FastQCFastQC Report
Tue 5 Sept 2023
SRR15324688_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR15324688_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20759572
Total Bases632.3 Mbp
Sequences flagged as poor quality0
Sequence length20-44
%GC60

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCAC5915412.849485528892407No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCT5848522.8172642480297765No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTT3809251.8349366740316226No Hit
AACGATGCCGACTGGCGATGCGGCGGCGTT2830271.363356624115372No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCT2457001.1835504123110052No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATT2291491.103823335086099No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCT2181711.050941705349224No Hit
AGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGC2173171.0468279403833567No Hit
AACGATGCCGACTGGCGATGCGGCGGCGTTA1951710.9401494404605258No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCAC1944820.9368304895688601No Hit
AAACGATGCCGACTGGCGATGCGGCGGCGTT1925550.9275480245931852No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGC1924860.9272156477985192No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCAC1758720.8471850961089178No Hit
AAACGATGCCGACTGGCGATGCGGCGGCGTTA1718990.828046936613144No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTT1685360.8118471806644182No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTT1654460.7969624807293715No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTT1464960.7056792885710746No Hit
AGATCCCCGAATCCGGAGTGGCGGAGATGGGCG1378520.6640406651929048No Hit
AACGATGCCGACTGGCGATGCGGCGGCG1292140.6224309441447059No Hit
AGATCCCCGAATCCGGAGTGGCGGAGATGGGCGC1280840.6169876720001741No Hit
CGCCGCTGGTGTAGTGGTATCATGCAAGATT1236170.595469887336791No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAT1228840.5919389860253381No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAA1156820.5572465559501901No Hit
GCCTCGGTTGGCCTCGGATAGCCGGTCCCC1066150.5135703183090673No Hit
CCTCGGTTGGCCTCGGATAGCCGGTCCCC1019540.49111802497662277No Hit
TCGTTTTTTCACTGACCCGGTGAGGCGGGG955510.46027442184260836No Hit
CTCGGTTGGCCTCGGATAGCCGGTCCCC921420.4438530813641052No Hit
CGACTGCATAATTTGTGGTAGTGGGGG912830.4397152311232621No Hit
TTGCCCTCAGCCGATCGAAAGGGAGTCGGGTTC876860.4223882843056687No Hit
CCCGGGGCTACGCCTGTCTGAGCGTCGCT875220.42159828728646237No Hit
TAAACGATGCCGACTGGCGATGCGGCGGCGTT808730.3895696886236383No Hit
CGACTCTTAGCGGTGGATCACTCGGCTC804130.3873538433258643No Hit
AACGATGCCGACTGGCGATGCGGCGGCGT777000.3742851731239931No Hit
ATTGATCATCGACACTTCGAACGCA769180.3705182361177774No Hit
TCGACTGCATAATTTGTGGTAGTGGGGG760760.366462275811852No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCA749340.3609611990073784No Hit
CGACTGCATAATTTGTGGTAGTGGGGGA739420.3561826804521789No Hit
AGTCCGCCGAGGGCGCACCACCGGCCCGTCTCG731280.352261597686118No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGA707790.3409463355024853No Hit
CCGGCCGAGGTGGGATCCCGAGGCCTCTCC697250.33586915953758584No Hit
CGCCGGCCGAGGTGGGATCCCGAGGCCT686810.330840154122638No Hit
GCCGGCCGAGGTGGGATCCCGAGGCCTCTCC676470.3258593192576417No Hit
TAAACGATGCCGACTGGCGATGCGGCGGCGTTA672420.32390841198460163No Hit
AAACGATGCCGACTGGCGATGCGGCGGCG652370.31425021671930425No Hit
CGGCCGAGGTGGGATCCCGAGGCCTCTCC640490.3085275553850532No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCG624740.30094069376767496No Hit
CCCGGGGCTACGCCTGTCTGAGCGTCGCTT575120.27703846688168715No Hit
CGTTTTTTCACTGACCCGGTGAGGCGGGG564790.27206244907168603No Hit
ATCGACACTTCGAACGCACTTGCGGCCCCGGGT562760.2710845869076684No Hit
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGG552810.2662916171874834No Hit
CGCCGGCCGAGGTGGGATCCCGAGGCCTCTCC547390.2636807733801063No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGA536340.2583579276104536No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGC530850.2557133644181103No Hit
CTCTAAGTCAGAATCCCGCCCAGGCGGAACGATA524860.25282794847600903No Hit
CGACTGCATAATTTGTGGTAGTGGGG492320.23715325152175581No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTA484820.23354046027538525No Hit
GCCTCGGTTGGCCTCGGATAGCCGGTCCC468210.22553933192842318No Hit
TACTTGGATAACTGTGGTAATTCTAGAGCT452390.2179187509260788No Hit
GCCGGCCGAGGTGGGATCCCGAGGCCT451900.21768271523131597No Hit
TGCCCTCAGCCGATCGAAAGGGAGTCGGGTTC448070.21583778316816934No Hit
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTC443600.2136845595853325No Hit
AGCCCGTGGACGGTGTGAGGCCGGT440020.21196005389706496No Hit
CCTCGGTTGGCCTCGGATAGCCGGTCCC427690.20602062508803168No Hit
GACCGGGGTCCGGTGCGGAGTGCCCTT399710.1925425052115718No Hit
ACTTCGAACGCACTTGCGGCCCCGGGT399410.192397993561717No Hit
AAACGATGCCGACTGGCGATGCGGCGGCGT398980.19219086019692508No Hit
CGATGCCGACTGGCGATGCGGCGGCG395450.1904904397836333No Hit
TCGGTTGGCCTCGGATAGCCGGTCCCC394800.19017733120894784No Hit
AGTTTTATCCGGTAAAGCGAATGATTAGAGGTC385940.1859094204832354No Hit
TTCCCACGCAGTTTTATCCGGTAAAGCG385400.18564929951349673No Hit
TCGACTGCATAATTTGTGGTAGTGGGG383210.1845943644695565No Hit
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGT379380.18274943240640992No Hit
AAATCAGTTATGGTTCCTTTGGTCGCTC371880.17913664116003933No Hit
GACCGGGGTCCGGTGCGGAGTGCCCTTC367160.17686299120232343No Hit
CTCGGTTGGCCTCGGATAGCCGGTCCC365390.17601037246818No Hit
CAGAGTTCTACAGTCCGACGATC348740.1679899759012373Illumina DpnII expression Sequencing Primer (100% over 23bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCG336310.1620023765422524No Hit
GGGAGACCGGGGTTCGATTCCCCGACGGG331220.15955049554971557No Hit
CTCTAAGTCAGAATCCCGCCCAGGCGGAACGATAC320010.15415057690014033No Hit
GGACCGGGGTCCGGTGCGGAGTGCCCTT304980.14691054324241368No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGG304120.14649627651282984No Hit
TACTTGGATAACTGTGGTAATTCT298520.1437987257155398No Hit
GACTGCATAATTTGTGGTAGTGGGGG296310.1427341565616093No Hit
TAAACGATGCCGACTGGCGATGCGGCGGCG293230.14125050362309974No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCCG292460.14087959038847236No Hit
CTTCCCACGCAGTTTTATCCGGTAAAGCG290860.14010886158924662No Hit
CTCTAAGTCAGAATCCCGCCCAGGCGGAACGAT290550.13995953288439666No Hit
TCTAAGTCAGAATCCCGCCCAGGCGGAACGATA289740.13956935142978863No Hit
GCCTCGGTTGGCCTCGGATAGCCGGTC275430.13267614573171355No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCG268940.1295498770398542No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGG268930.12954505998485905No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAAT267590.1288995746155075No Hit
GGGAGACCGGGGTTCGATTCCCCGACGGGG265440.1278639077915479No Hit
AGTTTTATCCGGTAAAGCGAATGATTAGAGGT265120.12770976203170278No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATT259060.12479062670463534No Hit
ACGATGCCGACTGGCGATGCGGCGGCGTT258260.12440526230502247No Hit
TCTTGCGACCCGGGTTCGTTTCCCGGGCGGCG256870.12373569166069512No Hit
CGGGAGACCGGGGTTCGATTCCCCGACGGG248160.11954003675991008No Hit
TTCCCACGCAGTTTTATCCGGTAAAGCGAATGATT247360.11915467236029721No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGC244830.11793595744652155No Hit
CGTGATCGTATAGTGGTTAGTACTCTGCG244310.11768547058677319No Hit
TCGCTTCTGGCGCCAAGCGCCCGGCCG233580.11251677057696566No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCA232920.11219884494728505No Hit
AGCCTCGGTTGGCCTCGGATAGCCGGTCCCC232540.11201579685746893No Hit
ATATGCTTGTCTCAAAGATTAAGCCATG232490.11199171158249313No Hit
TTCTTGCGACCCGGGTTCGTTTCCCGGGCGGCG232440.11196762630751732No Hit
CCCCCCGCGGGGGCGCGCCGGC230200.11088860598860131No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCA229350.11047915631401264No Hit
CCTCGGTTGGCCTCGGATAGCCGGTC226900.10929897784019826No Hit
AAATCAGTTATGGTTCCTTTGGTCGCT226600.10915446619034343No Hit
GTACAGTGAAACTGCGAATGGCTCATT225420.10858605370091445No Hit
GACTCTTAGCGGTGGATCACTCGGCTC220490.10621124558830017No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGC218510.10525746869925834No Hit
TCCCTGGTGGTCTAGTGGTTAGGATT217390.10471795853980034No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATA217280.10466497093485357No Hit
ATTGATCATCGACACTTCGAACGC216990.10452527633999392No Hit
GGACCGGGGTCCGGTGCGGAGTGCCCTTC216870.10446747168005198No Hit
ATTGATCATCGACACTTCGAACGCAC214650.10339808547112628No Hit
ACGATGCCGACTGGCGATGCGGCGGCGTTA214300.10322948854629567No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTC213270.1027333318817941No Hit
ATAAACGATGCCGACTGGCGATGCGGCGGCGTT212760.1024876620770409No Hit
CGGTTGGCCTCGGATAGCCGGTCCCC212460.10234315042718609No Hit
GCGCCGCTGGTGTAGTGGTATCATGCAAGATT209360.10084986337868622No Hit
ACGATGCCGACTGGCGATGCGGCGGCG208390.10038260904415564No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)