGCGGTGGATCACTCGGCTC806190.12908733736146757No Hit
CGGGAGACCGGGGTTCGATTCCCCGACGG802650.1285205117071434No Hit
AGTGCCAGGTGGGGAGTTTGACTGGGGC800960.12824990849928808No Hit
AGCCCGTGGACGGTGTGAGGCCGGT800250.1281362231279406No Hit
CGCGCAGTCCGCCCGGAGGATTC793800.12710344757133302No Hit
AGCTAAGAGCATCGAGGGGGCGCCGAGAGGC786740.12597299866751138No Hit
GGGAGACCGGGGTTCGATTCCCCGACGG783000.12537414896492033No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGC782740.12533251770217335No Hit
ACGGACCAAGGAGTCTAACACGTGCGCGAGTC779810.12486336539506322No Hit
AGGGGAATCCGACTGTTTAATTAAAAC777700.12452551168584741No Hit
CCTCGGTTGGCCTCGGATAGCCGGTCCC773980.12392986438808304No Hit
AGATAACCTCGGGCCGATCGCAC762860.1221493273044433No Hit
AGTTTTATCCGGTAAAGCGAATGATT758070.121382351348451No Hit
ATAAACGATGCCGACTGGCGATGCGGCGGCGTTA756650.121154980605756No Hit
AGCGTTTACTTTGAAAAAATTAGAGTGTTC755590.12098525314994144No Hit
AAATCGGTCGTCCGACCTGGGTAT751840.12038480224493703No Hit
AAAGGGCAGGGACTTAATCAACGC735180.11771719902430411No Hit
AGATCCCCGAATCCGGAGTGGCGGAGATGGGCG730450.11695983028279187No Hit
GGTGTGAGGCCGGTAGCGGCCCCCGGCGC727760.11652910683360206No Hit
CTCGGTTGGCCTCGGATAGCCGGTCCC721060.11545630121666084No Hit
AACGATGCCGACTGGCGATGCGGCGGCG720300.11533460983324663No Hit
GTCTACGGCCATACCACCCTGAAC718850.11510243548331159No Hit
ATCTGTGGGATTATGACTGAACGCCTCTAAGTC708650.11346920902169959No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGC704000.11272464989949413No Hit
ACTCCCGACCCGGGGAGGTAGTGACGAAAAATAAC695450.11135562183608408No Hit
GGTACAGTGAAACTGCGAATGGCTC694390.11118589438026949No Hit
TTGCCCTCAGCCGATCGAAAGGGAGTCGGGTTC692180.11083202864692024No Hit
AGTACGAATACAGACCGTGAAAGCGGGGCCTC685930.10983127713857954No Hit
AGCCAAGGGAACGGGCTTGGCGGAATC684060.10953185228728402No Hit
TCGTTTTTTCACTGACCCGGTGAGGCGGGG671780.10756557572369627No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGC669350.1071764835372534No Hit
ATGTGAACAGCAGTTGAACATGGGTC666120.10665929515774293No Hit
AAGGTGAACAGCCTCTGGCATGTTGGAAC665370.10653920497674205No Hit
CGTGATCGTATAGTGGTTAGTACTCTGC653790.10468501258208845No Hit
GTACAGTGAAACTGCGAATGGCTCATT653180.10458733923487439No Hit
AGGAGGTGTCAGAAAAGTTACCAC650040.10408456167708405No Hit
ACGTGTTAGGACCCGAAAGATGGTGAACT639600.10241290635755176No Hit
GTACGACTCTTAGCGGTGGATC638980.10231363180792437No Hit
TCGCTTCTGGCGCCAAGCGCCCGGCCG637110.10201420695662884No Hit
TCGCTTCTGGCGCCAAGCGCCCGGC634030.10152103661331856No Hit
ACGCGACCTCAGATCAGACGTGGC631460.10110952759308886No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)